Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant species
The discovery of new antibiotics to overcome the growing resistance problem as well as the discovery of new natural, safe antioxidants to combat oxidative stress are still urgently needed. Medicinal plants are known to produce potential therapeutic substances which are more biologically selective th...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2021-09-01
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| Series: | Indonesian Journal of Pharmacy |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/v3/IJP/article/view/1774 |
| _version_ | 1819005646608007168 |
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| author | Marlin Megalestin Raunsai Kartika Dyah Palupi Ahmad Fathoni Andria Agusta |
| author_facet | Marlin Megalestin Raunsai Kartika Dyah Palupi Ahmad Fathoni Andria Agusta |
| author_sort | Marlin Megalestin Raunsai |
| collection | DOAJ |
| description | The discovery of new antibiotics to overcome the growing resistance problem as well as the discovery of new natural, safe antioxidants to combat oxidative stress are still urgently needed. Medicinal plants are known to produce potential therapeutic substances which are more biologically selective than synthetic compounds. Therefore, we explored the bioactivities of 35 ethanolic extracts from 24 underexplored plant species collected in Halmahera, to find potential sources for antibacterial and antioxidant agents.
Dried plant parts were extracted using ethanol 96%. Thin layer chromatography-direct-bioautography (TLC-DB) and minimum inhibitory concentration (MIC) determination were used to evaluate the antibacterial effect. Antioxidant activity was determined against DPPH using TLC-DB and microdilution assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu’s method.
The ethanolic extracts exhibited moderate to weak antibacterial activity against Escherichia coli and Staphylococcus aureus. However, the leaf extract of Elaeocarpus dolichostylus, Elaeocarpus multiflorus, and Psychotria celebica as well as the stem bark extract of Elaeocarpus dolichostylus, Cinnamomum sintoc, and Garcinia latissima displayed very strong antioxidant activities against DPPH with AAI values between 4.60 to 13.42. A strong correlation between TPC and antioxidant activity with r = 0.8712 was observed.
Despite the moderate to weak antibacterial activity, eight underexplored plant species exhibit strong antioxidant activities. A high correlation between TPC and antioxidant activity indicating a prominent role of phenolic compounds in the plants’ antioxidant properties. These findings indicate that collected plants from Halmahera are potential to be studied and developed further as the potential sources for novel antioxidants. |
| first_indexed | 2024-12-20T23:56:06Z |
| format | Article |
| id | doaj.art-cfd4aa8a4ccf414880e40461d365732e |
| institution | Directory Open Access Journal |
| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-12-20T23:56:06Z |
| publishDate | 2021-09-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-cfd4aa8a4ccf414880e40461d365732e2022-12-21T19:22:44ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862021-09-0137638410.22146/ijp.17741774Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant speciesMarlin Megalestin Raunsai0Kartika Dyah Palupi1Ahmad Fathoni2Andria Agusta3Research Center for Chemistry, Indonesian Institute of Sciences, Jalan puspitek serpong Gate, Muncul, Kec. Serpong, Kota Tangerang Selatan, Banten 15314Research Center for Chemistry, Indonesian Institute of Sciences, Jalan puspitek serpong Gate, Muncul, Kec. Serpong, Kota Tangerang Selatan, Banten 15314Research Center for Chemistry, Indonesian Institute of Sciences, Jalan puspitek serpong Gate, Muncul, Kec. Serpong, Kota Tangerang Selatan, Banten 15314Research Center for Chemistry, Indonesian Institute of Sciences, Jalan puspitek serpong Gate, Muncul, Kec. Serpong, Kota Tangerang Selatan, Banten 15314The discovery of new antibiotics to overcome the growing resistance problem as well as the discovery of new natural, safe antioxidants to combat oxidative stress are still urgently needed. Medicinal plants are known to produce potential therapeutic substances which are more biologically selective than synthetic compounds. Therefore, we explored the bioactivities of 35 ethanolic extracts from 24 underexplored plant species collected in Halmahera, to find potential sources for antibacterial and antioxidant agents. Dried plant parts were extracted using ethanol 96%. Thin layer chromatography-direct-bioautography (TLC-DB) and minimum inhibitory concentration (MIC) determination were used to evaluate the antibacterial effect. Antioxidant activity was determined against DPPH using TLC-DB and microdilution assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu’s method. The ethanolic extracts exhibited moderate to weak antibacterial activity against Escherichia coli and Staphylococcus aureus. However, the leaf extract of Elaeocarpus dolichostylus, Elaeocarpus multiflorus, and Psychotria celebica as well as the stem bark extract of Elaeocarpus dolichostylus, Cinnamomum sintoc, and Garcinia latissima displayed very strong antioxidant activities against DPPH with AAI values between 4.60 to 13.42. A strong correlation between TPC and antioxidant activity with r = 0.8712 was observed. Despite the moderate to weak antibacterial activity, eight underexplored plant species exhibit strong antioxidant activities. A high correlation between TPC and antioxidant activity indicating a prominent role of phenolic compounds in the plants’ antioxidant properties. These findings indicate that collected plants from Halmahera are potential to be studied and developed further as the potential sources for novel antioxidants.https://jurnal.ugm.ac.id/v3/IJP/article/view/1774antibacterial, antioxidant, medicinal plants, phenolic content, halmahera island |
| spellingShingle | Marlin Megalestin Raunsai Kartika Dyah Palupi Ahmad Fathoni Andria Agusta Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant species Indonesian Journal of Pharmacy antibacterial, antioxidant, medicinal plants, phenolic content, halmahera island |
| title | Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant species |
| title_full | Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant species |
| title_fullStr | Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant species |
| title_full_unstemmed | Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant species |
| title_short | Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant species |
| title_sort | bioactivities of plant extracts collected in halmahera indonesia a bioprospection study of underexplored plant species |
| topic | antibacterial, antioxidant, medicinal plants, phenolic content, halmahera island |
| url | https://jurnal.ugm.ac.id/v3/IJP/article/view/1774 |
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