<i>Phoenix dactylifera</i> (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the Brain
Traditional medicine claims that various components of the <i>Phoenix dactylifera</i> (date plant) can be used to treat memory loss, fever, inflammation, loss of consciousness, and nerve disorders. The present study aims to evaluate the effectiveness of <i>Phoenix dactylifera</i...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/10413 |
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| author | Thippeswamy Boreddy Shivanandappa Ghallab Alotaibi Maheswari Chinnadhurai Sudharshan Reddy Dachani Mahmad Dabeer Ahmad Khalid Abdullah Aldaajanii |
| author_facet | Thippeswamy Boreddy Shivanandappa Ghallab Alotaibi Maheswari Chinnadhurai Sudharshan Reddy Dachani Mahmad Dabeer Ahmad Khalid Abdullah Aldaajanii |
| author_sort | Thippeswamy Boreddy Shivanandappa |
| collection | DOAJ |
| description | Traditional medicine claims that various components of the <i>Phoenix dactylifera</i> (date plant) can be used to treat memory loss, fever, inflammation, loss of consciousness, and nerve disorders. The present study aims to evaluate the effectiveness of <i>Phoenix dactylifera</i> fruit extracts (PDF) against rat sickness behaviour caused by lipopolysaccharide (LPS) by assessing behavioural and biochemical parameters. PDF was prepared by extracting dry fruits of <i>P. dactylifera</i> with a methanol:water (4:1, <i>v</i>/<i>v</i>) mixture. The PDF was evaluated for phenolic and flavonoid content and HPLC analysis of quercetin estimation. Adult Wistar rats were treated with LPS, PDF + LPS and dexamethasone + LPS. Water and food intake, behavioural tests such as locomotor activity, tail suspension and forced swim tests were conducted. Furthermore, alanine transaminase (ALT) and aspartate transaminase (AST) were estimated in plasma and malondialdehyde (MDA), reduced glutathione (GSH), nitrite, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), were estimated in the brain. PDF ameliorated LPS-induced sickness behaviour by reducing MDA, nitrite, IL-6, and TNF-α levels and improving GSH, behavioural alteration, water and food intake in the treated rats. In the plasma of the treated rats, PDF also decreased the levels of ALT and AST. The outcomes demonstrated the efficacy of PDF in reducing the sickness behaviour caused by LPS in rats. The authors believe that this study will provide the groundwork for future research to better understand the underlying mechanisms of action and therapeutic efficacy. |
| first_indexed | 2024-03-11T01:40:25Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:40:25Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cfedc08d258841c091b339699f192d132023-11-18T16:37:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124131041310.3390/ijms241310413<i>Phoenix dactylifera</i> (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the BrainThippeswamy Boreddy Shivanandappa0Ghallab Alotaibi1Maheswari Chinnadhurai2Sudharshan Reddy Dachani3Mahmad Dabeer Ahmad4Khalid Abdullah Aldaajanii5Department of Biomedical Science, College of Pharmacy, Shaqra University, Al-Dawadmi Campus, Al-Dawadmi 11961, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Shaqra University, Al-Dawadmi Campus, Al-Dawadmi 11961, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, Shaqra University, Al-Dawadmi Campus, Al-Dawadmi 11961, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, Shaqra University, Al-Dawadmi Campus, Al-Dawadmi 11961, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Shaqra University, Al-Dawadmi Campus, Al-Dawadmi 11961, Saudi ArabiaDepartment of Biomedical Science, College of Pharmacy, Shaqra University, Al-Dawadmi Campus, Al-Dawadmi 11961, Saudi ArabiaTraditional medicine claims that various components of the <i>Phoenix dactylifera</i> (date plant) can be used to treat memory loss, fever, inflammation, loss of consciousness, and nerve disorders. The present study aims to evaluate the effectiveness of <i>Phoenix dactylifera</i> fruit extracts (PDF) against rat sickness behaviour caused by lipopolysaccharide (LPS) by assessing behavioural and biochemical parameters. PDF was prepared by extracting dry fruits of <i>P. dactylifera</i> with a methanol:water (4:1, <i>v</i>/<i>v</i>) mixture. The PDF was evaluated for phenolic and flavonoid content and HPLC analysis of quercetin estimation. Adult Wistar rats were treated with LPS, PDF + LPS and dexamethasone + LPS. Water and food intake, behavioural tests such as locomotor activity, tail suspension and forced swim tests were conducted. Furthermore, alanine transaminase (ALT) and aspartate transaminase (AST) were estimated in plasma and malondialdehyde (MDA), reduced glutathione (GSH), nitrite, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), were estimated in the brain. PDF ameliorated LPS-induced sickness behaviour by reducing MDA, nitrite, IL-6, and TNF-α levels and improving GSH, behavioural alteration, water and food intake in the treated rats. In the plasma of the treated rats, PDF also decreased the levels of ALT and AST. The outcomes demonstrated the efficacy of PDF in reducing the sickness behaviour caused by LPS in rats. The authors believe that this study will provide the groundwork for future research to better understand the underlying mechanisms of action and therapeutic efficacy.https://www.mdpi.com/1422-0067/24/13/10413Ajwa date fruitssickness behaviourantioxidantsquercetincytokines |
| spellingShingle | Thippeswamy Boreddy Shivanandappa Ghallab Alotaibi Maheswari Chinnadhurai Sudharshan Reddy Dachani Mahmad Dabeer Ahmad Khalid Abdullah Aldaajanii <i>Phoenix dactylifera</i> (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the Brain International Journal of Molecular Sciences Ajwa date fruits sickness behaviour antioxidants quercetin cytokines |
| title | <i>Phoenix dactylifera</i> (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the Brain |
| title_full | <i>Phoenix dactylifera</i> (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the Brain |
| title_fullStr | <i>Phoenix dactylifera</i> (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the Brain |
| title_full_unstemmed | <i>Phoenix dactylifera</i> (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the Brain |
| title_short | <i>Phoenix dactylifera</i> (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the Brain |
| title_sort | i phoenix dactylifera i ajwa dates alleviate lps induced sickness behaviour in rats by attenuating proinflammatory cytokines and oxidative stress in the brain |
| topic | Ajwa date fruits sickness behaviour antioxidants quercetin cytokines |
| url | https://www.mdpi.com/1422-0067/24/13/10413 |
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