Geobacter sulfurreducens metabolism at different donor/acceptor ratios
Abstract Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target‐oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-10-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.1322 |
| _version_ | 1798045537878933504 |
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| author | Hanna Marianne Frühauf‐Wyllie Dirk Holtmann |
| author_facet | Hanna Marianne Frühauf‐Wyllie Dirk Holtmann |
| author_sort | Hanna Marianne Frühauf‐Wyllie |
| collection | DOAJ |
| description | Abstract Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target‐oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate, while fumarate is the usual electron acceptor. Here, we could show that depending on the donor/acceptor ratio in batch cultivation of Geobacter sulfurreducens different product patterns occur. With a donor/acceptor ratio of 1:2.5 malate accumulated as an intermediate product but was metabolized to succinate subsequently. At the end of the cultivation, the ratio of fumarate consumed and succinate produced was approximately 1:1. When fumarate was added in excess, malate accumulated in the fermentation broth without further metabolization. After the addition of acetate to stationary cells, malate concentration decreased immediately and additional succinate was synthesized. Finally, it was shown that also resting cells of G. sulfurreducens could efficiently convert fumarate to malate without an additional electron donor. Overall, it was demonstrated that by altering the donor/acceptor ratio, targeted optimization of the metabolite conversion by G. sulfurreducens can be realized. |
| first_indexed | 2024-04-11T23:22:35Z |
| format | Article |
| id | doaj.art-cfce5f5bbb854ac089305496501d11fc |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-04-11T23:22:35Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-cfce5f5bbb854ac089305496501d11fc2022-12-22T03:57:25ZengWileyMicrobiologyOpen2045-88272022-10-01115n/an/a10.1002/mbo3.1322Geobacter sulfurreducens metabolism at different donor/acceptor ratiosHanna Marianne Frühauf‐Wyllie0Dirk Holtmann1Department of Chemical Technology DECHEMA Forschungsinstitut Frankfurt am Main GermanyTechnische Hochschule Mittelhessen, Institut für Bioverfahrenstechnik und Pharmazeutische Technologie Gießen GermanyAbstract Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target‐oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate, while fumarate is the usual electron acceptor. Here, we could show that depending on the donor/acceptor ratio in batch cultivation of Geobacter sulfurreducens different product patterns occur. With a donor/acceptor ratio of 1:2.5 malate accumulated as an intermediate product but was metabolized to succinate subsequently. At the end of the cultivation, the ratio of fumarate consumed and succinate produced was approximately 1:1. When fumarate was added in excess, malate accumulated in the fermentation broth without further metabolization. After the addition of acetate to stationary cells, malate concentration decreased immediately and additional succinate was synthesized. Finally, it was shown that also resting cells of G. sulfurreducens could efficiently convert fumarate to malate without an additional electron donor. Overall, it was demonstrated that by altering the donor/acceptor ratio, targeted optimization of the metabolite conversion by G. sulfurreducens can be realized.https://doi.org/10.1002/mbo3.1322central metabolismdonor/acceptor ratioGeobacter sulfurreducensorganic acid production |
| spellingShingle | Hanna Marianne Frühauf‐Wyllie Dirk Holtmann Geobacter sulfurreducens metabolism at different donor/acceptor ratios MicrobiologyOpen central metabolism donor/acceptor ratio Geobacter sulfurreducens organic acid production |
| title | Geobacter sulfurreducens metabolism at different donor/acceptor ratios |
| title_full | Geobacter sulfurreducens metabolism at different donor/acceptor ratios |
| title_fullStr | Geobacter sulfurreducens metabolism at different donor/acceptor ratios |
| title_full_unstemmed | Geobacter sulfurreducens metabolism at different donor/acceptor ratios |
| title_short | Geobacter sulfurreducens metabolism at different donor/acceptor ratios |
| title_sort | geobacter sulfurreducens metabolism at different donor acceptor ratios |
| topic | central metabolism donor/acceptor ratio Geobacter sulfurreducens organic acid production |
| url | https://doi.org/10.1002/mbo3.1322 |
| work_keys_str_mv | AT hannamariannefruhaufwyllie geobactersulfurreducensmetabolismatdifferentdonoracceptorratios AT dirkholtmann geobactersulfurreducensmetabolismatdifferentdonoracceptorratios |