Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (U...
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PeerJ Inc.
2021-11-01
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| Online Access: | https://peerj.com/articles/12513.pdf |
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| author | Jin-Yi Wan Jing-Xuan Wan Shilei Wang Xiaolu Wang Wenqian Guo Han Ma Yuqi Wu Chong-Zhi Wang Lian-Wen Qi Ping Li Haiqiang Yao Chun-Su Yuan |
| author_facet | Jin-Yi Wan Jing-Xuan Wan Shilei Wang Xiaolu Wang Wenqian Guo Han Ma Yuqi Wu Chong-Zhi Wang Lian-Wen Qi Ping Li Haiqiang Yao Chun-Su Yuan |
| author_sort | Jin-Yi Wan |
| collection | DOAJ |
| description | Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus. |
| first_indexed | 2024-03-09T06:58:30Z |
| format | Article |
| id | doaj.art-cfe184d7b04248d191b44f8739578246 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:58:30Z |
| publishDate | 2021-11-01 |
| publisher | PeerJ Inc. |
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| series | PeerJ |
| spelling | doaj.art-cfe184d7b04248d191b44f87395782462023-12-03T09:58:56ZengPeerJ Inc.PeerJ2167-83592021-11-019e1251310.7717/peerj.12513Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiotaJin-Yi Wan0Jing-Xuan Wan1Shilei Wang2Xiaolu Wang3Wenqian Guo4Han Ma5Yuqi Wu6Chong-Zhi Wang7Lian-Wen Qi8Ping Li9Haiqiang Yao10Chun-Su Yuan11School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, ChinaSchool of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, ChinaTang Center for Herbal Medicine Research & Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USAState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, ChinaSchool of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, ChinaTang Center for Herbal Medicine Research & Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USAOplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.https://peerj.com/articles/12513.pdfOplopanax elatusIntestinal microbiotaUPLC-Q-TOF/MSMetabolic profilesBiotransformation |
| spellingShingle | Jin-Yi Wan Jing-Xuan Wan Shilei Wang Xiaolu Wang Wenqian Guo Han Ma Yuqi Wu Chong-Zhi Wang Lian-Wen Qi Ping Li Haiqiang Yao Chun-Su Yuan Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota PeerJ Oplopanax elatus Intestinal microbiota UPLC-Q-TOF/MS Metabolic profiles Biotransformation |
| title | Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota |
| title_full | Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota |
| title_fullStr | Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota |
| title_full_unstemmed | Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota |
| title_short | Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota |
| title_sort | chemical profiling of root bark extract from oplopanax elatus and its in vitro biotransformation by human intestinal microbiota |
| topic | Oplopanax elatus Intestinal microbiota UPLC-Q-TOF/MS Metabolic profiles Biotransformation |
| url | https://peerj.com/articles/12513.pdf |
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