Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA
Abstract Unmethylated CpG oligodeoxynucleotides are potent immunostimulatory motifs in activating both innate and acquired immune system by inducing Th1 type antigen-specific T cell responses, but their instability in serum greatly influences their immunostimulant efficiency. Here, we constructed a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2019-06-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11671-019-3032-9 |
| _version_ | 1797711467274829824 |
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| author | Jiana Jiang Xianming Kong Yuexia Xie Hanbing Zou Qianyun Tang Ding Ma Xue Zhao Xiaozhen He Anyue Xia Peifeng Liu |
| author_facet | Jiana Jiang Xianming Kong Yuexia Xie Hanbing Zou Qianyun Tang Ding Ma Xue Zhao Xiaozhen He Anyue Xia Peifeng Liu |
| author_sort | Jiana Jiang |
| collection | DOAJ |
| description | Abstract Unmethylated CpG oligodeoxynucleotides are potent immunostimulatory motifs in activating both innate and acquired immune system by inducing Th1 type antigen-specific T cell responses, but their instability in serum greatly influences their immunostimulant efficiency. Here, we constructed a novel immuno-DNA nanohydrogels consisting of tandem repeat sequences of CpG units named CpG-MCA nanohydrogels through multi-primed chain amplification. CpG-MCA nanohydrogels were proved to resist degradation and increase the proliferation and migration of murine macrophage-like RAW264.7 cells. Furthermore, CpG-MCA nanohydrogels effectively induced high expression of tumor necrosis factor-α and interleukin-6, and remarkably inhibited the proliferation of U251 cells, suggesting that CpG-MCA nanohydrogels are expected to be employed as the potent anti-cancer immunostimulant. |
| first_indexed | 2024-03-12T07:07:32Z |
| format | Article |
| id | doaj.art-cfd0b942c4804bbd948c7f668cb6922e |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T07:07:32Z |
| publishDate | 2019-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-cfd0b942c4804bbd948c7f668cb6922e2023-09-02T23:20:52ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2019-06-0114111010.1186/s11671-019-3032-9Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNAJiana Jiang0Xianming Kong1Yuexia Xie2Hanbing Zou3Qianyun Tang4Ding Ma5Xue Zhao6Xiaozhen He7Anyue Xia8Peifeng Liu9Central Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityCentral Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityCentral Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityCentral Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityState Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityCentral Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityCentral Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityCentral Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityCentral Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityCentral Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityAbstract Unmethylated CpG oligodeoxynucleotides are potent immunostimulatory motifs in activating both innate and acquired immune system by inducing Th1 type antigen-specific T cell responses, but their instability in serum greatly influences their immunostimulant efficiency. Here, we constructed a novel immuno-DNA nanohydrogels consisting of tandem repeat sequences of CpG units named CpG-MCA nanohydrogels through multi-primed chain amplification. CpG-MCA nanohydrogels were proved to resist degradation and increase the proliferation and migration of murine macrophage-like RAW264.7 cells. Furthermore, CpG-MCA nanohydrogels effectively induced high expression of tumor necrosis factor-α and interleukin-6, and remarkably inhibited the proliferation of U251 cells, suggesting that CpG-MCA nanohydrogels are expected to be employed as the potent anti-cancer immunostimulant.http://link.springer.com/article/10.1186/s11671-019-3032-9CpGNanohydrogelImmunotherapyMacrophageGlioma |
| spellingShingle | Jiana Jiang Xianming Kong Yuexia Xie Hanbing Zou Qianyun Tang Ding Ma Xue Zhao Xiaozhen He Anyue Xia Peifeng Liu Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA Nanoscale Research Letters CpG Nanohydrogel Immunotherapy Macrophage Glioma |
| title | Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA |
| title_full | Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA |
| title_fullStr | Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA |
| title_full_unstemmed | Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA |
| title_short | Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA |
| title_sort | potent anti tumor immunostimulatory biocompatible nanohydrogel made from dna |
| topic | CpG Nanohydrogel Immunotherapy Macrophage Glioma |
| url | http://link.springer.com/article/10.1186/s11671-019-3032-9 |
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