Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia.
BACKGROUND:Enterotoxigenic Bacteroides fragilis (ETBF) is a toxin-producing bacteria thought to possibly promote colorectal carcinogenesis by modulating the mucosal immune response and inducing epithelial cell changes. Here, we aim to examine the association of colonic mucosal colonization with ETBF...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5289627?pdf=render |
| _version_ | 1818037569232830464 |
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| author | Rachel V Purcell John Pearson Alan Aitchison Liane Dixon Frank A Frizelle Jacqueline I Keenan |
| author_facet | Rachel V Purcell John Pearson Alan Aitchison Liane Dixon Frank A Frizelle Jacqueline I Keenan |
| author_sort | Rachel V Purcell |
| collection | DOAJ |
| description | BACKGROUND:Enterotoxigenic Bacteroides fragilis (ETBF) is a toxin-producing bacteria thought to possibly promote colorectal carcinogenesis by modulating the mucosal immune response and inducing epithelial cell changes. Here, we aim to examine the association of colonic mucosal colonization with ETBF and the presence of a range of lesions on the colonic neoplastic spectrum. METHODS:Mucosal tissue from up to four different colonic sites was obtained from a consecutive series of 150 patients referred for colonoscopy. The presence and relative abundance of the B. fragilis toxin gene (bft) in each tissue sample was determined using quantitative PCR, and associations with clinicopathological characteristics were analysed. FINDINGS:We found a high concordance of ETBF between different colonic sites (86%). Univariate analysis showed statistically significant associations between ETBF positivity and the presence of low-grade dysplasia (LGD), tubular adenomas (TA), and serrated polyps (P-values of 0.007, 0.027, and 0.007, respectively). A higher relative abundance of ETBF was significantly associated with LGD and TA (P-values of < 0.0001 and 0.025, respectively). Increased ETBF positivity and abundance was also associated with left-sided biopsies, compared to those from the right side of the colon. CONCLUSION:Our results showing association of ETBF positivity and increased abundance with early-stage carcinogenic lesions underlines its importance in the development of colorectal cancer, and we suggest that detection of ETBF may be a potential marker of early colorectal carcinogenesis. |
| first_indexed | 2024-12-10T07:28:56Z |
| format | Article |
| id | doaj.art-cfc7ae0a87984cd08fb3e2c85159717c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:28:56Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cfc7ae0a87984cd08fb3e2c85159717c2022-12-22T01:57:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017160210.1371/journal.pone.0171602Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia.Rachel V PurcellJohn PearsonAlan AitchisonLiane DixonFrank A FrizelleJacqueline I KeenanBACKGROUND:Enterotoxigenic Bacteroides fragilis (ETBF) is a toxin-producing bacteria thought to possibly promote colorectal carcinogenesis by modulating the mucosal immune response and inducing epithelial cell changes. Here, we aim to examine the association of colonic mucosal colonization with ETBF and the presence of a range of lesions on the colonic neoplastic spectrum. METHODS:Mucosal tissue from up to four different colonic sites was obtained from a consecutive series of 150 patients referred for colonoscopy. The presence and relative abundance of the B. fragilis toxin gene (bft) in each tissue sample was determined using quantitative PCR, and associations with clinicopathological characteristics were analysed. FINDINGS:We found a high concordance of ETBF between different colonic sites (86%). Univariate analysis showed statistically significant associations between ETBF positivity and the presence of low-grade dysplasia (LGD), tubular adenomas (TA), and serrated polyps (P-values of 0.007, 0.027, and 0.007, respectively). A higher relative abundance of ETBF was significantly associated with LGD and TA (P-values of < 0.0001 and 0.025, respectively). Increased ETBF positivity and abundance was also associated with left-sided biopsies, compared to those from the right side of the colon. CONCLUSION:Our results showing association of ETBF positivity and increased abundance with early-stage carcinogenic lesions underlines its importance in the development of colorectal cancer, and we suggest that detection of ETBF may be a potential marker of early colorectal carcinogenesis.http://europepmc.org/articles/PMC5289627?pdf=render |
| spellingShingle | Rachel V Purcell John Pearson Alan Aitchison Liane Dixon Frank A Frizelle Jacqueline I Keenan Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia. PLoS ONE |
| title | Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia. |
| title_full | Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia. |
| title_fullStr | Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia. |
| title_full_unstemmed | Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia. |
| title_short | Colonization with enterotoxigenic Bacteroides fragilis is associated with early-stage colorectal neoplasia. |
| title_sort | colonization with enterotoxigenic bacteroides fragilis is associated with early stage colorectal neoplasia |
| url | http://europepmc.org/articles/PMC5289627?pdf=render |
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