Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes
Abstract Metalloproteinases (MMPs) are remarkable zinc-dependent endopeptidases, critical for degrading components of the extracellular matrix, thus actively influencing cell migration. Their impact on intracellular parasites, such as the enigmatic protozoan Leishmania, elicits intriguing queries. T...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02973-z |
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| author | Brenda Furtado Costa Tarcísio Navegante de Queiroz Filho Adeniele Lopes da Cruz Carneiro Aline Semblano Carreira Falcão Maria Sueli da Silva Kataoka João de Jesus Viana Pinheiro Ana Paula Drummond Rodrigues |
| author_facet | Brenda Furtado Costa Tarcísio Navegante de Queiroz Filho Adeniele Lopes da Cruz Carneiro Aline Semblano Carreira Falcão Maria Sueli da Silva Kataoka João de Jesus Viana Pinheiro Ana Paula Drummond Rodrigues |
| author_sort | Brenda Furtado Costa |
| collection | DOAJ |
| description | Abstract Metalloproteinases (MMPs) are remarkable zinc-dependent endopeptidases, critical for degrading components of the extracellular matrix, thus actively influencing cell migration. Their impact on intracellular parasites, such as the enigmatic protozoan Leishmania, elicits intriguing queries. This study explores into the untapped territory of MMP-2 and MMP-9 within Leishmania spp. promastigotes. Notably, we successfully detected and quantified these MMPs, while also evaluating their activity in two distinct Leishmania species—L. amazonensis (La) and L. braziliensis (Lb)—at various growth stages and isolated from distinct clinical tegumentar disease forms. The results unveiled the presence of MMP-2 and MMP-9 in both species, albeit with distinct localization patterns. Specifically, MMP-9 exhibited significantly higher gelatinolytic activity in La when compared to Lb. Moreover, our data cleverly illustrated the presence and release of MMP-2 and MMP-9 by La and Lb promastigotes, exposing their ability to invade and migrate within a collagen matrix. This pioneering study establishes a compelling correlation between MMP-2 and MMP-9 and their potential role in the dynamics of La and Lb infection. Suggesting their potential as prognostic markers for severe leishmaniasis and promising target molecules for therapeutic interventions, this research opens new avenues for combatting this debilitating parasitic disease. |
| first_indexed | 2024-03-09T15:26:42Z |
| format | Article |
| id | doaj.art-cfb194d53e6d4310b2d1143b8a7a1eac |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-09T15:26:42Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-cfb194d53e6d4310b2d1143b8a7a1eac2023-11-26T12:30:15ZengBMCBMC Microbiology1471-21802023-08-0123111210.1186/s12866-023-02973-zDetection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotesBrenda Furtado Costa0Tarcísio Navegante de Queiroz Filho1Adeniele Lopes da Cruz Carneiro2Aline Semblano Carreira Falcão3Maria Sueli da Silva Kataoka4João de Jesus Viana Pinheiro5Ana Paula Drummond Rodrigues6Laboratory of Electron Microscopy, Section of Hepatology, Evandro Chagas InstituteLaboratory of Electron Microscopy, Section of Hepatology, Evandro Chagas InstituteLaboratory of Electron Microscopy, Section of Hepatology, Evandro Chagas InstituteDepartment of Oral Pathology, School of Dentistry, Federal University of ParaDepartment of Oral Pathology, School of Dentistry, Federal University of ParaDepartment of Oral Pathology, School of Dentistry, Federal University of ParaLaboratory of Electron Microscopy, Section of Hepatology, Evandro Chagas InstituteAbstract Metalloproteinases (MMPs) are remarkable zinc-dependent endopeptidases, critical for degrading components of the extracellular matrix, thus actively influencing cell migration. Their impact on intracellular parasites, such as the enigmatic protozoan Leishmania, elicits intriguing queries. This study explores into the untapped territory of MMP-2 and MMP-9 within Leishmania spp. promastigotes. Notably, we successfully detected and quantified these MMPs, while also evaluating their activity in two distinct Leishmania species—L. amazonensis (La) and L. braziliensis (Lb)—at various growth stages and isolated from distinct clinical tegumentar disease forms. The results unveiled the presence of MMP-2 and MMP-9 in both species, albeit with distinct localization patterns. Specifically, MMP-9 exhibited significantly higher gelatinolytic activity in La when compared to Lb. Moreover, our data cleverly illustrated the presence and release of MMP-2 and MMP-9 by La and Lb promastigotes, exposing their ability to invade and migrate within a collagen matrix. This pioneering study establishes a compelling correlation between MMP-2 and MMP-9 and their potential role in the dynamics of La and Lb infection. Suggesting their potential as prognostic markers for severe leishmaniasis and promising target molecules for therapeutic interventions, this research opens new avenues for combatting this debilitating parasitic disease.https://doi.org/10.1186/s12866-023-02973-zLeishmaniasisMMP-2MMP-9Collagen matrix |
| spellingShingle | Brenda Furtado Costa Tarcísio Navegante de Queiroz Filho Adeniele Lopes da Cruz Carneiro Aline Semblano Carreira Falcão Maria Sueli da Silva Kataoka João de Jesus Viana Pinheiro Ana Paula Drummond Rodrigues Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes BMC Microbiology Leishmaniasis MMP-2 MMP-9 Collagen matrix |
| title | Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes |
| title_full | Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes |
| title_fullStr | Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes |
| title_full_unstemmed | Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes |
| title_short | Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes |
| title_sort | detection and activity of mmp 2 and mmp 9 in leishmania amazonensis and leishmania braziliensis promastigotes |
| topic | Leishmaniasis MMP-2 MMP-9 Collagen matrix |
| url | https://doi.org/10.1186/s12866-023-02973-z |
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