HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCs
Abstract The complete regeneration of deer antlers is based on the proliferation and differentiation of stem cells. Mesenchymal stem cells (MSCs) of antlers have an important role in antler regeneration and rapid growth and development. HGF is mainly synthesized and secreted by mesenchymal cells. Af...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38116-7 |
| _version_ | 1797778930306908160 |
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| author | Miao Wang Chuan Lin Xiaodong Jia Di Fang Qinhua Gao Chunmei Han |
| author_facet | Miao Wang Chuan Lin Xiaodong Jia Di Fang Qinhua Gao Chunmei Han |
| author_sort | Miao Wang |
| collection | DOAJ |
| description | Abstract The complete regeneration of deer antlers is based on the proliferation and differentiation of stem cells. Mesenchymal stem cells (MSCs) of antlers have an important role in antler regeneration and rapid growth and development. HGF is mainly synthesized and secreted by mesenchymal cells. After binding to its receptor c-Met, which transduces signals into cells to stimulate cell proliferation and migration in various organs to promote tissue morphogenesis and angiogenesis. However, the role and mechanism of the HGF/c-Met signaling pathway on antler MSCs are still unclear. In this study, we established antler MSCs with overexpression and interference of HGF gene by lentivirus and small interference RNA, observed the effect of HGF/c-Met signal pathway on the proliferation and migration of antler MSCs, and detected the expression of downstream related signal pathway genes, to explore the mechanism of HGF/c-MET signal pathway on the proliferation and migration of antler MSCs. The results showed that the HGF/c-Met signal affects the expression of RAS, ERK and MEK genes, regulates the proliferation of pilose antler MSCs through Ras/Raf, MEK/ERK pathway, affects the expression of Gab1, Grb2, AKT and PI3K genes, and regulates the migration of MSCs of pilose antler through Gab1/Grb2 and PI3K/AKT pathway. |
| first_indexed | 2024-03-12T23:23:42Z |
| format | Article |
| id | doaj.art-cfd2cb420f944a739922652a1429bd25 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T23:23:42Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-cfd2cb420f944a739922652a1429bd252023-07-16T11:17:04ZengNature PortfolioScientific Reports2045-23222023-07-011311910.1038/s41598-023-38116-7HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCsMiao Wang0Chuan Lin1Xiaodong Jia2Di Fang3Qinhua Gao4Chunmei Han5College of Animal Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityCollege of Life Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityAbstract The complete regeneration of deer antlers is based on the proliferation and differentiation of stem cells. Mesenchymal stem cells (MSCs) of antlers have an important role in antler regeneration and rapid growth and development. HGF is mainly synthesized and secreted by mesenchymal cells. After binding to its receptor c-Met, which transduces signals into cells to stimulate cell proliferation and migration in various organs to promote tissue morphogenesis and angiogenesis. However, the role and mechanism of the HGF/c-Met signaling pathway on antler MSCs are still unclear. In this study, we established antler MSCs with overexpression and interference of HGF gene by lentivirus and small interference RNA, observed the effect of HGF/c-Met signal pathway on the proliferation and migration of antler MSCs, and detected the expression of downstream related signal pathway genes, to explore the mechanism of HGF/c-MET signal pathway on the proliferation and migration of antler MSCs. The results showed that the HGF/c-Met signal affects the expression of RAS, ERK and MEK genes, regulates the proliferation of pilose antler MSCs through Ras/Raf, MEK/ERK pathway, affects the expression of Gab1, Grb2, AKT and PI3K genes, and regulates the migration of MSCs of pilose antler through Gab1/Grb2 and PI3K/AKT pathway.https://doi.org/10.1038/s41598-023-38116-7 |
| spellingShingle | Miao Wang Chuan Lin Xiaodong Jia Di Fang Qinhua Gao Chunmei Han HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCs Scientific Reports |
| title | HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCs |
| title_full | HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCs |
| title_fullStr | HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCs |
| title_full_unstemmed | HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCs |
| title_short | HGF/c-Met signaling promotes the migration and proliferation of deer antler MSCs |
| title_sort | hgf c met signaling promotes the migration and proliferation of deer antler mscs |
| url | https://doi.org/10.1038/s41598-023-38116-7 |
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