DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response
The immunocompetence and clinical accessibility of dermal tissue offers an appropriate and attractive target for vaccination. We previously demonstrated that pDNA injection into the skin in combination with surface electroporation (SEP), results in rapid and robust expression of the encoded antigen...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-01-01
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| Series: | Molecular Therapy: Methods & Clinical Development |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2329050116301231 |
| _version_ | 1819090787990765568 |
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| author | Trevor RF Smith Katherine Schultheis William B Kiosses Dinah H Amante Janess M Mendoza John C Stone Jay R McCoy Niranjan Y Sardesai Kate E Broderick |
| author_facet | Trevor RF Smith Katherine Schultheis William B Kiosses Dinah H Amante Janess M Mendoza John C Stone Jay R McCoy Niranjan Y Sardesai Kate E Broderick |
| author_sort | Trevor RF Smith |
| collection | DOAJ |
| description | The immunocompetence and clinical accessibility of dermal tissue offers an appropriate and attractive target for vaccination. We previously demonstrated that pDNA injection into the skin in combination with surface electroporation (SEP), results in rapid and robust expression of the encoded antigen in the epidermis. Here, we demonstrate that intradermally EP-enhanced pDNA vaccination results in the rapid induction of a host humoral immune response. In the dermally relevant guinea pig model, we used high-resolution laser scanning confocal microscopy to observe direct dendritic cell (DC) transfections in the epidermis, to determine the migration kinetics of these cells from the epidermal layer into the dermis, and to follow them sequentially to the immediate draining lymph nodes. Furthermore, we delineate the relationship between the migration of directly transfected epidermal DCs and the generation of the host immune response. In summary, these data indicate that direct presentation of antigen to the immune system by DCs through SEP-based in vivo transfection in the epidermis, is related to the generation of a humoral immune response. |
| first_indexed | 2024-12-21T22:29:23Z |
| format | Article |
| id | doaj.art-cfbb15419d64485ab508a8b9d8400c6f |
| institution | Directory Open Access Journal |
| issn | 2329-0501 |
| language | English |
| last_indexed | 2024-12-21T22:29:23Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Methods & Clinical Development |
| spelling | doaj.art-cfbb15419d64485ab508a8b9d8400c6f2022-12-21T18:48:08ZengElsevierMolecular Therapy: Methods & Clinical Development2329-05012014-01-011C10.1038/mtm.2014.54DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune responseTrevor RF Smith0Katherine Schultheis1William B Kiosses2Dinah H Amante3Janess M Mendoza4John C Stone5Jay R McCoy6Niranjan Y Sardesai7Kate E Broderick8Inovio Pharmaceuticals, San Diego, California, USAInovio Pharmaceuticals, San Diego, California, USAThe Scripps Research Institute, Core Microscopy Facility, La Jolla, California, USAInovio Pharmaceuticals, San Diego, California, USAInovio Pharmaceuticals, San Diego, California, USAInovio Pharmaceuticals, San Diego, California, USAInovio Pharmaceuticals, San Diego, California, USAInovio Pharmaceuticals, San Diego, California, USAInovio Pharmaceuticals, San Diego, California, USAThe immunocompetence and clinical accessibility of dermal tissue offers an appropriate and attractive target for vaccination. We previously demonstrated that pDNA injection into the skin in combination with surface electroporation (SEP), results in rapid and robust expression of the encoded antigen in the epidermis. Here, we demonstrate that intradermally EP-enhanced pDNA vaccination results in the rapid induction of a host humoral immune response. In the dermally relevant guinea pig model, we used high-resolution laser scanning confocal microscopy to observe direct dendritic cell (DC) transfections in the epidermis, to determine the migration kinetics of these cells from the epidermal layer into the dermis, and to follow them sequentially to the immediate draining lymph nodes. Furthermore, we delineate the relationship between the migration of directly transfected epidermal DCs and the generation of the host immune response. In summary, these data indicate that direct presentation of antigen to the immune system by DCs through SEP-based in vivo transfection in the epidermis, is related to the generation of a humoral immune response.http://www.sciencedirect.com/science/article/pii/S2329050116301231 |
| spellingShingle | Trevor RF Smith Katherine Schultheis William B Kiosses Dinah H Amante Janess M Mendoza John C Stone Jay R McCoy Niranjan Y Sardesai Kate E Broderick DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response Molecular Therapy: Methods & Clinical Development |
| title | DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response |
| title_full | DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response |
| title_fullStr | DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response |
| title_full_unstemmed | DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response |
| title_short | DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response |
| title_sort | dna vaccination strategy targets epidermal dendritic cells initiating their migration and induction of a host immune response |
| url | http://www.sciencedirect.com/science/article/pii/S2329050116301231 |
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