mRNA-seq whole transcriptome profiling of fresh frozen versus archived fixed tissues

mRNA-seq whole transcriptome profiling of fresh frozen versus archived fixed tissues

Abstract Background The main bottleneck for genomic studies of tumors is the limited availability of fresh frozen (FF) samples collected from patients, coupled with comprehensive long-term clinical follow-up. This shortage could be alleviated by using existing large archives of routinely obtained an...

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Bibliographic Details
Main Authors: Noa Bossel Ben-Moshe, Shlomit Gilad, Gili Perry, Sima Benjamin, Nora Balint-Lahat, Anya Pavlovsky, Sharon Halperin, Barak Markus, Ady Yosepovich, Iris Barshack, Einav Nili Gal-Yam, Eytan Domany, Bella Kaufman, Maya Dadiani
Format: Article
Language:English
Published: BMC 2018-05-01
Series:BMC Genomics
Subjects:
RNA sequencing
FFPE
Poly-a capturing
Ribosomal depletion
Breast cancer
Online Access:http://link.springer.com/article/10.1186/s12864-018-4761-3

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