Deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population.
A frightening resurgence of bed bug infestations has occurred over the last 10 years in the U.S. and current chemical methods have been inadequate for controlling this pest due to widespread insecticide resistance. Little is known about the mechanisms of resistance present in U.S. bed bug population...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039447/pdf/?tool=EBI |
| _version_ | 1818404692167753728 |
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| author | Zach N Adelman Kathleen A Kilcullen Reina Koganemaru Michelle A E Anderson Troy D Anderson Dini M Miller |
| author_facet | Zach N Adelman Kathleen A Kilcullen Reina Koganemaru Michelle A E Anderson Troy D Anderson Dini M Miller |
| author_sort | Zach N Adelman |
| collection | DOAJ |
| description | A frightening resurgence of bed bug infestations has occurred over the last 10 years in the U.S. and current chemical methods have been inadequate for controlling this pest due to widespread insecticide resistance. Little is known about the mechanisms of resistance present in U.S. bed bug populations, making it extremely difficult to develop intelligent strategies for their control. We have identified bed bugs collected in Richmond, VA which exhibit both kdr-type (L925I) and metabolic resistance to pyrethroid insecticides. Using LD(50) bioassays, we determined that resistance ratios for Richmond strain bed bugs were ∼5200-fold to the insecticide deltamethrin. To identify metabolic genes potentially involved in the detoxification of pyrethroids, we performed deep-sequencing of the adult bed bug transcriptome, obtaining more than 2.5 million reads on the 454 titanium platform. Following assembly, analysis of newly identified gene transcripts in both Harlan (susceptible) and Richmond (resistant) bed bugs revealed several candidate cytochrome P450 and carboxylesterase genes which were significantly over-expressed in the resistant strain, consistent with the idea of increased metabolic resistance. These data will accelerate efforts to understand the biochemical basis for insecticide resistance in bed bugs, and provide molecular markers to assist in the surveillance of metabolic resistance. |
| first_indexed | 2024-12-14T08:44:11Z |
| format | Article |
| id | doaj.art-cfee0a120436422b99e87150b3f20805 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T08:44:11Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cfee0a120436422b99e87150b3f208052022-12-21T23:09:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2622810.1371/journal.pone.0026228Deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population.Zach N AdelmanKathleen A KilcullenReina KoganemaruMichelle A E AndersonTroy D AndersonDini M MillerA frightening resurgence of bed bug infestations has occurred over the last 10 years in the U.S. and current chemical methods have been inadequate for controlling this pest due to widespread insecticide resistance. Little is known about the mechanisms of resistance present in U.S. bed bug populations, making it extremely difficult to develop intelligent strategies for their control. We have identified bed bugs collected in Richmond, VA which exhibit both kdr-type (L925I) and metabolic resistance to pyrethroid insecticides. Using LD(50) bioassays, we determined that resistance ratios for Richmond strain bed bugs were ∼5200-fold to the insecticide deltamethrin. To identify metabolic genes potentially involved in the detoxification of pyrethroids, we performed deep-sequencing of the adult bed bug transcriptome, obtaining more than 2.5 million reads on the 454 titanium platform. Following assembly, analysis of newly identified gene transcripts in both Harlan (susceptible) and Richmond (resistant) bed bugs revealed several candidate cytochrome P450 and carboxylesterase genes which were significantly over-expressed in the resistant strain, consistent with the idea of increased metabolic resistance. These data will accelerate efforts to understand the biochemical basis for insecticide resistance in bed bugs, and provide molecular markers to assist in the surveillance of metabolic resistance.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039447/pdf/?tool=EBI |
| spellingShingle | Zach N Adelman Kathleen A Kilcullen Reina Koganemaru Michelle A E Anderson Troy D Anderson Dini M Miller Deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population. PLoS ONE |
| title | Deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population. |
| title_full | Deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population. |
| title_fullStr | Deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population. |
| title_full_unstemmed | Deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population. |
| title_short | Deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population. |
| title_sort | deep sequencing of pyrethroid resistant bed bugs reveals multiple mechanisms of resistance within a single population |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039447/pdf/?tool=EBI |
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