Bioactivity and Mycochemical Profile of Extracts from Mycelial Cultures of <i>Ganoderma</i> spp.
Fungal mycelium cultures are an alternative to natural sources in order to obtain valuable research materials. They also enable constant control and adaptation of the process, thereby leading to increased biomass growth and accumulation of bioactive metabolites. The present study aims to assess the...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/1/275 |
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| author | Katarzyna Sułkowska-Ziaja Gokhan Zengin Agnieszka Gunia-Krzyżak Justyna Popiół Agnieszka Szewczyk Magdalena Jaszek Jerzy Rogalski Bożena Muszyńska |
| author_facet | Katarzyna Sułkowska-Ziaja Gokhan Zengin Agnieszka Gunia-Krzyżak Justyna Popiół Agnieszka Szewczyk Magdalena Jaszek Jerzy Rogalski Bożena Muszyńska |
| author_sort | Katarzyna Sułkowska-Ziaja |
| collection | DOAJ |
| description | Fungal mycelium cultures are an alternative to natural sources in order to obtain valuable research materials. They also enable constant control and adaptation of the process, thereby leading to increased biomass growth and accumulation of bioactive metabolites. The present study aims to assess the biosynthetic potential of mycelial cultures of six <i>Ganoderma</i> species: <i>G. adspersum</i>, <i>G.</i> <i>applanatum</i>, <i>G. carnosum</i>, <i>G. lucidum</i>, <i>G. pfeifferi</i>, and <i>G. resinaceum</i>. The presence of phenolic acids, amino acids, indole compounds, sterols, and kojic acid in biomass extracts was determined by HPLC. The antioxidant and cytotoxic activities of the extracts and their effects on the inhibition of selected enzymes (tyrosinase and acetylcholinesterase) were also evaluated. The total content of phenolic acids in the extracts ranged from 5.8 (<i>G. carnosum</i>) to 114.07 mg/100 g dry weight (d.w.) (<i>G. pfeifferi</i>). The total content of indole compounds in the extracts ranged from 3.03 (<i>G. carnosum</i>) to 11.56 mg/100 g d.w. (<i>G. lucidum</i>) and that of ergosterol ranged from 28.15 (<i>G. applanatum</i>) to 74.78 mg/100 g d.w. (<i>G. adspersum</i>). Kojic acid was found in the extracts of <i>G. applanatum</i> and <i>G. lucidum</i>. The tested extracts showed significant antioxidant activity. The results suggest that the analyzed mycelial cultures are promising candidates for the development of new dietary supplements or pharmaceutical preparations. |
| first_indexed | 2024-03-10T03:30:17Z |
| format | Article |
| id | doaj.art-cfbf287e845442ca80ac47634064eeba |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:30:17Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cfbf287e845442ca80ac47634064eeba2023-11-23T11:59:16ZengMDPI AGMolecules1420-30492022-01-0127127510.3390/molecules27010275Bioactivity and Mycochemical Profile of Extracts from Mycelial Cultures of <i>Ganoderma</i> spp.Katarzyna Sułkowska-Ziaja0Gokhan Zengin1Agnieszka Gunia-Krzyżak2Justyna Popiół3Agnieszka Szewczyk4Magdalena Jaszek5Jerzy Rogalski6Bożena Muszyńska7Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, PolandPhysiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyDepartment of Bioorganic Chemistry, Chair of Organic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Kraków, PolandDepartment of Pharmaceutical Biochemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Kraków, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, PolandDepartment of Biochemistry and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Biochemistry and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, PolandFungal mycelium cultures are an alternative to natural sources in order to obtain valuable research materials. They also enable constant control and adaptation of the process, thereby leading to increased biomass growth and accumulation of bioactive metabolites. The present study aims to assess the biosynthetic potential of mycelial cultures of six <i>Ganoderma</i> species: <i>G. adspersum</i>, <i>G.</i> <i>applanatum</i>, <i>G. carnosum</i>, <i>G. lucidum</i>, <i>G. pfeifferi</i>, and <i>G. resinaceum</i>. The presence of phenolic acids, amino acids, indole compounds, sterols, and kojic acid in biomass extracts was determined by HPLC. The antioxidant and cytotoxic activities of the extracts and their effects on the inhibition of selected enzymes (tyrosinase and acetylcholinesterase) were also evaluated. The total content of phenolic acids in the extracts ranged from 5.8 (<i>G. carnosum</i>) to 114.07 mg/100 g dry weight (d.w.) (<i>G. pfeifferi</i>). The total content of indole compounds in the extracts ranged from 3.03 (<i>G. carnosum</i>) to 11.56 mg/100 g d.w. (<i>G. lucidum</i>) and that of ergosterol ranged from 28.15 (<i>G. applanatum</i>) to 74.78 mg/100 g d.w. (<i>G. adspersum</i>). Kojic acid was found in the extracts of <i>G. applanatum</i> and <i>G. lucidum</i>. The tested extracts showed significant antioxidant activity. The results suggest that the analyzed mycelial cultures are promising candidates for the development of new dietary supplements or pharmaceutical preparations.https://www.mdpi.com/1420-3049/27/1/275antioxidant activitycholinesterase inhibitioncytotoxic activity<i>Ganoderma</i> spp.indole compoundsmycelial cultures |
| spellingShingle | Katarzyna Sułkowska-Ziaja Gokhan Zengin Agnieszka Gunia-Krzyżak Justyna Popiół Agnieszka Szewczyk Magdalena Jaszek Jerzy Rogalski Bożena Muszyńska Bioactivity and Mycochemical Profile of Extracts from Mycelial Cultures of <i>Ganoderma</i> spp. Molecules antioxidant activity cholinesterase inhibition cytotoxic activity <i>Ganoderma</i> spp. indole compounds mycelial cultures |
| title | Bioactivity and Mycochemical Profile of Extracts from Mycelial Cultures of <i>Ganoderma</i> spp. |
| title_full | Bioactivity and Mycochemical Profile of Extracts from Mycelial Cultures of <i>Ganoderma</i> spp. |
| title_fullStr | Bioactivity and Mycochemical Profile of Extracts from Mycelial Cultures of <i>Ganoderma</i> spp. |
| title_full_unstemmed | Bioactivity and Mycochemical Profile of Extracts from Mycelial Cultures of <i>Ganoderma</i> spp. |
| title_short | Bioactivity and Mycochemical Profile of Extracts from Mycelial Cultures of <i>Ganoderma</i> spp. |
| title_sort | bioactivity and mycochemical profile of extracts from mycelial cultures of i ganoderma i spp |
| topic | antioxidant activity cholinesterase inhibition cytotoxic activity <i>Ganoderma</i> spp. indole compounds mycelial cultures |
| url | https://www.mdpi.com/1420-3049/27/1/275 |
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