Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates
Nonhuman primates (NHPs) are relevant models to study the pathogenesis of tuberculosis (TB) and evaluate the potential of TB therapies, but rapid tools allowing diagnosis of active pulmonary TB in NHPs are lacking. This study investigates whether low complexity lateral flow assays utilizing upconver...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/10/12/1260 |
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| author | Zijie Zhou Anouk van Hooij Richard Vervenne Claudia C. Sombroek Elisa M. Tjon Kon Fat Tom H. M. Ottenhoff Paul L. A. M. Corstjens Frank Verreck Annemieke Geluk |
| author_facet | Zijie Zhou Anouk van Hooij Richard Vervenne Claudia C. Sombroek Elisa M. Tjon Kon Fat Tom H. M. Ottenhoff Paul L. A. M. Corstjens Frank Verreck Annemieke Geluk |
| author_sort | Zijie Zhou |
| collection | DOAJ |
| description | Nonhuman primates (NHPs) are relevant models to study the pathogenesis of tuberculosis (TB) and evaluate the potential of TB therapies, but rapid tools allowing diagnosis of active pulmonary TB in NHPs are lacking. This study investigates whether low complexity lateral flow assays utilizing upconverting reporter particles (UCP-LFAs) developed for rapid detection of human serum proteins can be applied to detect and monitor active pulmonary TB in NHPs. UCP-LFAs were used to assess serum proteins levels and changes in relation to the MTB challenge dosage, lung pathology, treatment, and disease outcome in experimentally MTB-infected macaques. Serum levels of SAA1, IP-10, and IL-6 showed a significant increase after MTB infection in rhesus macaques and correlated with disease severity as determined by pathology scoring. Moreover, these biomarkers could sensitively detect the reduction of bacterial levels in the lungs of macaques due to BCG vaccination or drug treatment. Quantitative measurements by rapid UCP-LFAs specific for SAA1, IP-10, and IL-6 in serum can be utilized to detect active progressive pulmonary TB in macaques. The UCP-LFAs thus offer a low-cost, convenient, and minimally invasive diagnostic tool that can be applied in studies on TB vaccine and drug development involving macaques. |
| first_indexed | 2024-03-10T04:35:07Z |
| format | Article |
| id | doaj.art-cfcbe3c23ac7465393b01836dfc19347 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T04:35:07Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-cfcbe3c23ac7465393b01836dfc193472023-11-23T03:53:19ZengMDPI AGBiology2079-77372021-12-011012126010.3390/biology10121260Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman PrimatesZijie Zhou0Anouk van Hooij1Richard Vervenne2Claudia C. Sombroek3Elisa M. Tjon Kon Fat4Tom H. M. Ottenhoff5Paul L. A. M. Corstjens6Frank Verreck7Annemieke Geluk8Department of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The NetherlandsDepartment of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The NetherlandsSection of TB Research & Immunology, Biomedical Primate Research Center (BPRC), 2288 GJ Rijswijk, The NetherlandsSection of TB Research & Immunology, Biomedical Primate Research Center (BPRC), 2288 GJ Rijswijk, The NetherlandsDepartment of Cell and Chemical Biology, Leiden University Medical Center, 2333 ZA Leiden, The NetherlandsDepartment of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The NetherlandsDepartment of Cell and Chemical Biology, Leiden University Medical Center, 2333 ZA Leiden, The NetherlandsSection of TB Research & Immunology, Biomedical Primate Research Center (BPRC), 2288 GJ Rijswijk, The NetherlandsDepartment of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The NetherlandsNonhuman primates (NHPs) are relevant models to study the pathogenesis of tuberculosis (TB) and evaluate the potential of TB therapies, but rapid tools allowing diagnosis of active pulmonary TB in NHPs are lacking. This study investigates whether low complexity lateral flow assays utilizing upconverting reporter particles (UCP-LFAs) developed for rapid detection of human serum proteins can be applied to detect and monitor active pulmonary TB in NHPs. UCP-LFAs were used to assess serum proteins levels and changes in relation to the MTB challenge dosage, lung pathology, treatment, and disease outcome in experimentally MTB-infected macaques. Serum levels of SAA1, IP-10, and IL-6 showed a significant increase after MTB infection in rhesus macaques and correlated with disease severity as determined by pathology scoring. Moreover, these biomarkers could sensitively detect the reduction of bacterial levels in the lungs of macaques due to BCG vaccination or drug treatment. Quantitative measurements by rapid UCP-LFAs specific for SAA1, IP-10, and IL-6 in serum can be utilized to detect active progressive pulmonary TB in macaques. The UCP-LFAs thus offer a low-cost, convenient, and minimally invasive diagnostic tool that can be applied in studies on TB vaccine and drug development involving macaques.https://www.mdpi.com/2079-7737/10/12/1260biomarkersdiagnosisnonhuman primates (NHPs)lateral flow assayup-converting reporter particlesMTB |
| spellingShingle | Zijie Zhou Anouk van Hooij Richard Vervenne Claudia C. Sombroek Elisa M. Tjon Kon Fat Tom H. M. Ottenhoff Paul L. A. M. Corstjens Frank Verreck Annemieke Geluk Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates Biology biomarkers diagnosis nonhuman primates (NHPs) lateral flow assay up-converting reporter particles MTB |
| title | Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates |
| title_full | Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates |
| title_fullStr | Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates |
| title_full_unstemmed | Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates |
| title_short | Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates |
| title_sort | quantitative rapid test for detection and monitoring of active pulmonary tuberculosis in nonhuman primates |
| topic | biomarkers diagnosis nonhuman primates (NHPs) lateral flow assay up-converting reporter particles MTB |
| url | https://www.mdpi.com/2079-7737/10/12/1260 |
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