Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)

Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxi...

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Main Authors: Fensia Analda Souhoka, Nikmans Hattu, Marsye Huliselan
Format: Article
Language:English
Published: Department of Chemistry, Pattimura University 2019-07-01
Series:Indonesian Journal of Chemical Research
Subjects:
Online Access:https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/757
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author Fensia Analda Souhoka
Nikmans Hattu
Marsye Huliselan
author_facet Fensia Analda Souhoka
Nikmans Hattu
Marsye Huliselan
author_sort Fensia Analda Souhoka
collection DOAJ
description Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxidant compound and an antioxidant activity of methanol extract of kesumba keling seeds. The moisture content of kesumba keling seeds is 78.74%. The powder of kesumba keling seeds was extracted using maceration method with 80% of methanol. Phytochemical test results of methanol extract positively contained flavonoid compound. An antioxidant activity test of methanol extract of kesumba keling seeds was carried out by determining DPPH free radical deterrent activity. The absorbance measurement were made using a UV-Vis spectrophotometer at a wavelength of 517 nm. Kesumba keling seeds extract has antioxidant activity which is indicated by IC50 value of 69.425 ppm, so it is classified as a strong antioxidant.
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spelling doaj.art-cfa19f5ec51e4e82baa6c5a305244a202022-12-21T20:19:49ZengDepartment of Chemistry, Pattimura UniversityIndonesian Journal of Chemical Research2338-53592614-26272019-07-017110.30598//ijcr.2019.7-fasUji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)Fensia Analda Souhoka0Nikmans Hattu1Marsye Huliselan2Department of Chemistry, Faculty Mathematic and Natural Science, Pattimura University, Jl. Ir. Putuhena No. 1 Poka Ambon 97233-MalukuDepartment of Chemistry, Faculty of Matemathic and Natural Sciences, Pattimura University, Ambon 97233Department of Chemistry, Faculty of Matemathic and Natural Sciences, Pattimura University, Ambon 97233Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxidant compound and an antioxidant activity of methanol extract of kesumba keling seeds. The moisture content of kesumba keling seeds is 78.74%. The powder of kesumba keling seeds was extracted using maceration method with 80% of methanol. Phytochemical test results of methanol extract positively contained flavonoid compound. An antioxidant activity test of methanol extract of kesumba keling seeds was carried out by determining DPPH free radical deterrent activity. The absorbance measurement were made using a UV-Vis spectrophotometer at a wavelength of 517 nm. Kesumba keling seeds extract has antioxidant activity which is indicated by IC50 value of 69.425 ppm, so it is classified as a strong antioxidant.https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/757Antioxidant activity, kesumba keling seeds, methanol extract, phytochemical test.
spellingShingle Fensia Analda Souhoka
Nikmans Hattu
Marsye Huliselan
Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)
Indonesian Journal of Chemical Research
Antioxidant activity, kesumba keling seeds, methanol extract, phytochemical test.
title Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)
title_full Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)
title_fullStr Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)
title_full_unstemmed Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)
title_short Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)
title_sort uji aktivitas antioksidan ekstrak metanol biji kesumba keling bixa orellana l
topic Antioxidant activity, kesumba keling seeds, methanol extract, phytochemical test.
url https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/757
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AT nikmanshattu ujiaktivitasantioksidanekstrakmetanolbijikesumbakelingbixaorellanal
AT marsyehuliselan ujiaktivitasantioksidanekstrakmetanolbijikesumbakelingbixaorellanal