Priming European Sea Bass Female Broodstock Improves the Antimicrobial Immunity of Their Offspring
Acquiring immunocompetence is essential in the development of fish embryos, as they are exposed to environmental pathogens even before they are fertilized. Despite the importance of the antimicrobial function as the first line of defense against foreign microorganisms, little knowledge is available...
| Κύριοι συγγραφείς: | , , , , |
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| Μορφή: | Άρθρο |
| Γλώσσα: | English |
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MDPI AG
2023-01-01
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| Σειρά: | Animals |
| Θέματα: | |
| Διαθέσιμο Online: | https://www.mdpi.com/2076-2615/13/3/415 |
| _version_ | 1827760642136735744 |
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| author | Yulema Valero Luis Mercado Marta Arizcun Alberto Cuesta Elena Chaves-Pozo |
| author_facet | Yulema Valero Luis Mercado Marta Arizcun Alberto Cuesta Elena Chaves-Pozo |
| author_sort | Yulema Valero |
| collection | DOAJ |
| description | Acquiring immunocompetence is essential in the development of fish embryos, as they are exposed to environmental pathogens even before they are fertilized. Despite the importance of the antimicrobial function as the first line of defense against foreign microorganisms, little knowledge is available about its role in larval development. In vertebrates, transgenerational immune priming influences the acquisition of immunocompetence of specimens, regulating the selective allocation of nongenetic resources to their progeny and modulating their development. In this work, we primed teleost European sea bass broodstock females with a viral protein expression vector in order to evaluate the innate immunity development of their offspring. Several antimicrobial functions, the pattern of expression of gene coding for different antimicrobial peptides (AMPs), and their protein levels, were evaluated in eggs and larvae during development. Our data determined the presence of antimicrobial proteins of maternal origin in eggs, and that female vaccination increases antimicrobial activities and the transcription and synthesis of AMPs during larval development. |
| first_indexed | 2024-03-11T09:54:25Z |
| format | Article |
| id | doaj.art-cfaef8535652490f8d3156b966b2d63e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:54:25Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-cfaef8535652490f8d3156b966b2d63e2023-11-16T16:00:32ZengMDPI AGAnimals2076-26152023-01-0113341510.3390/ani13030415Priming European Sea Bass Female Broodstock Improves the Antimicrobial Immunity of Their OffspringYulema Valero0Luis Mercado1Marta Arizcun2Alberto Cuesta3Elena Chaves-Pozo4Oceanographic Centre of Murcia, Spanish Institute of Oceanography, Spanish National Research Council, Carretera de la Azohía s/n, Puerto de Mazarrón, 30860 Murcia, SpainGrupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2362807, ChileOceanographic Centre of Murcia, Spanish Institute of Oceanography, Spanish National Research Council, Carretera de la Azohía s/n, Puerto de Mazarrón, 30860 Murcia, SpainImmunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, SpainOceanographic Centre of Murcia, Spanish Institute of Oceanography, Spanish National Research Council, Carretera de la Azohía s/n, Puerto de Mazarrón, 30860 Murcia, SpainAcquiring immunocompetence is essential in the development of fish embryos, as they are exposed to environmental pathogens even before they are fertilized. Despite the importance of the antimicrobial function as the first line of defense against foreign microorganisms, little knowledge is available about its role in larval development. In vertebrates, transgenerational immune priming influences the acquisition of immunocompetence of specimens, regulating the selective allocation of nongenetic resources to their progeny and modulating their development. In this work, we primed teleost European sea bass broodstock females with a viral protein expression vector in order to evaluate the innate immunity development of their offspring. Several antimicrobial functions, the pattern of expression of gene coding for different antimicrobial peptides (AMPs), and their protein levels, were evaluated in eggs and larvae during development. Our data determined the presence of antimicrobial proteins of maternal origin in eggs, and that female vaccination increases antimicrobial activities and the transcription and synthesis of AMPs during larval development.https://www.mdpi.com/2076-2615/13/3/415broodstockprotein-expressing vectorantimicrobial peptidesantimicrobial functioninnate immune response<i>Dicentrarchus labrax</i> |
| spellingShingle | Yulema Valero Luis Mercado Marta Arizcun Alberto Cuesta Elena Chaves-Pozo Priming European Sea Bass Female Broodstock Improves the Antimicrobial Immunity of Their Offspring Animals broodstock protein-expressing vector antimicrobial peptides antimicrobial function innate immune response <i>Dicentrarchus labrax</i> |
| title | Priming European Sea Bass Female Broodstock Improves the Antimicrobial Immunity of Their Offspring |
| title_full | Priming European Sea Bass Female Broodstock Improves the Antimicrobial Immunity of Their Offspring |
| title_fullStr | Priming European Sea Bass Female Broodstock Improves the Antimicrobial Immunity of Their Offspring |
| title_full_unstemmed | Priming European Sea Bass Female Broodstock Improves the Antimicrobial Immunity of Their Offspring |
| title_short | Priming European Sea Bass Female Broodstock Improves the Antimicrobial Immunity of Their Offspring |
| title_sort | priming european sea bass female broodstock improves the antimicrobial immunity of their offspring |
| topic | broodstock protein-expressing vector antimicrobial peptides antimicrobial function innate immune response <i>Dicentrarchus labrax</i> |
| url | https://www.mdpi.com/2076-2615/13/3/415 |
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