Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions
The aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil™ DNA isolation kit). The experiment based on dry rot fungus (Serpula lac...
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| Format: | Article |
| Language: | English |
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Mendel University Press
2008-01-01
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| Series: | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| Subjects: | |
| Online Access: | https://acta.mendelu.cz/56/2/0281/ |
| _version_ | 1818314093089521664 |
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| author | Elena Bobeková Michal Tomšovský Petr Horáček |
| author_facet | Elena Bobeková Michal Tomšovský Petr Horáček |
| author_sort | Elena Bobeková |
| collection | DOAJ |
| description | The aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil™ DNA isolation kit). The experiment based on dry rot fungus (Serpula lacrymans) detection from inoculated wooden pieces under laboratory conditions was followed by field detection of wood-decaying fungi from wood structures on building constructions. Fungal DNA was identified using the PCR–based methods including species-specific PCR and sequencing of amplified ITS region of ribosomal DNA. |
| first_indexed | 2024-12-13T08:44:09Z |
| format | Article |
| id | doaj.art-cfefbc1310914300bfa6a09979d50598 |
| institution | Directory Open Access Journal |
| issn | 1211-8516 2464-8310 |
| language | English |
| last_indexed | 2024-12-13T08:44:09Z |
| publishDate | 2008-01-01 |
| publisher | Mendel University Press |
| record_format | Article |
| series | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| spelling | doaj.art-cfefbc1310914300bfa6a09979d505982022-12-21T23:53:28ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102008-01-0156228128410.11118/actaun200856020281Application of molecular genetic methods for identification of wood-decaying fungi in wood constructionsElena Bobeková0Michal Tomšovský1Petr Horáček2Gagarinova 648/23, 96 223 Očová, Slovenská republikaÚstav ochrany lesů a myslivosti, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 3, 613 00 Brno, Česká republikaÚstav nauky o dřevě, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 3, 613 00 Brno, Česká republikaThe aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil™ DNA isolation kit). The experiment based on dry rot fungus (Serpula lacrymans) detection from inoculated wooden pieces under laboratory conditions was followed by field detection of wood-decaying fungi from wood structures on building constructions. Fungal DNA was identified using the PCR–based methods including species-specific PCR and sequencing of amplified ITS region of ribosomal DNA.https://acta.mendelu.cz/56/2/0281/woodfungi<i>Serpula lacrymans</i><i>Donkioporia expansa</i><i>Amylostereum areolatum</i><i>Cladosporium oxysporium</i> |
| spellingShingle | Elena Bobeková Michal Tomšovský Petr Horáček Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis wood fungi <i>Serpula lacrymans</i> <i>Donkioporia expansa</i> <i>Amylostereum areolatum</i> <i>Cladosporium oxysporium</i> |
| title | Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions |
| title_full | Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions |
| title_fullStr | Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions |
| title_full_unstemmed | Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions |
| title_short | Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions |
| title_sort | application of molecular genetic methods for identification of wood decaying fungi in wood constructions |
| topic | wood fungi <i>Serpula lacrymans</i> <i>Donkioporia expansa</i> <i>Amylostereum areolatum</i> <i>Cladosporium oxysporium</i> |
| url | https://acta.mendelu.cz/56/2/0281/ |
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