Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids
Near-infrared photoacoustics receives increasing interest as an intravital modality to sense key biomolecules. One of the most central types of biomolecules of interest are lipids as they constitute essential bio-hallmarks of cardiovascular and metabolic diseases and their in-vivo detection holds in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597922000039 |
| _version_ | 1818986621164322816 |
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| author | Hwidon Lee Markus R. Seeger Norman Lippok Seemantini K. Nadkarni Gijs van Soest Brett E. Bouma |
| author_facet | Hwidon Lee Markus R. Seeger Norman Lippok Seemantini K. Nadkarni Gijs van Soest Brett E. Bouma |
| author_sort | Hwidon Lee |
| collection | DOAJ |
| description | Near-infrared photoacoustics receives increasing interest as an intravital modality to sense key biomolecules. One of the most central types of biomolecules of interest are lipids as they constitute essential bio-hallmarks of cardiovascular and metabolic diseases and their in-vivo detection holds insightful information about disease progression and treatment monitoring. However, the full potential of near-infrared photoacoustic for high-resolution and high-sensitivity biomedical studies of lipids has so far not been exploited due a lack of appropriate excitation sources delivering short-pulses at high-repetition-rate, high-pulse-energy, and wavelength around 1200 nm. Here, we demonstrate a custom-built SRS fiber amplifier that provides optical excitations at 1192.8 nm, repetition rates of 200 kHz, pulse durations below 2 ns, and pulse energies beyond 5 μJ. We capitalize on the performance of our excitation source and show near-infrared photoacoustics resolving intrinsic lipid contrast in biomedically relevant specimens ranging from single cells to lipid-rich tissue with subcellular resolution. |
| first_indexed | 2024-12-20T18:53:42Z |
| format | Article |
| id | doaj.art-cfacbb0d86674445b29418b4f65ec901 |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-12-20T18:53:42Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-cfacbb0d86674445b29418b4f65ec9012022-12-21T19:29:34ZengElsevierPhotoacoustics2213-59792022-03-0125100331Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipidsHwidon Lee0Markus R. Seeger1Norman Lippok2Seemantini K. Nadkarni3Gijs van Soest4Brett E. Bouma5Harvard Medical School, Boston, Massachusetts, MA 02115, USA; Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USAHarvard Medical School, Boston, Massachusetts, MA 02115, USA; Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USAHarvard Medical School, Boston, Massachusetts, MA 02115, USA; Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USAHarvard Medical School, Boston, Massachusetts, MA 02115, USA; Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USADepartment of Biomedical Engineering, Erasmus Medical Center, PO Box 2040, 3000 Rotterdam, CA The NetherlandsHarvard Medical School, Boston, Massachusetts, MA 02115, USA; Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA; Department of Biomedical Engineering, Erasmus Medical Center, PO Box 2040, 3000 Rotterdam, CA The Netherlands; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Corresponding author at: Harvard Medical School, Boston, Massachusetts, MA 02115, USA.Near-infrared photoacoustics receives increasing interest as an intravital modality to sense key biomolecules. One of the most central types of biomolecules of interest are lipids as they constitute essential bio-hallmarks of cardiovascular and metabolic diseases and their in-vivo detection holds insightful information about disease progression and treatment monitoring. However, the full potential of near-infrared photoacoustic for high-resolution and high-sensitivity biomedical studies of lipids has so far not been exploited due a lack of appropriate excitation sources delivering short-pulses at high-repetition-rate, high-pulse-energy, and wavelength around 1200 nm. Here, we demonstrate a custom-built SRS fiber amplifier that provides optical excitations at 1192.8 nm, repetition rates of 200 kHz, pulse durations below 2 ns, and pulse energies beyond 5 μJ. We capitalize on the performance of our excitation source and show near-infrared photoacoustics resolving intrinsic lipid contrast in biomedically relevant specimens ranging from single cells to lipid-rich tissue with subcellular resolution.http://www.sciencedirect.com/science/article/pii/S2213597922000039Fiber amplifierPhotoacoustic microscopyStimulated Raman scatteringLipidsLabel-free imagingNear-Infrared |
| spellingShingle | Hwidon Lee Markus R. Seeger Norman Lippok Seemantini K. Nadkarni Gijs van Soest Brett E. Bouma Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids Photoacoustics Fiber amplifier Photoacoustic microscopy Stimulated Raman scattering Lipids Label-free imaging Near-Infrared |
| title | Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids |
| title_full | Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids |
| title_fullStr | Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids |
| title_full_unstemmed | Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids |
| title_short | Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids |
| title_sort | nanosecond srs fiber amplifier for label free near infrared photoacoustic microscopy of lipids |
| topic | Fiber amplifier Photoacoustic microscopy Stimulated Raman scattering Lipids Label-free imaging Near-Infrared |
| url | http://www.sciencedirect.com/science/article/pii/S2213597922000039 |
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