Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress
The budding yeast Saccharomyces cerevisiae is capable of surviving extreme water loss for a long time. However, less is known about the mechanism of its desiccation tolerance. In this study, we revealed that in an exponential culture, all desiccation tolerant yeast cells were in G1 phase and had con...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shared Science Publishers OG
2021-11-01
|
| Series: | Microbial Cell |
| Subjects: | |
| Online Access: | http://microbialcell.com/researcharticles/2021a-zhang-microbial-cell/ |
| _version_ | 1818950409213968384 |
|---|---|
| author | Zhaojie Zhang Gracie R. Zhang |
| author_facet | Zhaojie Zhang Gracie R. Zhang |
| author_sort | Zhaojie Zhang |
| collection | DOAJ |
| description | The budding yeast Saccharomyces cerevisiae is capable of surviving extreme water loss for a long time. However, less is known about the mechanism of its desiccation tolerance. In this study, we revealed that in an exponential culture, all desiccation tolerant yeast cells were in G1 phase and had condensed chromosomes. These cells share certain features of stationary G0 cells, such as low metabolic level. They were also replicatively young, compared to the desiccation sensitive G1 cells. A similar percentage of chromosome-condensed cells were observed in stationary phase but the condensation level was much higher than that of the log-phase cells. These chromosome-condensed stationary cells were also tolerant to desiccation. However, the majority of the desiccation tolerant cells in stationary phase do not have condensed chromosomes. We speculate that the log-phase cells with condensed chromosome might be a unique feature developed through evolution to survive unpredicted sudden changes of the environment. |
| first_indexed | 2024-12-20T09:18:08Z |
| format | Article |
| id | doaj.art-cfb2a55b536e490fb2c209fb7076bce0 |
| institution | Directory Open Access Journal |
| issn | 2311-2638 |
| language | English |
| last_indexed | 2024-12-20T09:18:08Z |
| publishDate | 2021-11-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj.art-cfb2a55b536e490fb2c209fb7076bce02022-12-21T19:45:22ZengShared Science Publishers OGMicrobial Cell2311-26382021-11-0192425110.15698/mic2022.10.770Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stressZhaojie Zhang0Gracie R. Zhang1Department of Zoology and Physiology, University of Wyoming, Laramie, WY, 82071, USA.Laramie High School, 1710 Boulder Dr. Laramie, WY 82070, USA.The budding yeast Saccharomyces cerevisiae is capable of surviving extreme water loss for a long time. However, less is known about the mechanism of its desiccation tolerance. In this study, we revealed that in an exponential culture, all desiccation tolerant yeast cells were in G1 phase and had condensed chromosomes. These cells share certain features of stationary G0 cells, such as low metabolic level. They were also replicatively young, compared to the desiccation sensitive G1 cells. A similar percentage of chromosome-condensed cells were observed in stationary phase but the condensation level was much higher than that of the log-phase cells. These chromosome-condensed stationary cells were also tolerant to desiccation. However, the majority of the desiccation tolerant cells in stationary phase do not have condensed chromosomes. We speculate that the log-phase cells with condensed chromosome might be a unique feature developed through evolution to survive unpredicted sudden changes of the environment.http://microbialcell.com/researcharticles/2021a-zhang-microbial-cell/agingcell cyclechromosome condensationdesiccation tolerancesaccharomyces cerevisiae |
| spellingShingle | Zhaojie Zhang Gracie R. Zhang Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress Microbial Cell aging cell cycle chromosome condensation desiccation tolerance saccharomyces cerevisiae |
| title | Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress |
| title_full | Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress |
| title_fullStr | Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress |
| title_full_unstemmed | Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress |
| title_short | Chromosome-condensed G1 phase yeast cells are tolerant to desiccation stress |
| title_sort | chromosome condensed g1 phase yeast cells are tolerant to desiccation stress |
| topic | aging cell cycle chromosome condensation desiccation tolerance saccharomyces cerevisiae |
| url | http://microbialcell.com/researcharticles/2021a-zhang-microbial-cell/ |
| work_keys_str_mv | AT zhaojiezhang chromosomecondensedg1phaseyeastcellsaretoleranttodesiccationstress AT gracierzhang chromosomecondensedg1phaseyeastcellsaretoleranttodesiccationstress |