A Novel Method for Primary Blood Cell Culturing and Selection in <i>Drosophila melanogaster</i>
The blood cells of the fruit fly <i>Drosophila melanogaster</i> show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study h...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/1/24 |
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| author | Enikő Kúthy-Sutus Bayan Kharrat Erika Gábor Gábor Csordás Rita Sinka Viktor Honti |
| author_facet | Enikő Kúthy-Sutus Bayan Kharrat Erika Gábor Gábor Csordás Rita Sinka Viktor Honti |
| author_sort | Enikő Kúthy-Sutus |
| collection | DOAJ |
| description | The blood cells of the fruit fly <i>Drosophila melanogaster</i> show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the <i>Drosophila</i> larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from <i>Drosophila</i> larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying <i>Drosophila</i> hemocyte differentiation and functions ex vivo. |
| first_indexed | 2024-03-11T10:06:18Z |
| format | Article |
| id | doaj.art-cf96e88ccb74417a976acfc17d47fd3c |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T10:06:18Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-cf96e88ccb74417a976acfc17d47fd3c2023-11-16T15:05:02ZengMDPI AGCells2073-44092022-12-011212410.3390/cells12010024A Novel Method for Primary Blood Cell Culturing and Selection in <i>Drosophila melanogaster</i>Enikő Kúthy-Sutus0Bayan Kharrat1Erika Gábor2Gábor Csordás3Rita Sinka4Viktor Honti5<i>Drosophila</i> Blood Cell Differentiation Group, Institute of Genetics, Biological Research Centre, P.O. Box 521, H-6701 Szeged, Hungary<i>Drosophila</i> Blood Cell Differentiation Group, Institute of Genetics, Biological Research Centre, P.O. Box 521, H-6701 Szeged, Hungary<i>Drosophila</i> Blood Cell Differentiation Group, Institute of Genetics, Biological Research Centre, P.O. Box 521, H-6701 Szeged, HungaryLysosomal Degradation Research Group, Institute of Genetics, Biological Research Centre, P.O. Box 521, H-6701 Szeged, HungaryDepartment of Genetics, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary<i>Drosophila</i> Blood Cell Differentiation Group, Institute of Genetics, Biological Research Centre, P.O. Box 521, H-6701 Szeged, HungaryThe blood cells of the fruit fly <i>Drosophila melanogaster</i> show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the <i>Drosophila</i> larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from <i>Drosophila</i> larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying <i>Drosophila</i> hemocyte differentiation and functions ex vivo.https://www.mdpi.com/2073-4409/12/1/24ex vivo cultureblood cells<i>Drosophila melanogaster</i>lamellocytestransdifferentiation |
| spellingShingle | Enikő Kúthy-Sutus Bayan Kharrat Erika Gábor Gábor Csordás Rita Sinka Viktor Honti A Novel Method for Primary Blood Cell Culturing and Selection in <i>Drosophila melanogaster</i> Cells ex vivo culture blood cells <i>Drosophila melanogaster</i> lamellocytes transdifferentiation |
| title | A Novel Method for Primary Blood Cell Culturing and Selection in <i>Drosophila melanogaster</i> |
| title_full | A Novel Method for Primary Blood Cell Culturing and Selection in <i>Drosophila melanogaster</i> |
| title_fullStr | A Novel Method for Primary Blood Cell Culturing and Selection in <i>Drosophila melanogaster</i> |
| title_full_unstemmed | A Novel Method for Primary Blood Cell Culturing and Selection in <i>Drosophila melanogaster</i> |
| title_short | A Novel Method for Primary Blood Cell Culturing and Selection in <i>Drosophila melanogaster</i> |
| title_sort | novel method for primary blood cell culturing and selection in i drosophila melanogaster i |
| topic | ex vivo culture blood cells <i>Drosophila melanogaster</i> lamellocytes transdifferentiation |
| url | https://www.mdpi.com/2073-4409/12/1/24 |
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