Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities
Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae <i>Pyropia yezoensis</i> and purified by ammoniu...
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/21/6479 |
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| author | Selvakumari Ulagesan Taek-Jeong Nam Youn-Hee Choi |
| author_facet | Selvakumari Ulagesan Taek-Jeong Nam Youn-Hee Choi |
| author_sort | Selvakumari Ulagesan |
| collection | DOAJ |
| description | Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae <i>Pyropia yezoensis</i> and purified by ammonium sulfate precipitation and various chromatography methods. The purified phycoerythrin was analyzed by UV-visible and fluorescence spectroscopy. The isolated pigment had the typical spectrum of R-phycoerythrin, with a trimmer state with absorbance maxima at 497, 536, and 565 nm. It was further purified and identified by LC-MS/MS and Mascot search. It showed a 100% sequence similarity with the R-phycoerythrin alpha subunit of <i>Pyropia yezoensis</i>. The molecular mass was 17.97 kDa. The antioxidant activity of the purified R-phycoerythrin alpha subunit was analyzed. It showed significant antioxidant activity in ABTS and FRAP assays and had significant cytotoxicity against HepG2 cells. |
| first_indexed | 2024-03-10T05:56:12Z |
| format | Article |
| id | doaj.art-cfa64139523249c682ea921ad111ff3c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:56:12Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-cfa64139523249c682ea921ad111ff3c2023-11-22T21:22:02ZengMDPI AGMolecules1420-30492021-10-012621647910.3390/molecules26216479Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological ActivitiesSelvakumari Ulagesan0Taek-Jeong Nam1Youn-Hee Choi2Department of Marine Bio-Materials & Aquaculture, Pukyong National University, Nam-gu, Busan 48513, KoreaInstitute of Fisheries Sciences, Pukyong National University, Gijang-gun, Busan 46041, KoreaDepartment of Marine Bio-Materials & Aquaculture, Pukyong National University, Nam-gu, Busan 48513, KoreaPhycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae <i>Pyropia yezoensis</i> and purified by ammonium sulfate precipitation and various chromatography methods. The purified phycoerythrin was analyzed by UV-visible and fluorescence spectroscopy. The isolated pigment had the typical spectrum of R-phycoerythrin, with a trimmer state with absorbance maxima at 497, 536, and 565 nm. It was further purified and identified by LC-MS/MS and Mascot search. It showed a 100% sequence similarity with the R-phycoerythrin alpha subunit of <i>Pyropia yezoensis</i>. The molecular mass was 17.97 kDa. The antioxidant activity of the purified R-phycoerythrin alpha subunit was analyzed. It showed significant antioxidant activity in ABTS and FRAP assays and had significant cytotoxicity against HepG2 cells.https://www.mdpi.com/1420-3049/26/21/6479<i>Pyropia yezoensis</i>marine algaephycoerythrinantioxidant activitycytotoxicityanticancer activity |
| spellingShingle | Selvakumari Ulagesan Taek-Jeong Nam Youn-Hee Choi Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities Molecules <i>Pyropia yezoensis</i> marine algae phycoerythrin antioxidant activity cytotoxicity anticancer activity |
| title | Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities |
| title_full | Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities |
| title_fullStr | Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities |
| title_full_unstemmed | Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities |
| title_short | Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities |
| title_sort | extraction and purification of r phycoerythrin alpha subunit from the marine red algae i pyropia yezoensis i and its biological activities |
| topic | <i>Pyropia yezoensis</i> marine algae phycoerythrin antioxidant activity cytotoxicity anticancer activity |
| url | https://www.mdpi.com/1420-3049/26/21/6479 |
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