First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS).
Dippity Pig Syndrome (DPS) is a well-known but rare complex of clinical signs affecting minipigs, which has not been thoroughly investigated yet. Clinically affected animals show acute appearance of red, exudating lesions across the spine. The lesions are painful, evidenced by arching of the back (d...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0281521 |
| _version_ | 1797394435780116480 |
|---|---|
| author | Hina Jhelum Nanna Grand Kirsten Rosenmay Jacobsen Sabrina Halecker Michelle Salerno Robert Prate Luise Krüger Yannick Kristiansen Ludwig Krabben Lars Möller Michael Laue Benedikt Kaufer Kari Kaaber Joachim Denner |
| author_facet | Hina Jhelum Nanna Grand Kirsten Rosenmay Jacobsen Sabrina Halecker Michelle Salerno Robert Prate Luise Krüger Yannick Kristiansen Ludwig Krabben Lars Möller Michael Laue Benedikt Kaufer Kari Kaaber Joachim Denner |
| author_sort | Hina Jhelum |
| collection | DOAJ |
| description | Dippity Pig Syndrome (DPS) is a well-known but rare complex of clinical signs affecting minipigs, which has not been thoroughly investigated yet. Clinically affected animals show acute appearance of red, exudating lesions across the spine. The lesions are painful, evidenced by arching of the back (dipping), and the onset of clinical signs is generally sudden. In order to understand the pathogenesis, histological and virological investigations were performed in affected and unaffected Göttingen Minipigs (GöMPs). The following DNA viruses were screened for using PCR-based methods: Porcine cytomegalovirus (PCMV), which is a porcine roseolovirus (PCMV/PRV), porcine lymphotropic herpesviruses (PLHV-1, PLHV-2, PLHV-3), porcine circoviruses (PCV1, PCV2, PCV3, PCV4), porcine parvovirus 1 (PPV1), and Torque Teno sus viruses (TTSuV1, TTSuV2). Screening was also performed for integrated porcine endogenous retroviruses (PERV-A, PERV-B, PERV-C) and recombinant PERV-A/C and their expression as well as for the RNA viruses hepatitis E virus (HEV) and SARS-CoV-2. Eight clinically affected and one unaffected GöMPs were analyzed. Additional unaffected minipigs had been analyzed in the past. The analyzed GöMPs contained PERV-A and PERV-B integrated in the genome, which are present in all pigs and PERV-C, which is present in most, but not all pigs. In one affected GöMPs recombinant PERV-A/C was detected in blood. In this animal a very high expression of PERV mRNA was observed. PCMV/PRV was found in three affected animals, PCV1 was found in three animals with DPS and in the unaffected minipig, and PCV3 was detected in two animals with DPS and in the unaffected minipig. Most importantly, in one animal only PLHV-3 was detected. It was found in the affected and unaffected skin, and in other organs. Unfortunately, PLHV-3 could not be studied in all other affected minipigs. None of the other viruses were detected and using electron microscopy, no virus particles were found in the affected skin. No porcine virus RNA with exception of PERV and astrovirus RNA were detected in the affected skin by next generation sequencing. This data identified some virus infections in GöMPs with DPS and assign a special role to PLHV-3. Since PCMV/PRV, PCV1, PCV3 and PLHV-3 were also found in unaffected animals, a multifactorial cause of DPS is suggested. However, elimination of the viruses from GöMPs may prevent DPS. |
| first_indexed | 2024-03-09T00:19:41Z |
| format | Article |
| id | doaj.art-cfae4b08fadb4b5882f74d4bc8b7f324 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-09T00:19:41Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cfae4b08fadb4b5882f74d4bc8b7f3242023-12-12T05:36:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01186e028152110.1371/journal.pone.0281521First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS).Hina JhelumNanna GrandKirsten Rosenmay JacobsenSabrina HaleckerMichelle SalernoRobert PrateLuise KrügerYannick KristiansenLudwig KrabbenLars MöllerMichael LaueBenedikt KauferKari KaaberJoachim DennerDippity Pig Syndrome (DPS) is a well-known but rare complex of clinical signs affecting minipigs, which has not been thoroughly investigated yet. Clinically affected animals show acute appearance of red, exudating lesions across the spine. The lesions are painful, evidenced by arching of the back (dipping), and the onset of clinical signs is generally sudden. In order to understand the pathogenesis, histological and virological investigations were performed in affected and unaffected Göttingen Minipigs (GöMPs). The following DNA viruses were screened for using PCR-based methods: Porcine cytomegalovirus (PCMV), which is a porcine roseolovirus (PCMV/PRV), porcine lymphotropic herpesviruses (PLHV-1, PLHV-2, PLHV-3), porcine circoviruses (PCV1, PCV2, PCV3, PCV4), porcine parvovirus 1 (PPV1), and Torque Teno sus viruses (TTSuV1, TTSuV2). Screening was also performed for integrated porcine endogenous retroviruses (PERV-A, PERV-B, PERV-C) and recombinant PERV-A/C and their expression as well as for the RNA viruses hepatitis E virus (HEV) and SARS-CoV-2. Eight clinically affected and one unaffected GöMPs were analyzed. Additional unaffected minipigs had been analyzed in the past. The analyzed GöMPs contained PERV-A and PERV-B integrated in the genome, which are present in all pigs and PERV-C, which is present in most, but not all pigs. In one affected GöMPs recombinant PERV-A/C was detected in blood. In this animal a very high expression of PERV mRNA was observed. PCMV/PRV was found in three affected animals, PCV1 was found in three animals with DPS and in the unaffected minipig, and PCV3 was detected in two animals with DPS and in the unaffected minipig. Most importantly, in one animal only PLHV-3 was detected. It was found in the affected and unaffected skin, and in other organs. Unfortunately, PLHV-3 could not be studied in all other affected minipigs. None of the other viruses were detected and using electron microscopy, no virus particles were found in the affected skin. No porcine virus RNA with exception of PERV and astrovirus RNA were detected in the affected skin by next generation sequencing. This data identified some virus infections in GöMPs with DPS and assign a special role to PLHV-3. Since PCMV/PRV, PCV1, PCV3 and PLHV-3 were also found in unaffected animals, a multifactorial cause of DPS is suggested. However, elimination of the viruses from GöMPs may prevent DPS.https://doi.org/10.1371/journal.pone.0281521 |
| spellingShingle | Hina Jhelum Nanna Grand Kirsten Rosenmay Jacobsen Sabrina Halecker Michelle Salerno Robert Prate Luise Krüger Yannick Kristiansen Ludwig Krabben Lars Möller Michael Laue Benedikt Kaufer Kari Kaaber Joachim Denner First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS). PLoS ONE |
| title | First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS). |
| title_full | First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS). |
| title_fullStr | First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS). |
| title_full_unstemmed | First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS). |
| title_short | First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS). |
| title_sort | first virological and pathological study of gottingen minipigs with dippity pig syndrome dps |
| url | https://doi.org/10.1371/journal.pone.0281521 |
| work_keys_str_mv | AT hinajhelum firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT nannagrand firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT kirstenrosenmayjacobsen firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT sabrinahalecker firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT michellesalerno firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT robertprate firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT luisekruger firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT yannickkristiansen firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT ludwigkrabben firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT larsmoller firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT michaellaue firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT benediktkaufer firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT karikaaber firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps AT joachimdenner firstvirologicalandpathologicalstudyofgottingenminipigswithdippitypigsyndromedps |