New Trend in Toxicological Screening Using Volumetric Absorptive Microsampling (VAMS) and High-Resolution Mass Spectrometry (HR/MS) Combination
In toxicology, screenings are routinely performed using chromatographic methods coupled to detection systems such as high-resolution mass spectrometry (HR/MS). The increase in specificity and sensitivity of HRMS is responsible for the development of methods for alternative samples such as Volumetric...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/8/3466 |
| _version_ | 1797604124616818688 |
|---|---|
| author | Pascal Houzé Ilona Borowski Eugénie Bito Romain Magny Athina Morcos Sebastian Voicu Bruno Mégarbane Laurence Labat |
| author_facet | Pascal Houzé Ilona Borowski Eugénie Bito Romain Magny Athina Morcos Sebastian Voicu Bruno Mégarbane Laurence Labat |
| author_sort | Pascal Houzé |
| collection | DOAJ |
| description | In toxicology, screenings are routinely performed using chromatographic methods coupled to detection systems such as high-resolution mass spectrometry (HR/MS). The increase in specificity and sensitivity of HRMS is responsible for the development of methods for alternative samples such as Volumetric Adsorptive Micro-Sampling. Whole blood overloaded with 90 drugs was sampled with 20 µL Mitra<sup>TM</sup> to optimize the pre-analytical step as well as to determine the identification limits of drugs. Elution of chemicals was carried out in a solvent mixture through agitation and sonication. After dissolution, 10 μL was injected into the chromatographic system coupled to the Orbitrap<sup>TM</sup> HR/MS. Compounds were confirmed against the laboratory library. The clinical feasibility was assessed in fifteen poisoned patients using the simultaneous sampling of plasma, whole blood and Mitra<sup>TM</sup>. The optimized extraction procedure allowed us to confirm 87 compounds out of the 90 present in the spiked whole blood. Cannabis derivatives were not detected. For 82.2% of the investigated drugs, the identification limits were below 12.5 ng·mL<sup>−1</sup>, with the extraction yields ranging from 80.6 to 108.7%. Regarding the patients’ analysis, 98% of the compounds in plasma were detected in Mitra<sup>TM</sup> compared to whole blood, with a satisfying concordance (R<sup>2</sup> = 0.827). Our novel screening approach opens new insights into different toxicologic fields appropriate for pediatrics, forensics or to perform mass screening. |
| first_indexed | 2024-03-11T04:41:56Z |
| format | Article |
| id | doaj.art-cfc72a9dd3004f07892b0b0e19e5ae1d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:41:56Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cfc72a9dd3004f07892b0b0e19e5ae1d2023-11-17T20:39:21ZengMDPI AGMolecules1420-30492023-04-01288346610.3390/molecules28083466New Trend in Toxicological Screening Using Volumetric Absorptive Microsampling (VAMS) and High-Resolution Mass Spectrometry (HR/MS) CombinationPascal Houzé0Ilona Borowski1Eugénie Bito2Romain Magny3Athina Morcos4Sebastian Voicu5Bruno Mégarbane6Laurence Labat7Laboratory of Toxicology, Federation of Toxicology, Lariboisière Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), 10 rue Ambroise Paré, 75010 Paris, FranceLaboratory of Toxicology, Federation of Toxicology, Lariboisière Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), 10 rue Ambroise Paré, 75010 Paris, FranceLaboratory of Toxicology, Federation of Toxicology, Lariboisière Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), 10 rue Ambroise Paré, 75010 Paris, FranceLaboratory of Toxicology, Federation of Toxicology, Lariboisière Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), 10 rue Ambroise Paré, 75010 Paris, FranceLaboratory of Toxicology, Federation of Toxicology, Lariboisière Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), 10 rue Ambroise Paré, 75010 Paris, FranceINSERM UMRS-1144, University of Paris, 4 Avenue de l’Observatoire, 75006 Paris, FranceINSERM UMRS-1144, University of Paris, 4 Avenue de l’Observatoire, 75006 Paris, FranceLaboratory of Toxicology, Federation of Toxicology, Lariboisière Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), 10 rue Ambroise Paré, 75010 Paris, FranceIn toxicology, screenings are routinely performed using chromatographic methods coupled to detection systems such as high-resolution mass spectrometry (HR/MS). The increase in specificity and sensitivity of HRMS is responsible for the development of methods for alternative samples such as Volumetric Adsorptive Micro-Sampling. Whole blood overloaded with 90 drugs was sampled with 20 µL Mitra<sup>TM</sup> to optimize the pre-analytical step as well as to determine the identification limits of drugs. Elution of chemicals was carried out in a solvent mixture through agitation and sonication. After dissolution, 10 μL was injected into the chromatographic system coupled to the Orbitrap<sup>TM</sup> HR/MS. Compounds were confirmed against the laboratory library. The clinical feasibility was assessed in fifteen poisoned patients using the simultaneous sampling of plasma, whole blood and Mitra<sup>TM</sup>. The optimized extraction procedure allowed us to confirm 87 compounds out of the 90 present in the spiked whole blood. Cannabis derivatives were not detected. For 82.2% of the investigated drugs, the identification limits were below 12.5 ng·mL<sup>−1</sup>, with the extraction yields ranging from 80.6 to 108.7%. Regarding the patients’ analysis, 98% of the compounds in plasma were detected in Mitra<sup>TM</sup> compared to whole blood, with a satisfying concordance (R<sup>2</sup> = 0.827). Our novel screening approach opens new insights into different toxicologic fields appropriate for pediatrics, forensics or to perform mass screening.https://www.mdpi.com/1420-3049/28/8/3466toxicological screeningwhole bloodvolumetric absorptive microsampling (VAMS)Mitra<sup>TM</sup> devicehigh-resolution mass spectrometryclinical application |
| spellingShingle | Pascal Houzé Ilona Borowski Eugénie Bito Romain Magny Athina Morcos Sebastian Voicu Bruno Mégarbane Laurence Labat New Trend in Toxicological Screening Using Volumetric Absorptive Microsampling (VAMS) and High-Resolution Mass Spectrometry (HR/MS) Combination Molecules toxicological screening whole blood volumetric absorptive microsampling (VAMS) Mitra<sup>TM</sup> device high-resolution mass spectrometry clinical application |
| title | New Trend in Toxicological Screening Using Volumetric Absorptive Microsampling (VAMS) and High-Resolution Mass Spectrometry (HR/MS) Combination |
| title_full | New Trend in Toxicological Screening Using Volumetric Absorptive Microsampling (VAMS) and High-Resolution Mass Spectrometry (HR/MS) Combination |
| title_fullStr | New Trend in Toxicological Screening Using Volumetric Absorptive Microsampling (VAMS) and High-Resolution Mass Spectrometry (HR/MS) Combination |
| title_full_unstemmed | New Trend in Toxicological Screening Using Volumetric Absorptive Microsampling (VAMS) and High-Resolution Mass Spectrometry (HR/MS) Combination |
| title_short | New Trend in Toxicological Screening Using Volumetric Absorptive Microsampling (VAMS) and High-Resolution Mass Spectrometry (HR/MS) Combination |
| title_sort | new trend in toxicological screening using volumetric absorptive microsampling vams and high resolution mass spectrometry hr ms combination |
| topic | toxicological screening whole blood volumetric absorptive microsampling (VAMS) Mitra<sup>TM</sup> device high-resolution mass spectrometry clinical application |
| url | https://www.mdpi.com/1420-3049/28/8/3466 |
| work_keys_str_mv | AT pascalhouze newtrendintoxicologicalscreeningusingvolumetricabsorptivemicrosamplingvamsandhighresolutionmassspectrometryhrmscombination AT ilonaborowski newtrendintoxicologicalscreeningusingvolumetricabsorptivemicrosamplingvamsandhighresolutionmassspectrometryhrmscombination AT eugeniebito newtrendintoxicologicalscreeningusingvolumetricabsorptivemicrosamplingvamsandhighresolutionmassspectrometryhrmscombination AT romainmagny newtrendintoxicologicalscreeningusingvolumetricabsorptivemicrosamplingvamsandhighresolutionmassspectrometryhrmscombination AT athinamorcos newtrendintoxicologicalscreeningusingvolumetricabsorptivemicrosamplingvamsandhighresolutionmassspectrometryhrmscombination AT sebastianvoicu newtrendintoxicologicalscreeningusingvolumetricabsorptivemicrosamplingvamsandhighresolutionmassspectrometryhrmscombination AT brunomegarbane newtrendintoxicologicalscreeningusingvolumetricabsorptivemicrosamplingvamsandhighresolutionmassspectrometryhrmscombination AT laurencelabat newtrendintoxicologicalscreeningusingvolumetricabsorptivemicrosamplingvamsandhighresolutionmassspectrometryhrmscombination |