Protein profile of rice (Oryza sativa) seeds
Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2013-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012 |
| _version_ | 1818443646423269376 |
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| author | Yanhua Yang Li Dai Hengchuan Xia Keming Zhu Haijun Liu Keping Chen |
| author_facet | Yanhua Yang Li Dai Hengchuan Xia Keming Zhu Haijun Liu Keping Chen |
| author_sort | Yanhua Yang |
| collection | DOAJ |
| description | Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds. |
| first_indexed | 2024-12-14T19:03:21Z |
| format | Article |
| id | doaj.art-cfb728ffe55c4e44a2d89c5546fb8d52 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-14T19:03:21Z |
| publishDate | 2013-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-cfb728ffe55c4e44a2d89c5546fb8d522022-12-21T22:50:55ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852013-01-01361087092Protein profile of rice (Oryza sativa) seedsYanhua YangLi DaiHengchuan XiaKeming ZhuHaijun LiuKeping ChenSeeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012mass spectrometryproteomic analysisrice seedtwo-dimensional electrophoresis |
| spellingShingle | Yanhua Yang Li Dai Hengchuan Xia Keming Zhu Haijun Liu Keping Chen Protein profile of rice (Oryza sativa) seeds Genetics and Molecular Biology mass spectrometry proteomic analysis rice seed two-dimensional electrophoresis |
| title | Protein profile of rice (Oryza sativa) seeds |
| title_full | Protein profile of rice (Oryza sativa) seeds |
| title_fullStr | Protein profile of rice (Oryza sativa) seeds |
| title_full_unstemmed | Protein profile of rice (Oryza sativa) seeds |
| title_short | Protein profile of rice (Oryza sativa) seeds |
| title_sort | protein profile of rice oryza sativa seeds |
| topic | mass spectrometry proteomic analysis rice seed two-dimensional electrophoresis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012 |
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