An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal Sealer
Introduction: Cleaning and shaping of root canal does not guarantee complete disinfection of the canal, regardless of the systems and techniques used for this purpose. Therefore, it becomes mandatory for the endodontic filling materials to have a good antimicrobial potential especially against E...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/8351/20885_CE(Ra1)_F(AK)_PF1(NEAK)_PFA(AK)_PFA2(AK)_PF3(PAG).pdf |
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| author | Vijay Kumar Shakya Prashant Gupta Aseem P Tikku Anjani Kumar Pathak Anil Chandra Rakesh Kumar Yadav Ramesh Bharti Rajeev Kumar Singh |
| author_facet | Vijay Kumar Shakya Prashant Gupta Aseem P Tikku Anjani Kumar Pathak Anil Chandra Rakesh Kumar Yadav Ramesh Bharti Rajeev Kumar Singh |
| author_sort | Vijay Kumar Shakya |
| collection | DOAJ |
| description | Introduction: Cleaning and shaping of root canal does not
guarantee complete disinfection of the canal, regardless of the
systems and techniques used for this purpose. Therefore, it
becomes mandatory for the endodontic filling materials to have
a good antimicrobial potential especially against E. faecalis.
This research was aimed therefore to evaluate the antibacterial
efficacy and flow properties of different root canal sealers.
Aim: To study invitro antimicrobial activity and flow
characteristics for Resin based (AH Plus), Mineral Trioxide
Aggregate based (MTA Fillapex), Calcium hydroxide based
(CRCS) and Flowable Gutta-Percha (Gutta Flow 2) endodontic
sealers on Enterococcus faecalis.
Materials and Methods: Enterococcus faecalis ATCC 29212 was
used to test antibacterial potential of sealers by Agar diffusion
test (ADT) and Direct Contact Test (DCT). ADT was performed
by punching the sealers on a well of 4×6mm diameter on Muller
Hinton agar plates. These plates were inoculated with standard
suspension of E. faecalis and the zone of inhibition was measured
at 24 hours and after 7 days. All the sealers were prepared in
accordance with the manufacturer’s recommendations. Flow of
sealers was measured according to ADA specification no. 57.
Results: All sealers showed antibacterial activity against E.
faecalis except Gutta Flow 2. At 24 hours, zone of inhibition
was highest in Calcibiotic Root Canal Sealer (CRCS) and lowest
in AH Plus. After 7 days the zone of inhibition decreased in
AH plus, CRCS and MTA Fillapex. DCT showed a significant
lower number of organisms in AH Plus, CRCS and MTA than
controls at both the time intervals. Gutta Flow 2 did not show
any significant antimicrobial action. Maximum and minimum
flow was shown by AH Plus and CRCS respectively.
Conclusion: Highest microbial inhibition was shown by (CRCS),
followed by MTA Fillapex and AH Plus. Gutta Flow 2 did not
show any inhibition of E. faecalis by ADT. Maximum reduction in
antibacterial property with time against E. faecalis was seen with
AH Plus. Maximum flow was shown by AH Plus and minimum
by CRCS. |
| first_indexed | 2024-12-11T01:01:13Z |
| format | Article |
| id | doaj.art-cfa5fd86f25043c298f86a832dcf8cdd |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-11T01:01:13Z |
| publishDate | 2016-08-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-cfa5fd86f25043c298f86a832dcf8cdd2022-12-22T01:26:20ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-08-01108ZC104ZC10810.7860/JCDR/2016/20885.8351An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal SealerVijay Kumar Shakya0Prashant Gupta1Aseem P Tikku2Anjani Kumar Pathak3Anil Chandra4Rakesh Kumar Yadav5Ramesh Bharti6Rajeev Kumar Singh7Assistant Professor, Department of Conservative Dentistry & Endodontics, Faculty of Dental Sciences, King George’s Medical University, Lucknow, Uttar Pradesh, India.Associate Professor, Department of Microbiology, King George’s Medical University, Lucknow, Uttar Pradesh, India.Professor, Department of Conservative Dentistry & Endodontics, Faculty of Dental Sciences, King George’s Medical University, Lucknow, Uttar Pradesh, India.Assistant Professor, Department of Periodontology, Faculty of Dental Sciences, King George’s Medical University, Lucknow, Uttar Pradesh, India.Professor, Department of Conservative Dentistry & Endodontics, Faculty of Dental Sciences, King George’s Medical University, Lucknow, Uttar Pradesh, India.Associate Professor, Department of Conservative Dentistry & Endodontics, Faculty of Dental Sciences, King George’s Medical University, Lucknow, Uttar Pradesh, India.Assistant Professor, Department of Conservative Dentistry & Endodontics, Faculty of Dental Sciences, King George’s Medical University, Lucknow, Uttar Pradesh, India.Associate Professor, Department of Paediatric & Preventive Dentistry, Faculty of Dental Sciences, King George’s Medical University, Lucknow, Uttar Pradesh, India.Introduction: Cleaning and shaping of root canal does not guarantee complete disinfection of the canal, regardless of the systems and techniques used for this purpose. Therefore, it becomes mandatory for the endodontic filling materials to have a good antimicrobial potential especially against E. faecalis. This research was aimed therefore to evaluate the antibacterial efficacy and flow properties of different root canal sealers. Aim: To study invitro antimicrobial activity and flow characteristics for Resin based (AH Plus), Mineral Trioxide Aggregate based (MTA Fillapex), Calcium hydroxide based (CRCS) and Flowable Gutta-Percha (Gutta Flow 2) endodontic sealers on Enterococcus faecalis. Materials and Methods: Enterococcus faecalis ATCC 29212 was used to test antibacterial potential of sealers by Agar diffusion test (ADT) and Direct Contact Test (DCT). ADT was performed by punching the sealers on a well of 4×6mm diameter on Muller Hinton agar plates. These plates were inoculated with standard suspension of E. faecalis and the zone of inhibition was measured at 24 hours and after 7 days. All the sealers were prepared in accordance with the manufacturer’s recommendations. Flow of sealers was measured according to ADA specification no. 57. Results: All sealers showed antibacterial activity against E. faecalis except Gutta Flow 2. At 24 hours, zone of inhibition was highest in Calcibiotic Root Canal Sealer (CRCS) and lowest in AH Plus. After 7 days the zone of inhibition decreased in AH plus, CRCS and MTA Fillapex. DCT showed a significant lower number of organisms in AH Plus, CRCS and MTA than controls at both the time intervals. Gutta Flow 2 did not show any significant antimicrobial action. Maximum and minimum flow was shown by AH Plus and CRCS respectively. Conclusion: Highest microbial inhibition was shown by (CRCS), followed by MTA Fillapex and AH Plus. Gutta Flow 2 did not show any inhibition of E. faecalis by ADT. Maximum reduction in antibacterial property with time against E. faecalis was seen with AH Plus. Maximum flow was shown by AH Plus and minimum by CRCS.https://jcdr.net/articles/PDF/8351/20885_CE(Ra1)_F(AK)_PF1(NEAK)_PFA(AK)_PFA2(AK)_PF3(PAG).pdfendodontic treatmententerococcus faecalisroot canal obturation |
| spellingShingle | Vijay Kumar Shakya Prashant Gupta Aseem P Tikku Anjani Kumar Pathak Anil Chandra Rakesh Kumar Yadav Ramesh Bharti Rajeev Kumar Singh An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal Sealer Journal of Clinical and Diagnostic Research endodontic treatment enterococcus faecalis root canal obturation |
| title | An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal Sealer |
| title_full | An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal Sealer |
| title_fullStr | An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal Sealer |
| title_full_unstemmed | An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal Sealer |
| title_short | An Invitro Evaluation of Antimicrobial Efficacy and Flow Characteristics for AH Plus, MTA Fillapex, CRCS and Gutta Flow 2 Root Canal Sealer |
| title_sort | invitro evaluation of antimicrobial efficacy and flow characteristics for ah plus mta fillapex crcs and gutta flow 2 root canal sealer |
| topic | endodontic treatment enterococcus faecalis root canal obturation |
| url | https://jcdr.net/articles/PDF/8351/20885_CE(Ra1)_F(AK)_PF1(NEAK)_PFA(AK)_PFA2(AK)_PF3(PAG).pdf |
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