Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR
The basic requirement for probiotic bacteria to be able to perform expected positive effects is to be alive. Therefore, appropriate quantification methods are crucial. Bacterial quantification based on nucleic acid detection is increasingly used. Spray-drying (SD) is one of the possibilities to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2012-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641204465R.pdf |
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| author | Radulović Zorica Mirković N. Bogović-Matijašič Bojana Petrušić Milica Petrović Tanja Manojlović Verica Nedović V. |
| author_facet | Radulović Zorica Mirković N. Bogović-Matijašič Bojana Petrušić Milica Petrović Tanja Manojlović Verica Nedović V. |
| author_sort | Radulović Zorica |
| collection | DOAJ |
| description | The basic requirement for probiotic bacteria to be able to perform expected positive effects is to be alive. Therefore, appropriate quantification methods are crucial. Bacterial quantification based on nucleic acid detection is increasingly used. Spray-drying (SD) is one of the possibilities to improve the survival of probiotic bacteria against negative environmental effects. The aim of this study was to investigate the survival of spray-dried Lactobacillus plantarum 564 and Lactobacillus paracasei Z-8, and to investigate the impact on some probiotic properties caused by SD of both tested strains. Besides the plate count technique, the aim was to examine the possibility of using propidium monoazide (PMA) in combination with real-time polymerase chain reaction (PCR) for determining spray-dried tested strains. The number of intact cells, Lb. plantarum 564 and Lb. paracasei Z-8, was determined by real-time PCR with PMA, and it was similar to the number of investigated strains obtained by the plate count method. Spray-dried Lb. plantarum 564 and Lb. paracasei Z-8 demonstrated very good probiotic ability. It may be concluded that the PMA real-time PCR determination of the viability of probiotic bacteria could complement the plate count method and SD may be a cost-effective way to produce large quantities of some probiotic cultures. [Projekat Ministarstva nauke Republike Srbije, br. 046010] |
| first_indexed | 2024-12-12T23:00:54Z |
| format | Article |
| id | doaj.art-cfce767ea1354f5da08b2a9799a4cca7 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-12T23:00:54Z |
| publishDate | 2012-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-cfce767ea1354f5da08b2a9799a4cca72022-12-22T00:08:49ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392012-01-016441465147210.2298/ABS1204465RQuantification of viable spray-dried potential probiotic lactobacilli using real-time PCRRadulović ZoricaMirković N.Bogović-Matijašič BojanaPetrušić MilicaPetrović TanjaManojlović VericaNedović V.The basic requirement for probiotic bacteria to be able to perform expected positive effects is to be alive. Therefore, appropriate quantification methods are crucial. Bacterial quantification based on nucleic acid detection is increasingly used. Spray-drying (SD) is one of the possibilities to improve the survival of probiotic bacteria against negative environmental effects. The aim of this study was to investigate the survival of spray-dried Lactobacillus plantarum 564 and Lactobacillus paracasei Z-8, and to investigate the impact on some probiotic properties caused by SD of both tested strains. Besides the plate count technique, the aim was to examine the possibility of using propidium monoazide (PMA) in combination with real-time polymerase chain reaction (PCR) for determining spray-dried tested strains. The number of intact cells, Lb. plantarum 564 and Lb. paracasei Z-8, was determined by real-time PCR with PMA, and it was similar to the number of investigated strains obtained by the plate count method. Spray-dried Lb. plantarum 564 and Lb. paracasei Z-8 demonstrated very good probiotic ability. It may be concluded that the PMA real-time PCR determination of the viability of probiotic bacteria could complement the plate count method and SD may be a cost-effective way to produce large quantities of some probiotic cultures. [Projekat Ministarstva nauke Republike Srbije, br. 046010]http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641204465R.pdfSpray-dryingpotential probioticreal-time PCRpropidium monoazide |
| spellingShingle | Radulović Zorica Mirković N. Bogović-Matijašič Bojana Petrušić Milica Petrović Tanja Manojlović Verica Nedović V. Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR Archives of Biological Sciences Spray-drying potential probiotic real-time PCR propidium monoazide |
| title | Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR |
| title_full | Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR |
| title_fullStr | Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR |
| title_full_unstemmed | Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR |
| title_short | Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR |
| title_sort | quantification of viable spray dried potential probiotic lactobacilli using real time pcr |
| topic | Spray-drying potential probiotic real-time PCR propidium monoazide |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641204465R.pdf |
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