An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal Sealer
Introduction: Cleaning and shaping of root canal does not guarantee complete disinfection of the canal, regardless of the systems and techniques used for this purpose. Therefore, it becomes mandatory for the endodontic filling materials to have a good antimicrobial potential especially against E...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/8351/20885_CE(Ra1)_F(AK)_PF1(NEAK)_PFA(AK)_PFA2(AK)_PF3(PAG).pdf |
| Summary: | Introduction: Cleaning and shaping of root canal does not
guarantee complete disinfection of the canal, regardless of the
systems and techniques used for this purpose. Therefore, it
becomes mandatory for the endodontic filling materials to have
a good antimicrobial potential especially against E. faecalis.
This research was aimed therefore to evaluate the antibacterial
efficacy and flow properties of different root canal sealers.
Aim: To study invitro antimicrobial activity and flow
characteristics for Resin based (AH Plus), Mineral Trioxide
Aggregate based (MTA Fillapex), Calcium hydroxide based
(CRCS) and Flowable Gutta-Percha (Gutta Flow 2) endodontic
sealers on Enterococcus faecalis.
Materials and Methods: Enterococcus faecalis ATCC 29212 was
used to test antibacterial potential of sealers by Agar diffusion
test (ADT) and Direct Contact Test (DCT). ADT was performed
by punching the sealers on a well of 4×6mm diameter on Muller
Hinton agar plates. These plates were inoculated with standard
suspension of E. faecalis and the zone of inhibition was measured
at 24 hours and after 7 days. All the sealers were prepared in
accordance with the manufacturer’s recommendations. Flow of
sealers was measured according to ADA specification no. 57.
Results: All sealers showed antibacterial activity against E.
faecalis except Gutta Flow 2. At 24 hours, zone of inhibition
was highest in Calcibiotic Root Canal Sealer (CRCS) and lowest
in AH Plus. After 7 days the zone of inhibition decreased in
AH plus, CRCS and MTA Fillapex. DCT showed a significant
lower number of organisms in AH Plus, CRCS and MTA than
controls at both the time intervals. Gutta Flow 2 did not show
any significant antimicrobial action. Maximum and minimum
flow was shown by AH Plus and CRCS respectively.
Conclusion: Highest microbial inhibition was shown by (CRCS),
followed by MTA Fillapex and AH Plus. Gutta Flow 2 did not
show any inhibition of E. faecalis by ADT. Maximum reduction in
antibacterial property with time against E. faecalis was seen with
AH Plus. Maximum flow was shown by AH Plus and minimum
by CRCS. |
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| ISSN: | 2249-782X 0973-709X |