| Summary: | Pathogens and mycotoxins are serious public health risks for humans and food safety in milk. This study concentrated on detecting <i>Staphylococcus aureus</i> and Ochratoxin A (OTA) in 210 pasteurized milk from ten urban Beijing districts to suggest the co-occurrence of <i>S. aureus</i> with toxin-producing genes and OTA in milk and the possible risk. <i>S. aureus</i> was identified by physiological and biochemical experiments and molecular biology experiments, and enterotoxin genes were identified by PCR. OTA was detected by LC-MS/MS. The study found 29 isolates of <i>S. aureus</i>, of which 17.24% had the sea gene encoding enterotoxin A. OTA was detected in 31 out of 120 samples and the maximum amount of detection was 18.8 μg/kg. The results of this study indicate that when failing to guarantee the cold chain, the presence of <i>S. aureus</i> with enterotoxin genes in milk will present a risk to food safety. Furthermore, the high detection rates and levels of OTA in milk suggest that OTA is a hidden risk. The co-occurrence of <i>S. aureus</i> and OTA in milk is a food safety concern and there is a need to control the occurrence of these two biohazards in milk.
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