Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of <i>Staphylococcus Aureus</i> for Enhanced Photothermal and Antibacterial Wound-Healing Therapy
Photothermal therapy has been widely used in the treatment of bacterial infections. However, the short photothermal effective radius of conventional nano-photothermal agents makes it difficult to achieve effective photothermal antibacterial activity. Therefore, improving composite targeting can sign...
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MDPI AG
2022-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/11/1865 |
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| author | Weiwei Zhang Zhao Kuang Ping Song Wanzhen Li Lin Gui Chuchu Tang Yugui Tao Fei Ge Longbao Zhu |
| author_facet | Weiwei Zhang Zhao Kuang Ping Song Wanzhen Li Lin Gui Chuchu Tang Yugui Tao Fei Ge Longbao Zhu |
| author_sort | Weiwei Zhang |
| collection | DOAJ |
| description | Photothermal therapy has been widely used in the treatment of bacterial infections. However, the short photothermal effective radius of conventional nano-photothermal agents makes it difficult to achieve effective photothermal antibacterial activity. Therefore, improving composite targeting can significantly inhibit bacterial growth. We inhibited the growth of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) by using an extremely low concentration of vancomycin (Van) and applied photothermal therapy with molybdenum disulfide (MoS<sub>2</sub>). This simple method used chitosan (CS) to synthesize fluorescein 5(6)-isothiocyanate (FITC)-labeled and Van-loaded MoS<sub>2</sub>-nanosheet hydrogels (MoS<sub>2</sub>-Van-FITC@CS). After modifying the surface, an extremely low concentration of Van could inhibit bacterial growth by trapping bacteria synergistically with the photothermal effects of MoS<sub>2</sub>, while FITC labeled bacteria and chitosan hydrogels promoted wound healing. The results showed that MoS<sub>2</sub>-Van-FITC@CS nanosheets had a thickness of approximately 30 nm, indicating the successful synthesis of the nanosheets. The vitro antibacterial results showed that MoS<sub>2</sub>-Van-FITC with near-infrared irradiation significantly inhibited <i>S. aureus</i> growth, reaching an inhibition rate of 94.5% at nanoparticle concentrations of up to 100 µg/mL. Furthermore, MoS<sub>2</sub>-Van-FITC@CS could exert a healing effect on wounds in mice. Our results demonstrate that MoS<sub>2</sub>-Van-FITC@CS is biocompatible and can be used as a wound-healing agent. |
| first_indexed | 2024-03-10T01:01:57Z |
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| language | English |
| last_indexed | 2024-03-10T01:01:57Z |
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| series | Nanomaterials |
| spelling | doaj.art-cfc2a941410247608c1901d2d81425572023-11-23T14:33:30ZengMDPI AGNanomaterials2079-49912022-05-011211186510.3390/nano12111865Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of <i>Staphylococcus Aureus</i> for Enhanced Photothermal and Antibacterial Wound-Healing TherapyWeiwei Zhang0Zhao Kuang1Ping Song2Wanzhen Li3Lin Gui4Chuchu Tang5Yugui Tao6Fei Ge7Longbao Zhu8School of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaSchool of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaSchool of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaSchool of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaDepartment of Microbiology and Immunology, Wannan Medical College, Wuhu 241002, ChinaSchool of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaSchool of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaSchool of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaSchool of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaPhotothermal therapy has been widely used in the treatment of bacterial infections. However, the short photothermal effective radius of conventional nano-photothermal agents makes it difficult to achieve effective photothermal antibacterial activity. Therefore, improving composite targeting can significantly inhibit bacterial growth. We inhibited the growth of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) by using an extremely low concentration of vancomycin (Van) and applied photothermal therapy with molybdenum disulfide (MoS<sub>2</sub>). This simple method used chitosan (CS) to synthesize fluorescein 5(6)-isothiocyanate (FITC)-labeled and Van-loaded MoS<sub>2</sub>-nanosheet hydrogels (MoS<sub>2</sub>-Van-FITC@CS). After modifying the surface, an extremely low concentration of Van could inhibit bacterial growth by trapping bacteria synergistically with the photothermal effects of MoS<sub>2</sub>, while FITC labeled bacteria and chitosan hydrogels promoted wound healing. The results showed that MoS<sub>2</sub>-Van-FITC@CS nanosheets had a thickness of approximately 30 nm, indicating the successful synthesis of the nanosheets. The vitro antibacterial results showed that MoS<sub>2</sub>-Van-FITC with near-infrared irradiation significantly inhibited <i>S. aureus</i> growth, reaching an inhibition rate of 94.5% at nanoparticle concentrations of up to 100 µg/mL. Furthermore, MoS<sub>2</sub>-Van-FITC@CS could exert a healing effect on wounds in mice. Our results demonstrate that MoS<sub>2</sub>-Van-FITC@CS is biocompatible and can be used as a wound-healing agent.https://www.mdpi.com/2079-4991/12/11/1865MoS<sub>2</sub> nanosheetantibacterial activityphotothermal therapywound-healing |
| spellingShingle | Weiwei Zhang Zhao Kuang Ping Song Wanzhen Li Lin Gui Chuchu Tang Yugui Tao Fei Ge Longbao Zhu Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of <i>Staphylococcus Aureus</i> for Enhanced Photothermal and Antibacterial Wound-Healing Therapy Nanomaterials MoS<sub>2</sub> nanosheet antibacterial activity photothermal therapy wound-healing |
| title | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of <i>Staphylococcus Aureus</i> for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
| title_full | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of <i>Staphylococcus Aureus</i> for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
| title_fullStr | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of <i>Staphylococcus Aureus</i> for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
| title_full_unstemmed | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of <i>Staphylococcus Aureus</i> for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
| title_short | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of <i>Staphylococcus Aureus</i> for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
| title_sort | synthesis of a two dimensional molybdenum disulfide nanosheet and ultrasensitive trapping of i staphylococcus aureus i for enhanced photothermal and antibacterial wound healing therapy |
| topic | MoS<sub>2</sub> nanosheet antibacterial activity photothermal therapy wound-healing |
| url | https://www.mdpi.com/2079-4991/12/11/1865 |
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