Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-Metabolomics
Insufficient calcium intake during growth is a global public health concern. The aim of this study was to investigate the effects of dietary menaquinone-7 (MK-7) on bone accrual in growing Sprague–Dawley rats under calcium restriction. Following 13 weeks of treatment, various bone quality parameters...
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MDPI AG
2023-07-01
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| Online Access: | https://www.mdpi.com/2072-6643/15/15/3398 |
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| author | Ya Yuan Ignatius Man-Yau Szeto Na Li Hua Yang Yunzheng Zhou Biao Liu Fang He Lishi Zhang Sufang Duan Jinyao Chen |
| author_facet | Ya Yuan Ignatius Man-Yau Szeto Na Li Hua Yang Yunzheng Zhou Biao Liu Fang He Lishi Zhang Sufang Duan Jinyao Chen |
| author_sort | Ya Yuan |
| collection | DOAJ |
| description | Insufficient calcium intake during growth is a global public health concern. The aim of this study was to investigate the effects of dietary menaquinone-7 (MK-7) on bone accrual in growing Sprague–Dawley rats under calcium restriction. Following 13 weeks of treatment, various bone quality parameters, including microarchitecture, were measured. Fecal and cecal samples were subjected to microbiome (16S rRNA gene sequencing) analyses, while metabolomics analysis of the cecum and humerus samples was analyzed based on UHPLC-Q/TOF-MS. We found that calcium deficiency diminished the richness of the microbiome and disrupted microbiome composition, accompanied by an elevation in the relative abundance of <i>Parasutterella</i>. Furthermore, calcium insufficiency escalated the level of isovaleric acid and modified the metabolic profiles. MK-7 supplementation significantly increased the cortical thickness, cortical bone area, and the calcium content of the femur. Apart from improving bone calcium deposition and diminishing bone resorption, the mechanisms underlying the beneficial effects of MK on bone quality also involve the modulation of the host’s metabolic pathways and the composition of gut microbiota. The gut–bone axis holds promise as an efficacious target for ameliorating calcium deficiency in children’s bone quality, and MK-7 is a promising dietary supplement from this perspective. |
| first_indexed | 2024-03-11T00:19:56Z |
| format | Article |
| id | doaj.art-cfda4d079c9b49ebbc1518c03ab5d448 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T00:19:56Z |
| publishDate | 2023-07-01 |
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| spelling | doaj.art-cfda4d079c9b49ebbc1518c03ab5d4482023-11-18T23:24:01ZengMDPI AGNutrients2072-66432023-07-011515339810.3390/nu15153398Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-MetabolomicsYa Yuan0Ignatius Man-Yau Szeto1Na Li2Hua Yang3Yunzheng Zhou4Biao Liu5Fang He6Lishi Zhang7Sufang Duan8Jinyao Chen9Department of Nutrition and Food Safety, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaYili Maternal and Infant Nutrition Institute (YMINI), Inner Mongolia Yili Industrial Group, Co., Ltd., Beijing 100071, ChinaDepartment of Nutrition and Food Safety, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaThe Analysis and Assay Center of Sichuan University West China School of Public Health, Sichuan University, Chengdu 610093, ChinaDepartment of Nutrition and Food Safety, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaYili Maternal and Infant Nutrition Institute (YMINI), Inner Mongolia Yili Industrial Group, Co., Ltd., Beijing 100071, ChinaDepartment of Nutrition and Food Safety, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Nutrition and Food Safety, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaYili Maternal and Infant Nutrition Institute (YMINI), Inner Mongolia Yili Industrial Group, Co., Ltd., Beijing 100071, ChinaDepartment of Nutrition and Food Safety, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaInsufficient calcium intake during growth is a global public health concern. The aim of this study was to investigate the effects of dietary menaquinone-7 (MK-7) on bone accrual in growing Sprague–Dawley rats under calcium restriction. Following 13 weeks of treatment, various bone quality parameters, including microarchitecture, were measured. Fecal and cecal samples were subjected to microbiome (16S rRNA gene sequencing) analyses, while metabolomics analysis of the cecum and humerus samples was analyzed based on UHPLC-Q/TOF-MS. We found that calcium deficiency diminished the richness of the microbiome and disrupted microbiome composition, accompanied by an elevation in the relative abundance of <i>Parasutterella</i>. Furthermore, calcium insufficiency escalated the level of isovaleric acid and modified the metabolic profiles. MK-7 supplementation significantly increased the cortical thickness, cortical bone area, and the calcium content of the femur. Apart from improving bone calcium deposition and diminishing bone resorption, the mechanisms underlying the beneficial effects of MK on bone quality also involve the modulation of the host’s metabolic pathways and the composition of gut microbiota. The gut–bone axis holds promise as an efficacious target for ameliorating calcium deficiency in children’s bone quality, and MK-7 is a promising dietary supplement from this perspective.https://www.mdpi.com/2072-6643/15/15/3398menaquinone-7calciumgrowing ratbone mass accumulation16s rRNA gene sequencingmetabolomics |
| spellingShingle | Ya Yuan Ignatius Man-Yau Szeto Na Li Hua Yang Yunzheng Zhou Biao Liu Fang He Lishi Zhang Sufang Duan Jinyao Chen Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-Metabolomics Nutrients menaquinone-7 calcium growing rat bone mass accumulation 16s rRNA gene sequencing metabolomics |
| title | Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-Metabolomics |
| title_full | Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-Metabolomics |
| title_fullStr | Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-Metabolomics |
| title_full_unstemmed | Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-Metabolomics |
| title_short | Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-Metabolomics |
| title_sort | effects of menaquinone 7 on the bone health of growing rats under calcium restriction new insights from microbiome metabolomics |
| topic | menaquinone-7 calcium growing rat bone mass accumulation 16s rRNA gene sequencing metabolomics |
| url | https://www.mdpi.com/2072-6643/15/15/3398 |
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