MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages
Small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. We investigated the dynamics of expression of MTS1338, a small non-coding RNA of Mycobacterium tuberculosis, in the mouse model in vivo, regulation of its expression in the infected macrophage...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2019.00405/full |
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| author | Elena G. Salina Artem Grigorov Yulia Skvortsova Konstantin Majorov Oksana Bychenko Albina Ostrik Nadezhda Logunova Dmitriy Ignatov Arseny Kaprelyants Alexander Apt Tatyana Azhikina |
| author_facet | Elena G. Salina Artem Grigorov Yulia Skvortsova Konstantin Majorov Oksana Bychenko Albina Ostrik Nadezhda Logunova Dmitriy Ignatov Arseny Kaprelyants Alexander Apt Tatyana Azhikina |
| author_sort | Elena G. Salina |
| collection | DOAJ |
| description | Small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. We investigated the dynamics of expression of MTS1338, a small non-coding RNA of Mycobacterium tuberculosis, in the mouse model in vivo, regulation of its expression in the infected macrophages, and the consequences of its overexpression in bacterial cultures. Here we demonstrate that MTS1338 significantly contributes to host-pathogen interactions. Activation of the host immune system triggered NO-inducible up-regulation of MTS1338 in macrophage-engulfed mycobacteria. Constitutive overexpression of MTS1338 in cultured mycobacteria improved their survival in vitro under low pH conditions. MTS1338 up-regulation launched a spectrum of shifts in the transcriptome profile similar to those reported for M. tuberculosis adaptation to hostile intra-macrophage environment. Using the RNA-seq approach, we demonstrate that gene expression changes accompanying MTS1338 overexpression indicate reduction in translational activity and bacterial growth. These changes indicate mycobacteria entering the dormant state. Taken together, our results suggest a direct involvement of this sRNA in the interplay between mycobacteria and the host immune system during infectious process. |
| first_indexed | 2024-12-21T21:19:45Z |
| format | Article |
| id | doaj.art-cfc6724d335d456ca90af3f7ffc1c6ee |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-21T21:19:45Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-cfc6724d335d456ca90af3f7ffc1c6ee2022-12-21T18:49:55ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-11-01910.3389/fcimb.2019.00405501725MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host MacrophagesElena G. Salina0Artem Grigorov1Yulia Skvortsova2Konstantin Majorov3Oksana Bychenko4Albina Ostrik5Nadezhda Logunova6Dmitriy Ignatov7Arseny Kaprelyants8Alexander Apt9Tatyana Azhikina10Laboratory of Biochemistry of Stresses in Microorganisms, Bach Institute of Biochemistry, Research Center of Biotechnology, Moscow, RussiaLaboratory of Regulatory Transcriptomics, Department of Genomics and Postgenomic Technologies, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, RussiaLaboratory of Regulatory Transcriptomics, Department of Genomics and Postgenomic Technologies, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, RussiaLaboratory for Immunogenetics, Department of Immunology, Central Institute for Tuberculosis, Moscow, RussiaLaboratory of Regulatory Transcriptomics, Department of Genomics and Postgenomic Technologies, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, RussiaLaboratory of Biochemistry of Stresses in Microorganisms, Bach Institute of Biochemistry, Research Center of Biotechnology, Moscow, RussiaLaboratory for Immunogenetics, Department of Immunology, Central Institute for Tuberculosis, Moscow, RussiaLaboratory of Regulatory Transcriptomics, Department of Genomics and Postgenomic Technologies, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, RussiaLaboratory of Biochemistry of Stresses in Microorganisms, Bach Institute of Biochemistry, Research Center of Biotechnology, Moscow, RussiaLaboratory for Immunogenetics, Department of Immunology, Central Institute for Tuberculosis, Moscow, RussiaLaboratory of Regulatory Transcriptomics, Department of Genomics and Postgenomic Technologies, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, RussiaSmall non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. We investigated the dynamics of expression of MTS1338, a small non-coding RNA of Mycobacterium tuberculosis, in the mouse model in vivo, regulation of its expression in the infected macrophages, and the consequences of its overexpression in bacterial cultures. Here we demonstrate that MTS1338 significantly contributes to host-pathogen interactions. Activation of the host immune system triggered NO-inducible up-regulation of MTS1338 in macrophage-engulfed mycobacteria. Constitutive overexpression of MTS1338 in cultured mycobacteria improved their survival in vitro under low pH conditions. MTS1338 up-regulation launched a spectrum of shifts in the transcriptome profile similar to those reported for M. tuberculosis adaptation to hostile intra-macrophage environment. Using the RNA-seq approach, we demonstrate that gene expression changes accompanying MTS1338 overexpression indicate reduction in translational activity and bacterial growth. These changes indicate mycobacteria entering the dormant state. Taken together, our results suggest a direct involvement of this sRNA in the interplay between mycobacteria and the host immune system during infectious process.https://www.frontiersin.org/article/10.3389/fcimb.2019.00405/fullMycobacterium tuberculosistuberculosissmall RNA MTS1338RNA-seqinfection |
| spellingShingle | Elena G. Salina Artem Grigorov Yulia Skvortsova Konstantin Majorov Oksana Bychenko Albina Ostrik Nadezhda Logunova Dmitriy Ignatov Arseny Kaprelyants Alexander Apt Tatyana Azhikina MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages Frontiers in Cellular and Infection Microbiology Mycobacterium tuberculosis tuberculosis small RNA MTS1338 RNA-seq infection |
| title | MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages |
| title_full | MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages |
| title_fullStr | MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages |
| title_full_unstemmed | MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages |
| title_short | MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages |
| title_sort | mts1338 a small mycobacterium tuberculosis rna regulates transcriptional shifts consistent with bacterial adaptation for entering into dormancy and survival within host macrophages |
| topic | Mycobacterium tuberculosis tuberculosis small RNA MTS1338 RNA-seq infection |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2019.00405/full |
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