Imidacloprid and thiamethoxam affect synaptic transmission in zebrafish
Imidacloprid (IMI) and thiamethoxam (THM) are two commonly applied neonicotinoid insecticides. IMI and THM could cause negative impacts on non-target organisms like bees. However, the information about neurotoxicity of IMI and THM in fish is still scarce. Here we investigated the effects of IMI and...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321010290 |
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| author | Jin-Ge Zhang Dong-Dong Ma Qian Xiong Shu-Qing Qiu Guo-Yong Huang Wen-Jun Shi Guang-Guo Ying |
| author_facet | Jin-Ge Zhang Dong-Dong Ma Qian Xiong Shu-Qing Qiu Guo-Yong Huang Wen-Jun Shi Guang-Guo Ying |
| author_sort | Jin-Ge Zhang |
| collection | DOAJ |
| description | Imidacloprid (IMI) and thiamethoxam (THM) are two commonly applied neonicotinoid insecticides. IMI and THM could cause negative impacts on non-target organisms like bees. However, the information about neurotoxicity of IMI and THM in fish is still scarce. Here we investigated the effects of IMI and THM on locomotor behavior, AChE activity, and transcription of genes related to synaptic transmission in zebrafish exposed to IMI and THM with concentrations of 50 ng L−1 to 50,000 ng L−1 at 14 day post fertilization (dpf), 21 dpf, 28 dpf and 35 dpf. Our results showed that IMI and THM significantly influenced the locomotor activity in larvae at 28 dpf and 35 dpf. THM elevated AChE activity at 28 dpf. The qPCR data revealed that IMI and THM affected the transcription of marker genes belonging to the synapse from 14 dpf to 35 dpf. Furthermore, IMI and THM mainly affected transcription of key genes in γ-aminobutyric acid, dopamine and serotonin pathways in larvae at 28 dpf and 35 dpf. These results demonstrated the neurotoxicity of IMI and THM in zebrafish. The findings from this study suggested that IMI and THM in the aquatic environment may pose potential risks to fish fitness and survival. |
| first_indexed | 2024-12-14T08:27:18Z |
| format | Article |
| id | doaj.art-cfa92604b1364132b568f9a1bd078665 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-14T08:27:18Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-cfa92604b1364132b568f9a1bd0786652022-12-21T23:09:36ZengElsevierEcotoxicology and Environmental Safety0147-65132021-12-01227112917Imidacloprid and thiamethoxam affect synaptic transmission in zebrafishJin-Ge Zhang0Dong-Dong Ma1Qian Xiong2Shu-Qing Qiu3Guo-Yong Huang4Wen-Jun Shi5Guang-Guo Ying6SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China; Corresponding authors at: SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China; Corresponding authors at: SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, ChinaImidacloprid (IMI) and thiamethoxam (THM) are two commonly applied neonicotinoid insecticides. IMI and THM could cause negative impacts on non-target organisms like bees. However, the information about neurotoxicity of IMI and THM in fish is still scarce. Here we investigated the effects of IMI and THM on locomotor behavior, AChE activity, and transcription of genes related to synaptic transmission in zebrafish exposed to IMI and THM with concentrations of 50 ng L−1 to 50,000 ng L−1 at 14 day post fertilization (dpf), 21 dpf, 28 dpf and 35 dpf. Our results showed that IMI and THM significantly influenced the locomotor activity in larvae at 28 dpf and 35 dpf. THM elevated AChE activity at 28 dpf. The qPCR data revealed that IMI and THM affected the transcription of marker genes belonging to the synapse from 14 dpf to 35 dpf. Furthermore, IMI and THM mainly affected transcription of key genes in γ-aminobutyric acid, dopamine and serotonin pathways in larvae at 28 dpf and 35 dpf. These results demonstrated the neurotoxicity of IMI and THM in zebrafish. The findings from this study suggested that IMI and THM in the aquatic environment may pose potential risks to fish fitness and survival.http://www.sciencedirect.com/science/article/pii/S0147651321010290Neonicotinoid insecticidesNeurotoxicityBehaviorSynaptic transmissionZebrafish |
| spellingShingle | Jin-Ge Zhang Dong-Dong Ma Qian Xiong Shu-Qing Qiu Guo-Yong Huang Wen-Jun Shi Guang-Guo Ying Imidacloprid and thiamethoxam affect synaptic transmission in zebrafish Ecotoxicology and Environmental Safety Neonicotinoid insecticides Neurotoxicity Behavior Synaptic transmission Zebrafish |
| title | Imidacloprid and thiamethoxam affect synaptic transmission in zebrafish |
| title_full | Imidacloprid and thiamethoxam affect synaptic transmission in zebrafish |
| title_fullStr | Imidacloprid and thiamethoxam affect synaptic transmission in zebrafish |
| title_full_unstemmed | Imidacloprid and thiamethoxam affect synaptic transmission in zebrafish |
| title_short | Imidacloprid and thiamethoxam affect synaptic transmission in zebrafish |
| title_sort | imidacloprid and thiamethoxam affect synaptic transmission in zebrafish |
| topic | Neonicotinoid insecticides Neurotoxicity Behavior Synaptic transmission Zebrafish |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321010290 |
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