Responses of gut microbiota in crocodile lizards (Shinisaurus crocodilurus) to changes in temperature
The gut microbiota plays an essential role in maintaining the health and fitness of the host organism. As a critical environmental variable, temperature exerts significant effects on animal survival and reproduction. Elevated temperatures can influence the composition and function of the animal gut...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1263917/full |
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| author | Zhengzhong Lin Zhengzhong Lin Mingxian He Chunying Zhong Yuhui Li Yuhui Li Sanqi Tang Sanqi Tang Xindan Kang Xindan Kang Zhengjun Wu Zhengjun Wu |
| author_facet | Zhengzhong Lin Zhengzhong Lin Mingxian He Chunying Zhong Yuhui Li Yuhui Li Sanqi Tang Sanqi Tang Xindan Kang Xindan Kang Zhengjun Wu Zhengjun Wu |
| author_sort | Zhengzhong Lin |
| collection | DOAJ |
| description | The gut microbiota plays an essential role in maintaining the health and fitness of the host organism. As a critical environmental variable, temperature exerts significant effects on animal survival and reproduction. Elevated temperatures can influence the composition and function of the animal gut microbiota, which may have potentially detrimental effects on the host. The crocodile lizard (Shinisaurus crocodilurus) is an ancient and currently endangered reptile species due to human hunting and habitat destruction. Given the predicted shifts in global temperatures in the next century, it is important to understand how warming affects the gut microbiota of these vulnerable lizards, which remains unclear. To determine how the microbial communities change in crocodile lizards in response to warming, we analyzed the gut microbiota under five temperature conditions (22°C, 24°C, 26°C, 28°C, and 30°C) using 16S rRNA high-throughput sequencing. Results showed that the dominant phyla, Proteobacteria and Bacteroidetes, in gut microbiota were not significantly affected by temperature variations, but increasing temperature altered the structure and increased the community richness of the gut microbiota. In addition, warming changed the abundance of Pseudomonas aeruginosa and Actinobacteria, which may have negative effects on the physiological health of the crocodile lizards. Functional prediction analysis demonstrated that the functional pathways enriched in crocodile lizards were mainly related to metabolism, with no significant differences observed in these pathways at KEGG pathway level 1 after warming. These results provide valuable insights into the ecological adaptations and regulatory mechanisms employed by crocodile lizards in response to warming, which may be of benefit for their conservation. |
| first_indexed | 2024-03-11T07:05:58Z |
| format | Article |
| id | doaj.art-cfc5c66ba74c4231b388071fbfc71886 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T07:05:58Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cfc5c66ba74c4231b388071fbfc718862023-11-17T08:56:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-11-011410.3389/fmicb.2023.12639171263917Responses of gut microbiota in crocodile lizards (Shinisaurus crocodilurus) to changes in temperatureZhengzhong Lin0Zhengzhong Lin1Mingxian He2Chunying Zhong3Yuhui Li4Yuhui Li5Sanqi Tang6Sanqi Tang7Xindan Kang8Xindan Kang9Zhengjun Wu10Zhengjun Wu11Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin, ChinaCollege of Food and Biochemical Engineering, Guangxi Science and Technology Normal University, Guangxi, ChinaCollege of Vocational and Technical Education, Guangxi Science and Technology Normal University, Guangxi, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin, ChinaThe gut microbiota plays an essential role in maintaining the health and fitness of the host organism. As a critical environmental variable, temperature exerts significant effects on animal survival and reproduction. Elevated temperatures can influence the composition and function of the animal gut microbiota, which may have potentially detrimental effects on the host. The crocodile lizard (Shinisaurus crocodilurus) is an ancient and currently endangered reptile species due to human hunting and habitat destruction. Given the predicted shifts in global temperatures in the next century, it is important to understand how warming affects the gut microbiota of these vulnerable lizards, which remains unclear. To determine how the microbial communities change in crocodile lizards in response to warming, we analyzed the gut microbiota under five temperature conditions (22°C, 24°C, 26°C, 28°C, and 30°C) using 16S rRNA high-throughput sequencing. Results showed that the dominant phyla, Proteobacteria and Bacteroidetes, in gut microbiota were not significantly affected by temperature variations, but increasing temperature altered the structure and increased the community richness of the gut microbiota. In addition, warming changed the abundance of Pseudomonas aeruginosa and Actinobacteria, which may have negative effects on the physiological health of the crocodile lizards. Functional prediction analysis demonstrated that the functional pathways enriched in crocodile lizards were mainly related to metabolism, with no significant differences observed in these pathways at KEGG pathway level 1 after warming. These results provide valuable insights into the ecological adaptations and regulatory mechanisms employed by crocodile lizards in response to warming, which may be of benefit for their conservation.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1263917/fullShinisaurus crocodilurustemperaturegut microbiotaresponses16S rRNA high-throughput sequencing |
| spellingShingle | Zhengzhong Lin Zhengzhong Lin Mingxian He Chunying Zhong Yuhui Li Yuhui Li Sanqi Tang Sanqi Tang Xindan Kang Xindan Kang Zhengjun Wu Zhengjun Wu Responses of gut microbiota in crocodile lizards (Shinisaurus crocodilurus) to changes in temperature Frontiers in Microbiology Shinisaurus crocodilurus temperature gut microbiota responses 16S rRNA high-throughput sequencing |
| title | Responses of gut microbiota in crocodile lizards (Shinisaurus crocodilurus) to changes in temperature |
| title_full | Responses of gut microbiota in crocodile lizards (Shinisaurus crocodilurus) to changes in temperature |
| title_fullStr | Responses of gut microbiota in crocodile lizards (Shinisaurus crocodilurus) to changes in temperature |
| title_full_unstemmed | Responses of gut microbiota in crocodile lizards (Shinisaurus crocodilurus) to changes in temperature |
| title_short | Responses of gut microbiota in crocodile lizards (Shinisaurus crocodilurus) to changes in temperature |
| title_sort | responses of gut microbiota in crocodile lizards shinisaurus crocodilurus to changes in temperature |
| topic | Shinisaurus crocodilurus temperature gut microbiota responses 16S rRNA high-throughput sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1263917/full |
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