Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin development
Among the different metal oxide nanoparticles, zinc oxide nanoparticles have gained significant importance due to their antibacterial properties against clinically pathogenic bacteria during the organal development. In the present study, biogenic zinc oxide nanoparticles were synthesized using seed...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022016942 |
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| author | Selvakumar Sakthivel Anand Raj Dhanapal Lilly Pushpa Paulraj Annadurai Gurusamy Baskar Venkidasamy Muthu Thiruvengadam Rajakumar Govindasamy Mohammad Ali Shariati Abdelhakim Bouyahya Gokhan Zengin Mohammad Mehedi Hasan Pavel Burkov |
| author_facet | Selvakumar Sakthivel Anand Raj Dhanapal Lilly Pushpa Paulraj Annadurai Gurusamy Baskar Venkidasamy Muthu Thiruvengadam Rajakumar Govindasamy Mohammad Ali Shariati Abdelhakim Bouyahya Gokhan Zengin Mohammad Mehedi Hasan Pavel Burkov |
| author_sort | Selvakumar Sakthivel |
| collection | DOAJ |
| description | Among the different metal oxide nanoparticles, zinc oxide nanoparticles have gained significant importance due to their antibacterial properties against clinically pathogenic bacteria during the organal development. In the present study, biogenic zinc oxide nanoparticles were synthesized using seed extract of Citrus limon by a simple, cost-effective, and green chemistry approach. The synthesized ZnO NPs were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Dynamic Light Scattering, and Scanning Electron Microscopy. Next, the antimicrobial activity of ZnO NPs was tested against clinically pathogenic bacteria, i.e., Pseudomonas fluorescens, Escherichia coli, Enterobacter aerogenes, and Bacillus subtilis. Followed by, ZnO NPs were evaluated for the development of caudal fin in Zebrafish. The UV-Vis spectram result showed a band at 380 nm and FTIR results confirmed the ZnO NPs. The average crystallite size of the ZnO NPs was 52.65 ± 0.5 nm by the Debye Scherrer equation and SEM showed spherical-shaped particles. A zone of inhibition around ZnO NPs applied to P. fluorescens indicates sensitive to ZnO NPs followed by B. subtilis. Among the four different bacterial pathogens, E. aerogenes was the most susceptible compared to the other three pathogens. The calculated sub-lethal concentration of ZnO NPs at 96 h was 153.8 mg/L with a 95% confidence limit ranging from 70.62 to 214.18 mg/L, which was used with partially amputated zebrafish caudal fin growth. A significant (p < 0.5) development (95%) in the amputated caudal fin was detected at 12 days post-amputation. Low concentrated ZnO NPs can reduce developmental malformation. Collectively, suggested results strongly proved that lemon seed-mediated synthesized ZnO NPs had a good pathogenic barrier for bacterial infection during the external organal development for the first time. |
| first_indexed | 2024-04-12T03:45:34Z |
| format | Article |
| id | doaj.art-cfa2bab024dc4f89b17a16a84f27f259 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T03:45:34Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-cfa2bab024dc4f89b17a16a84f27f2592022-12-22T03:49:08ZengElsevierHeliyon2405-84402022-09-0189e10406Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin developmentSelvakumar Sakthivel0Anand Raj Dhanapal1Lilly Pushpa Paulraj2Annadurai Gurusamy3Baskar Venkidasamy4Muthu Thiruvengadam5Rajakumar Govindasamy6Mohammad Ali Shariati7Abdelhakim Bouyahya8Gokhan Zengin9Mohammad Mehedi Hasan10Pavel Burkov11Sri Paramakalyani Centre of Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, 627012, Tamil Nadu, India; Corresponding author.Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, 641021, Tamil Nadu, IndiaSri Paramakalyani Centre of Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, 627012, Tamil Nadu, IndiaSri Paramakalyani Centre of Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, 627012, Tamil Nadu, IndiaDepartment of Oral and Maxillofacial Surgery, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077, Tamil Nadu, IndiaDepartment of Crop Science, College of Sanghuh Life Sciences, Seoul, 05029, South KoreaDepartment of Orthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600 077, Tamil Nadu, IndiaK. G. Razumovsky Moscow State University of Technologies and Management (The First Cossack University), Moscow, 109004, Russian FederationLaboratory of Human Pathologies Biology, Faculty of Sciences, Mohammed V University in Rabat, Rabat, MoroccoDepartment of Biology, Science Faculty, Selcuk University, Campus, Konya, TurkeyDepartment of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh; Corresponding authors.Institute of Veterinary Medicine, South Ural State Agrarian University, 13 Gagarin St., Troitsk, Chelyabinsk Region, 457100, Russian FederationAmong the different metal oxide nanoparticles, zinc oxide nanoparticles have gained significant importance due to their antibacterial properties against clinically pathogenic bacteria during the organal development. In the present study, biogenic zinc oxide nanoparticles were synthesized using seed extract of Citrus limon by a simple, cost-effective, and green chemistry approach. The synthesized ZnO NPs were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Dynamic Light Scattering, and Scanning Electron Microscopy. Next, the antimicrobial activity of ZnO NPs was tested against clinically pathogenic bacteria, i.e., Pseudomonas fluorescens, Escherichia coli, Enterobacter aerogenes, and Bacillus subtilis. Followed by, ZnO NPs were evaluated for the development of caudal fin in Zebrafish. The UV-Vis spectram result showed a band at 380 nm and FTIR results confirmed the ZnO NPs. The average crystallite size of the ZnO NPs was 52.65 ± 0.5 nm by the Debye Scherrer equation and SEM showed spherical-shaped particles. A zone of inhibition around ZnO NPs applied to P. fluorescens indicates sensitive to ZnO NPs followed by B. subtilis. Among the four different bacterial pathogens, E. aerogenes was the most susceptible compared to the other three pathogens. The calculated sub-lethal concentration of ZnO NPs at 96 h was 153.8 mg/L with a 95% confidence limit ranging from 70.62 to 214.18 mg/L, which was used with partially amputated zebrafish caudal fin growth. A significant (p < 0.5) development (95%) in the amputated caudal fin was detected at 12 days post-amputation. Low concentrated ZnO NPs can reduce developmental malformation. Collectively, suggested results strongly proved that lemon seed-mediated synthesized ZnO NPs had a good pathogenic barrier for bacterial infection during the external organal development for the first time.http://www.sciencedirect.com/science/article/pii/S2405844022016942Green synthesisCitrus limon seedZinc oxide nanoparticlesAntibacterial activityZebrafishCaudal fin development |
| spellingShingle | Selvakumar Sakthivel Anand Raj Dhanapal Lilly Pushpa Paulraj Annadurai Gurusamy Baskar Venkidasamy Muthu Thiruvengadam Rajakumar Govindasamy Mohammad Ali Shariati Abdelhakim Bouyahya Gokhan Zengin Mohammad Mehedi Hasan Pavel Burkov Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin development Heliyon Green synthesis Citrus limon seed Zinc oxide nanoparticles Antibacterial activity Zebrafish Caudal fin development |
| title | Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin development |
| title_full | Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin development |
| title_fullStr | Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin development |
| title_full_unstemmed | Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin development |
| title_short | Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin development |
| title_sort | antibacterial activity of seed aqueous extract of citrus limon l mediated synthesis zno nps an impact on zebrafish danio rerio caudal fin development |
| topic | Green synthesis Citrus limon seed Zinc oxide nanoparticles Antibacterial activity Zebrafish Caudal fin development |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022016942 |
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