Rapid SERS Detection of Botulinum Neurotoxin Type A
Surface-enhanced Raman scattering (SERS) is a powerful technique for decoding of 2-5-component mixes of analytes. Low concentrations of analytes and complex biological media are usually non-decodable with SERS. Recognition molecules, such as antibodies and aptamers, provide an opportunity for a spec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/18/2531 |
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| author | Alexei Subekin Rugiya Alieva Vladimir Kukushkin Ilya Oleynikov Elena Zavyalova |
| author_facet | Alexei Subekin Rugiya Alieva Vladimir Kukushkin Ilya Oleynikov Elena Zavyalova |
| author_sort | Alexei Subekin |
| collection | DOAJ |
| description | Surface-enhanced Raman scattering (SERS) is a powerful technique for decoding of 2-5-component mixes of analytes. Low concentrations of analytes and complex biological media are usually non-decodable with SERS. Recognition molecules, such as antibodies and aptamers, provide an opportunity for a specific binding of ultra-low contents of analyte dissolved in complex biological media. Different approaches have been proposed to provide changes in SERS intensity of an external label upon binding of ultra-low contents of the analytes. In this paper, we propose a SERS-based sensor for the rapid and sensitive detection of botulinum toxin type A. The silver nanoisland SERS substrate was functionalized using an aptamer conjugated with a Raman label. The binding of the target affects the orientation of the label, providing changes in an analytical signal. This trick allowed detecting botulinum toxin type A in a one-stage manner without additional staining with a monotonous dose dependence and a limit of detection of 2.4 ng/mL. The proposed sensor architecture is consistent with the multiarray detection systems for multiplex analyses. |
| first_indexed | 2024-03-10T22:22:36Z |
| format | Article |
| id | doaj.art-cfc2c92e5d6c40bab35b51d623aff87a |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T22:22:36Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-cfc2c92e5d6c40bab35b51d623aff87a2023-11-19T12:14:17ZengMDPI AGNanomaterials2079-49912023-09-011318253110.3390/nano13182531Rapid SERS Detection of Botulinum Neurotoxin Type AAlexei Subekin0Rugiya Alieva1Vladimir Kukushkin2Ilya Oleynikov3Elena Zavyalova4Moscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaOsipyan Institute of Solid State Physics of the Russian Academy of Science, 142432 Chernogolovka, RussiaA.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, RussiaMoscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaSurface-enhanced Raman scattering (SERS) is a powerful technique for decoding of 2-5-component mixes of analytes. Low concentrations of analytes and complex biological media are usually non-decodable with SERS. Recognition molecules, such as antibodies and aptamers, provide an opportunity for a specific binding of ultra-low contents of analyte dissolved in complex biological media. Different approaches have been proposed to provide changes in SERS intensity of an external label upon binding of ultra-low contents of the analytes. In this paper, we propose a SERS-based sensor for the rapid and sensitive detection of botulinum toxin type A. The silver nanoisland SERS substrate was functionalized using an aptamer conjugated with a Raman label. The binding of the target affects the orientation of the label, providing changes in an analytical signal. This trick allowed detecting botulinum toxin type A in a one-stage manner without additional staining with a monotonous dose dependence and a limit of detection of 2.4 ng/mL. The proposed sensor architecture is consistent with the multiarray detection systems for multiplex analyses.https://www.mdpi.com/2079-4991/13/18/2531aptameraptasensorbiosensorbotulinum neurotoxinSERS |
| spellingShingle | Alexei Subekin Rugiya Alieva Vladimir Kukushkin Ilya Oleynikov Elena Zavyalova Rapid SERS Detection of Botulinum Neurotoxin Type A Nanomaterials aptamer aptasensor biosensor botulinum neurotoxin SERS |
| title | Rapid SERS Detection of Botulinum Neurotoxin Type A |
| title_full | Rapid SERS Detection of Botulinum Neurotoxin Type A |
| title_fullStr | Rapid SERS Detection of Botulinum Neurotoxin Type A |
| title_full_unstemmed | Rapid SERS Detection of Botulinum Neurotoxin Type A |
| title_short | Rapid SERS Detection of Botulinum Neurotoxin Type A |
| title_sort | rapid sers detection of botulinum neurotoxin type a |
| topic | aptamer aptasensor biosensor botulinum neurotoxin SERS |
| url | https://www.mdpi.com/2079-4991/13/18/2531 |
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