SmTAL-9, a Member of the Schistosoma mansoni Tegument Allergen-Like Family, Is Important for Parasite Survival and a Putative Target for Drug/Vaccine Development
The tegument of Schistosoma mansoni is involved in essential functions for parasite survival and is known to stimulate immune responses in pre-clinical vaccine trials. Smtal-9, a member of the tegument-allergen-like (TAL) family, is one of the components of the tegument of schistosomula recognized b...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.889645/full |
| _version_ | 1818108410257735680 |
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| author | Wilma Patrícia de Oliveira Santos Bernardes Isabela Thamara Xavier Dutra Rosiane Aparecida da Silva-Pereira Marina Moraes Mourão Cristina Toscano Fonseca |
| author_facet | Wilma Patrícia de Oliveira Santos Bernardes Isabela Thamara Xavier Dutra Rosiane Aparecida da Silva-Pereira Marina Moraes Mourão Cristina Toscano Fonseca |
| author_sort | Wilma Patrícia de Oliveira Santos Bernardes |
| collection | DOAJ |
| description | The tegument of Schistosoma mansoni is involved in essential functions for parasite survival and is known to stimulate immune responses in pre-clinical vaccine trials. Smtal-9, a member of the tegument-allergen-like (TAL) family, is one of the components of the tegument of schistosomula recognized by sera from immunized and protected mice. In this work, we assessed the role of Smtal-9 in parasite survival using the RNAi approach. Also, we cloned and expressed a recombinant form of Smtal-9 and evaluated its ability to induce protection in mice. Smtal-9 knockdown did not impact parasite survival in vitro, but significantly decreased schistosomula size. Additionally, significant reduction in both parasite and egg burdens were observed in mice inoculated with Smtal-9-knockdown schistosomula. Immunization using the Smtal-9 as an antigen conferred partial protection against challenge infection. Overall, our results indicate that Smtal-9 is a candidate target for drug and/or vaccine development due to its important role in parasite biology and survival. |
| first_indexed | 2024-12-11T02:14:55Z |
| format | Article |
| id | doaj.art-cfa3b3170a554f19851fcc61214b6b9e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T02:14:55Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-cfa3b3170a554f19851fcc61214b6b9e2022-12-22T01:24:12ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.889645889645SmTAL-9, a Member of the Schistosoma mansoni Tegument Allergen-Like Family, Is Important for Parasite Survival and a Putative Target for Drug/Vaccine DevelopmentWilma Patrícia de Oliveira Santos Bernardes0Isabela Thamara Xavier Dutra1Rosiane Aparecida da Silva-Pereira2Marina Moraes Mourão3Cristina Toscano Fonseca4Grupo de Pesquisas em Biologia e Imunologia de Doenças Infecciosas e Parasitárias, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, BrazilGrupo de Pesquisas em Helmintologia e Malacologia Médica, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, BrazilGrupo de Pesquisas em Biologia e Imunologia de Doenças Infecciosas e Parasitárias, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, BrazilGrupo de Pesquisas em Helmintologia e Malacologia Médica, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, BrazilGrupo de Pesquisas em Biologia e Imunologia de Doenças Infecciosas e Parasitárias, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, BrazilThe tegument of Schistosoma mansoni is involved in essential functions for parasite survival and is known to stimulate immune responses in pre-clinical vaccine trials. Smtal-9, a member of the tegument-allergen-like (TAL) family, is one of the components of the tegument of schistosomula recognized by sera from immunized and protected mice. In this work, we assessed the role of Smtal-9 in parasite survival using the RNAi approach. Also, we cloned and expressed a recombinant form of Smtal-9 and evaluated its ability to induce protection in mice. Smtal-9 knockdown did not impact parasite survival in vitro, but significantly decreased schistosomula size. Additionally, significant reduction in both parasite and egg burdens were observed in mice inoculated with Smtal-9-knockdown schistosomula. Immunization using the Smtal-9 as an antigen conferred partial protection against challenge infection. Overall, our results indicate that Smtal-9 is a candidate target for drug and/or vaccine development due to its important role in parasite biology and survival.https://www.frontiersin.org/articles/10.3389/fimmu.2022.889645/fullSmtal-9immunizationSchistosoma mansoniRNA interferenceprotection |
| spellingShingle | Wilma Patrícia de Oliveira Santos Bernardes Isabela Thamara Xavier Dutra Rosiane Aparecida da Silva-Pereira Marina Moraes Mourão Cristina Toscano Fonseca SmTAL-9, a Member of the Schistosoma mansoni Tegument Allergen-Like Family, Is Important for Parasite Survival and a Putative Target for Drug/Vaccine Development Frontiers in Immunology Smtal-9 immunization Schistosoma mansoni RNA interference protection |
| title | SmTAL-9, a Member of the Schistosoma mansoni Tegument Allergen-Like Family, Is Important for Parasite Survival and a Putative Target for Drug/Vaccine Development |
| title_full | SmTAL-9, a Member of the Schistosoma mansoni Tegument Allergen-Like Family, Is Important for Parasite Survival and a Putative Target for Drug/Vaccine Development |
| title_fullStr | SmTAL-9, a Member of the Schistosoma mansoni Tegument Allergen-Like Family, Is Important for Parasite Survival and a Putative Target for Drug/Vaccine Development |
| title_full_unstemmed | SmTAL-9, a Member of the Schistosoma mansoni Tegument Allergen-Like Family, Is Important for Parasite Survival and a Putative Target for Drug/Vaccine Development |
| title_short | SmTAL-9, a Member of the Schistosoma mansoni Tegument Allergen-Like Family, Is Important for Parasite Survival and a Putative Target for Drug/Vaccine Development |
| title_sort | smtal 9 a member of the schistosoma mansoni tegument allergen like family is important for parasite survival and a putative target for drug vaccine development |
| topic | Smtal-9 immunization Schistosoma mansoni RNA interference protection |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.889645/full |
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