Genetic Diversity of Populations in Serbia
In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2021-12-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-04-2021-0066.pdf |
| _version_ | 1811308526680670208 |
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| author | Žarko Ivanović Dragana Marisavljević Radovan Marinković Petar Mitrović Jovana Blagojević Ivan Nikolić Danijela Pavlović |
| author_facet | Žarko Ivanović Dragana Marisavljević Radovan Marinković Petar Mitrović Jovana Blagojević Ivan Nikolić Danijela Pavlović |
| author_sort | Žarko Ivanović |
| collection | DOAJ |
| description | In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control. |
| first_indexed | 2024-04-13T09:24:52Z |
| format | Article |
| id | doaj.art-cfb05f25c20f43649fa987246117f4eb |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-13T09:24:52Z |
| publishDate | 2021-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-cfb05f25c20f43649fa987246117f4eb2022-12-22T02:52:29ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802021-12-0137651252010.5423/PPJ.OA.04.2021.00662280Genetic Diversity of Populations in SerbiaŽarko Ivanović0Dragana Marisavljević1Radovan Marinković2Petar Mitrović3Jovana Blagojević4Ivan Nikolić5Danijela Pavlović6 Institute for Plant Protection and Environment, 11000 Belgrade, Serbia Institute for Plant Protection and Environment, 11000 Belgrade, Serbia Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia Institute for Plant Protection and Environment, 11000 Belgrade, Serbia Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia Institute for Plant Protection and Environment, 11000 Belgrade, SerbiaIn this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.http://www.ppjonline.org/upload/pdf/PPJ-OA-04-2021-0066.pdfintrapopulation variationphylogenyrapdsunflower broomrape |
| spellingShingle | Žarko Ivanović Dragana Marisavljević Radovan Marinković Petar Mitrović Jovana Blagojević Ivan Nikolić Danijela Pavlović Genetic Diversity of Populations in Serbia The Plant Pathology Journal intrapopulation variation phylogeny rapd sunflower broomrape |
| title | Genetic Diversity of Populations in Serbia |
| title_full | Genetic Diversity of Populations in Serbia |
| title_fullStr | Genetic Diversity of Populations in Serbia |
| title_full_unstemmed | Genetic Diversity of Populations in Serbia |
| title_short | Genetic Diversity of Populations in Serbia |
| title_sort | genetic diversity of populations in serbia |
| topic | intrapopulation variation phylogeny rapd sunflower broomrape |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-04-2021-0066.pdf |
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