Physiological response and molecular mechanisms against UV-B radiation in Brachionus asplanchnoidis (Rotifera)
Ultraviolet B (UV-B, 280–320 nm) radiation is a major environmental stressor for aquatic organisms on Earth's surface. Its effects on biological systems are well known, but the mechanisms by which organisms respond and adapt to UV-B radiation are still being explored. In this study, we investig...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323008230 |
| _version_ | 1797744159065374720 |
|---|---|
| author | Dongqi Kan Yu Zhang Jiancheng Zeng Hairong Lian Lei Feng Yifan Feng Xiaojie Liu Cui Han Jiaxin Yang |
| author_facet | Dongqi Kan Yu Zhang Jiancheng Zeng Hairong Lian Lei Feng Yifan Feng Xiaojie Liu Cui Han Jiaxin Yang |
| author_sort | Dongqi Kan |
| collection | DOAJ |
| description | Ultraviolet B (UV-B, 280–320 nm) radiation is a major environmental stressor for aquatic organisms on Earth's surface. Its effects on biological systems are well known, but the mechanisms by which organisms respond and adapt to UV-B radiation are still being explored. In this study, we investigated the effects of UV-B radiation on the monogonont rotifer Brachionus asplanchnoidis, focusing on physiological parameters, antioxidant systems, DNA damage, and DNA repair-related molecular mechanism. Our results showed that the LD50 was at 28.53 kJ/m2, indicating strong tolerance to UV-B. However, UV-B radiation caused adverse effects on growth and reproduction, with shortened reproductive period and longevity, decreased fecundity and hatchability, and inhibition of population growth. Biochemical analyses revealed severe oxidative damage and lipid peroxidation, with increased ROS and MDA levels. Activities of antioxidant enzymes were highly induced at low doses but decreased at high doses. DNA damage also occurred in UV-B-exposed rotifers. Furthermore, selected DNA repair-related genes were up-regulated in a dose-dependent manner. These findings provide a comprehensive understanding of the effects of UV-B radiation on rotifers and highlight the importance of considering both ecological and molecular responses in assessing the impact of UV-B radiation on aquatic organisms. |
| first_indexed | 2024-03-12T15:05:45Z |
| format | Article |
| id | doaj.art-cfc69204d9244d6790b2e4769ef48d8a |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-12T15:05:45Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-cfc69204d9244d6790b2e4769ef48d8a2023-08-13T04:52:04ZengElsevierEcotoxicology and Environmental Safety0147-65132023-09-01262115319Physiological response and molecular mechanisms against UV-B radiation in Brachionus asplanchnoidis (Rotifera)Dongqi Kan0Yu Zhang1Jiancheng Zeng2Hairong Lian3Lei Feng4Yifan Feng5Xiaojie Liu6Cui Han7Jiaxin Yang8School of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of ChinaSchool of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of ChinaSchool of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of ChinaSchool of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of China; School of Ecology and Environment, Anhui Normal University, Wuhu, Anhui Province 241002, People's Republic of ChinaSchool of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of ChinaSchool of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of ChinaSchool of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of ChinaSchool of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of ChinaSchool of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of China; Corresponding author.Ultraviolet B (UV-B, 280–320 nm) radiation is a major environmental stressor for aquatic organisms on Earth's surface. Its effects on biological systems are well known, but the mechanisms by which organisms respond and adapt to UV-B radiation are still being explored. In this study, we investigated the effects of UV-B radiation on the monogonont rotifer Brachionus asplanchnoidis, focusing on physiological parameters, antioxidant systems, DNA damage, and DNA repair-related molecular mechanism. Our results showed that the LD50 was at 28.53 kJ/m2, indicating strong tolerance to UV-B. However, UV-B radiation caused adverse effects on growth and reproduction, with shortened reproductive period and longevity, decreased fecundity and hatchability, and inhibition of population growth. Biochemical analyses revealed severe oxidative damage and lipid peroxidation, with increased ROS and MDA levels. Activities of antioxidant enzymes were highly induced at low doses but decreased at high doses. DNA damage also occurred in UV-B-exposed rotifers. Furthermore, selected DNA repair-related genes were up-regulated in a dose-dependent manner. These findings provide a comprehensive understanding of the effects of UV-B radiation on rotifers and highlight the importance of considering both ecological and molecular responses in assessing the impact of UV-B radiation on aquatic organisms.http://www.sciencedirect.com/science/article/pii/S0147651323008230UV-B radiationB. asplanchnoidisLife history parametersPopulation growth rateOxidative stressDNA damage |
| spellingShingle | Dongqi Kan Yu Zhang Jiancheng Zeng Hairong Lian Lei Feng Yifan Feng Xiaojie Liu Cui Han Jiaxin Yang Physiological response and molecular mechanisms against UV-B radiation in Brachionus asplanchnoidis (Rotifera) Ecotoxicology and Environmental Safety UV-B radiation B. asplanchnoidis Life history parameters Population growth rate Oxidative stress DNA damage |
| title | Physiological response and molecular mechanisms against UV-B radiation in Brachionus asplanchnoidis (Rotifera) |
| title_full | Physiological response and molecular mechanisms against UV-B radiation in Brachionus asplanchnoidis (Rotifera) |
| title_fullStr | Physiological response and molecular mechanisms against UV-B radiation in Brachionus asplanchnoidis (Rotifera) |
| title_full_unstemmed | Physiological response and molecular mechanisms against UV-B radiation in Brachionus asplanchnoidis (Rotifera) |
| title_short | Physiological response and molecular mechanisms against UV-B radiation in Brachionus asplanchnoidis (Rotifera) |
| title_sort | physiological response and molecular mechanisms against uv b radiation in brachionus asplanchnoidis rotifera |
| topic | UV-B radiation B. asplanchnoidis Life history parameters Population growth rate Oxidative stress DNA damage |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323008230 |
| work_keys_str_mv | AT dongqikan physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera AT yuzhang physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera AT jianchengzeng physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera AT haironglian physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera AT leifeng physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera AT yifanfeng physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera AT xiaojieliu physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera AT cuihan physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera AT jiaxinyang physiologicalresponseandmolecularmechanismsagainstuvbradiationinbrachionusasplanchnoidisrotifera |