Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice
Banhasasim-tang (BHS) is an herbal medicine that has been widely used in East Asia to treat various symptoms associated with upper abdomen swelling. BHS has not been studied previously for neuroinflammation or cognitive disorder. Here, we use a lipopolysaccharide (LPS) model to investigate the effec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/7/2019 |
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| author | You-Chang Oh Yun Hee Jeong Malk Eun Pak Younghoon Go |
| author_facet | You-Chang Oh Yun Hee Jeong Malk Eun Pak Younghoon Go |
| author_sort | You-Chang Oh |
| collection | DOAJ |
| description | Banhasasim-tang (BHS) is an herbal medicine that has been widely used in East Asia to treat various symptoms associated with upper abdomen swelling. BHS has not been studied previously for neuroinflammation or cognitive disorder. Here, we use a lipopolysaccharide (LPS) model to investigate the effects and mechanisms of BHS in neuroinflammation and cognitive impairment of mice. We used a mouse model of LPS-induced cognitive impairment and neuroinflammation and examined whether administration of BHS prevents these deficits via Morris water maze test, passive avoidance test, histopathological analysis, Western blotting, and real-time reverse transcription polymerase chain reaction (RT-qPCR). We found via behavioral tests that BHS treatment effectively prevented LPS-induced memory loss and neuronal damage in mice. Histopathological analysis of mouse brains revealed that BHS inhibited LPS-induced expression of microglial and astrocyte activation markers. Furthermore, BHS inhibits the production of markers related to neurodegeneration, amyloidogenesis, and inflammation, and mRNA expression of inflammatory mediators in mouse brain tissue. Additionally, BHS pretreatment effectively inhibited generation of inflammatory factors and pathways in BV2 microglial cells stimulated by LPS. These observations indicate that BHS is effective in preventing cognitive impairment caused by neuroinflammation and has strong potential as a candidate treatment for neuronal inflammatory diseases. |
| first_indexed | 2024-03-10T18:37:55Z |
| format | Article |
| id | doaj.art-cfb8fd198ca648a988d93446a48b0fc6 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T18:37:55Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-cfb8fd198ca648a988d93446a48b0fc62023-11-20T06:05:49ZengMDPI AGNutrients2072-66432020-07-01127201910.3390/nu12072019Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in MiceYou-Chang Oh0Yun Hee Jeong1Malk Eun Pak2Younghoon Go3Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine, Daegu 41062, KoreaKorean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine, Daegu 41062, KoreaKorean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine, Daegu 41062, KoreaKorean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine, Daegu 41062, KoreaBanhasasim-tang (BHS) is an herbal medicine that has been widely used in East Asia to treat various symptoms associated with upper abdomen swelling. BHS has not been studied previously for neuroinflammation or cognitive disorder. Here, we use a lipopolysaccharide (LPS) model to investigate the effects and mechanisms of BHS in neuroinflammation and cognitive impairment of mice. We used a mouse model of LPS-induced cognitive impairment and neuroinflammation and examined whether administration of BHS prevents these deficits via Morris water maze test, passive avoidance test, histopathological analysis, Western blotting, and real-time reverse transcription polymerase chain reaction (RT-qPCR). We found via behavioral tests that BHS treatment effectively prevented LPS-induced memory loss and neuronal damage in mice. Histopathological analysis of mouse brains revealed that BHS inhibited LPS-induced expression of microglial and astrocyte activation markers. Furthermore, BHS inhibits the production of markers related to neurodegeneration, amyloidogenesis, and inflammation, and mRNA expression of inflammatory mediators in mouse brain tissue. Additionally, BHS pretreatment effectively inhibited generation of inflammatory factors and pathways in BV2 microglial cells stimulated by LPS. These observations indicate that BHS is effective in preventing cognitive impairment caused by neuroinflammation and has strong potential as a candidate treatment for neuronal inflammatory diseases.https://www.mdpi.com/2072-6643/12/7/2019cognitive impairmentneuroinflammationneuronal protectionMorris water mazeMAPKbanhasasim-tang |
| spellingShingle | You-Chang Oh Yun Hee Jeong Malk Eun Pak Younghoon Go Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice Nutrients cognitive impairment neuroinflammation neuronal protection Morris water maze MAPK banhasasim-tang |
| title | Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice |
| title_full | Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice |
| title_fullStr | Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice |
| title_full_unstemmed | Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice |
| title_short | Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice |
| title_sort | banhasasim tang attenuates lipopolysaccharide induced cognitive impairment by suppressing neuroinflammation in mice |
| topic | cognitive impairment neuroinflammation neuronal protection Morris water maze MAPK banhasasim-tang |
| url | https://www.mdpi.com/2072-6643/12/7/2019 |
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