Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coating
Abstract A contiguous carbonate‐rich hydroxyapatite microcoating in a microfluidic device represents a substrate that has chemical and structural similarity to bone mineral. The present work describes a low‐temperature method to deposit a carbonate‐rich hydroxyapatite microcoating on a glass slide a...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2022-09-01
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| Series: | Nano Select |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/nano.202200102 |
| _version_ | 1818480075085971456 |
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| author | Florence H. Y. Lui Liangcheng Xu Pierrette Michaux Joanna Biazik Gregory F. S. Harm Rema A. Oliver Pramod Koshy William R. Walsh Ralph J. Mobbs Tara C. Brennan‐Speranza Yu Wang Lidan You Charles C. Sorrell |
| author_facet | Florence H. Y. Lui Liangcheng Xu Pierrette Michaux Joanna Biazik Gregory F. S. Harm Rema A. Oliver Pramod Koshy William R. Walsh Ralph J. Mobbs Tara C. Brennan‐Speranza Yu Wang Lidan You Charles C. Sorrell |
| author_sort | Florence H. Y. Lui |
| collection | DOAJ |
| description | Abstract A contiguous carbonate‐rich hydroxyapatite microcoating in a microfluidic device represents a substrate that has chemical and structural similarity to bone mineral. The present work describes a low‐temperature method to deposit a carbonate‐rich hydroxyapatite microcoating on a glass slide and its incorporation within the microchannels of a microfluidic device. A glass slide is covered/masked with polypropylene‐based tape and CaCO3 nanoparticles are deposited on exposed areas by convective self assembly. The precursor CaCO3 is converted to carbonate‐rich hydroxyapatite by dissolution‐recrystallization in phosphate‐buffered saline. The microcoating is aligned/incorporated within a microchannel when the underlying glass is bonded to a polydimethylsiloxane structure with the device layout. X‐ray diffraction, laser Raman microspectroscopy, and X‐ray photoelectron spectroscopy indicate that the microcoating was comprised of carbonate‐rich hydroxyapatite. Scanning electron microscopy and 3D laser confocal microscopy showed that was comprised of nanocrystalline rod‐like clusters that collectively exhibit a thickness of ∼20 µm. Monocultures/cocultures of osteoblast‐lineage (MC3T3‐E1, MG63) and preosteoclast‐lineage (RAW 264.7) cells were performed. Osteoblast‐lineage cells adhered to the microcoating and deposited an extracellular matrix of collagen fibrils and mineral accretions. Mineralization was detected in/near the inlet wells. The microcoating is analogous to bone mineral and could be applied to various layouts and mineral systems. |
| first_indexed | 2024-12-10T11:18:25Z |
| format | Article |
| id | doaj.art-cfc2e42352154088b1fa5b177fb1095a |
| institution | Directory Open Access Journal |
| issn | 2688-4011 |
| language | English |
| last_indexed | 2024-12-10T11:18:25Z |
| publishDate | 2022-09-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Nano Select |
| spelling | doaj.art-cfc2e42352154088b1fa5b177fb1095a2022-12-22T01:51:04ZengWiley-VCHNano Select2688-40112022-09-01391321133610.1002/nano.202200102Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coatingFlorence H. Y. Lui0Liangcheng Xu1Pierrette Michaux2Joanna Biazik3Gregory F. S. Harm4Rema A. Oliver5Pramod Koshy6William R. Walsh7Ralph J. Mobbs8Tara C. Brennan‐Speranza9Yu Wang10Lidan You11Charles C. Sorrell12School of Materials Science and Engineering UNSW Sydney Sydney New South Wales AustraliaInstitute of Biomedical Engineering University of Toronto Toronto Ontario CanadaAustralian National Fabrication Facility (NSW Node) School of Physics UNSW Sydney Sydney New South Wales AustraliaMark Wainwright Cell Culture Facility UNSW Sydney Sydney New South Wales AustraliaMark Wainwright Cell Culture Facility UNSW Sydney Sydney New South Wales AustraliaSurgical & Orthopaedic Research Laboratories (SORL) Prince of Wales Clinical School UNSW Sydney Sydney New South Wales AustraliaSchool of Materials Science and Engineering UNSW Sydney Sydney New South Wales AustraliaSurgical & Orthopaedic Research Laboratories (SORL) Prince of Wales Clinical School UNSW Sydney Sydney New South Wales AustraliaPrince of Wales Hospital School of Medicine UNSW Sydney Sydney New South Wales AustraliaSchool of Medical Sciences The University of Sydney Camperdown New South Wales AustraliaMark Wainwright Analytical Centre UNSW Sydney Sydney New South Wales AustraliaInstitute of Biomedical Engineering University of Toronto Toronto Ontario CanadaSchool of Materials Science and Engineering UNSW Sydney Sydney New South Wales AustraliaAbstract A contiguous carbonate‐rich hydroxyapatite microcoating in a microfluidic device represents a substrate that has chemical and structural similarity to bone mineral. The present work describes a low‐temperature method to deposit a carbonate‐rich hydroxyapatite microcoating on a glass slide and its incorporation within the microchannels of a microfluidic device. A glass slide is covered/masked with polypropylene‐based tape and CaCO3 nanoparticles are deposited on exposed areas by convective self assembly. The precursor CaCO3 is converted to carbonate‐rich hydroxyapatite by dissolution‐recrystallization in phosphate‐buffered saline. The microcoating is aligned/incorporated within a microchannel when the underlying glass is bonded to a polydimethylsiloxane structure with the device layout. X‐ray diffraction, laser Raman microspectroscopy, and X‐ray photoelectron spectroscopy indicate that the microcoating was comprised of carbonate‐rich hydroxyapatite. Scanning electron microscopy and 3D laser confocal microscopy showed that was comprised of nanocrystalline rod‐like clusters that collectively exhibit a thickness of ∼20 µm. Monocultures/cocultures of osteoblast‐lineage (MC3T3‐E1, MG63) and preosteoclast‐lineage (RAW 264.7) cells were performed. Osteoblast‐lineage cells adhered to the microcoating and deposited an extracellular matrix of collagen fibrils and mineral accretions. Mineralization was detected in/near the inlet wells. The microcoating is analogous to bone mineral and could be applied to various layouts and mineral systems.https://doi.org/10.1002/nano.202200102bone‐on‐a‐chipcarbonate hydroxyapatitemicrofluidicmineralizationosteoblast |
| spellingShingle | Florence H. Y. Lui Liangcheng Xu Pierrette Michaux Joanna Biazik Gregory F. S. Harm Rema A. Oliver Pramod Koshy William R. Walsh Ralph J. Mobbs Tara C. Brennan‐Speranza Yu Wang Lidan You Charles C. Sorrell Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coating Nano Select bone‐on‐a‐chip carbonate hydroxyapatite microfluidic mineralization osteoblast |
| title | Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coating |
| title_full | Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coating |
| title_fullStr | Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coating |
| title_full_unstemmed | Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coating |
| title_short | Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coating |
| title_sort | microfluidic device with a carbonate rich hydroxyapatite micro coating |
| topic | bone‐on‐a‐chip carbonate hydroxyapatite microfluidic mineralization osteoblast |
| url | https://doi.org/10.1002/nano.202200102 |
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