Role of ZmGST Gene Family Involved in Nicosulfuron Stress Tolerance Revealed by Genomic and Transcriptomic Analyses
Glutathione S-transferases (GST) are a large family of polymorphous proteins that play important roles in herbicide detoxification and stress response. Nicosulfuron is the most broadly used herbicide in maize fields, and it can cause different injuries to maize varieties, but little is studied about...
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MDPI AG
2022-10-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/11/2598 |
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| author | Xiaomin Liu Dan Zhao Xian Xu Libing Yuan Bochui Zhao Binghua Li Xinli Guo Guiqi Wang |
| author_facet | Xiaomin Liu Dan Zhao Xian Xu Libing Yuan Bochui Zhao Binghua Li Xinli Guo Guiqi Wang |
| author_sort | Xiaomin Liu |
| collection | DOAJ |
| description | Glutathione S-transferases (GST) are a large family of polymorphous proteins that play important roles in herbicide detoxification and stress response. Nicosulfuron is the most broadly used herbicide in maize fields, and it can cause different injuries to maize varieties, but little is studied about the systemic and comprehensive GST gene family responding to nicosulfuron stress in maize. In this research, pre-treatment with glutathione S-transferase inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) increased nicosulfuron phytotoxicity to both sensitive and tolerant maize genotypes. A total of 55 <i>ZmGST</i> genes belonging to six major sub-classes were identified in the maize genome and named according to the nomenclature system. Based on phylogenetic analyses, highly conserved gene structure and motif distribution were detected in the same class. Chromosome mapping results showed that <i>ZmGST</i> genes were distributed over the 10 chromosomes unevenly. There were thirteen and eight gene pairs identified as tandem and segmental duplication events, respectively, which played important roles in the expansion of the GST gene family in maize. RNA-seq and qRT-PCR analyses showed that there were great dissimilarities in <i>ZmGST</i> gene expression patterns between the tolerant and sensitive maize plants. More highly expressed <i>ZmGST</i> genes were found in the tolerant than in the sensitive without nicosulfuron stress. However, under 60 g a.i. ha<sup>−1</sup> nicosulfuron stress, more <i>ZmGST</i> genes were significantly upregulated in HB41 than in HB09. This study provided experimental evidence showing that glutathione S-transferases were involved in nicosulfuron stress in maize. It will contribute to the further functional analysis of the GST gene family in maize. |
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| language | English |
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| publishDate | 2022-10-01 |
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| series | Agronomy |
| spelling | doaj.art-cff0b3d3c6ab482b9406b1648aae66892023-11-24T03:18:58ZengMDPI AGAgronomy2073-43952022-10-011211259810.3390/agronomy12112598Role of ZmGST Gene Family Involved in Nicosulfuron Stress Tolerance Revealed by Genomic and Transcriptomic AnalysesXiaomin Liu0Dan Zhao1Xian Xu2Libing Yuan3Bochui Zhao4Binghua Li5Xinli Guo6Guiqi Wang7Key Laboratory of Crop Cultivation Physiology and Green Production of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, ChinaBiological Control Centre of Plant Pathogens and Plant Pests of Hebei Province, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, ChinaKey Laboratory of Crop Cultivation Physiology and Green Production of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, ChinaPlant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071001, ChinaKey Laboratory of Crop Cultivation Physiology and Green Production of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, ChinaKey Laboratory of Crop Cultivation Physiology and Green Production of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, ChinaKey Laboratory of Crop Cultivation Physiology and Green Production of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, ChinaKey Laboratory of Crop Cultivation Physiology and Green Production of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, ChinaGlutathione S-transferases (GST) are a large family of polymorphous proteins that play important roles in herbicide detoxification and stress response. Nicosulfuron is the most broadly used herbicide in maize fields, and it can cause different injuries to maize varieties, but little is studied about the systemic and comprehensive GST gene family responding to nicosulfuron stress in maize. In this research, pre-treatment with glutathione S-transferase inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) increased nicosulfuron phytotoxicity to both sensitive and tolerant maize genotypes. A total of 55 <i>ZmGST</i> genes belonging to six major sub-classes were identified in the maize genome and named according to the nomenclature system. Based on phylogenetic analyses, highly conserved gene structure and motif distribution were detected in the same class. Chromosome mapping results showed that <i>ZmGST</i> genes were distributed over the 10 chromosomes unevenly. There were thirteen and eight gene pairs identified as tandem and segmental duplication events, respectively, which played important roles in the expansion of the GST gene family in maize. RNA-seq and qRT-PCR analyses showed that there were great dissimilarities in <i>ZmGST</i> gene expression patterns between the tolerant and sensitive maize plants. More highly expressed <i>ZmGST</i> genes were found in the tolerant than in the sensitive without nicosulfuron stress. However, under 60 g a.i. ha<sup>−1</sup> nicosulfuron stress, more <i>ZmGST</i> genes were significantly upregulated in HB41 than in HB09. This study provided experimental evidence showing that glutathione S-transferases were involved in nicosulfuron stress in maize. It will contribute to the further functional analysis of the GST gene family in maize.https://www.mdpi.com/2073-4395/12/11/2598glutathione S-transferases (GST)<i>Zea mays</i> L.nicosulfurongenome-wide analysisherbicide stress |
| spellingShingle | Xiaomin Liu Dan Zhao Xian Xu Libing Yuan Bochui Zhao Binghua Li Xinli Guo Guiqi Wang Role of ZmGST Gene Family Involved in Nicosulfuron Stress Tolerance Revealed by Genomic and Transcriptomic Analyses Agronomy glutathione S-transferases (GST) <i>Zea mays</i> L. nicosulfuron genome-wide analysis herbicide stress |
| title | Role of ZmGST Gene Family Involved in Nicosulfuron Stress Tolerance Revealed by Genomic and Transcriptomic Analyses |
| title_full | Role of ZmGST Gene Family Involved in Nicosulfuron Stress Tolerance Revealed by Genomic and Transcriptomic Analyses |
| title_fullStr | Role of ZmGST Gene Family Involved in Nicosulfuron Stress Tolerance Revealed by Genomic and Transcriptomic Analyses |
| title_full_unstemmed | Role of ZmGST Gene Family Involved in Nicosulfuron Stress Tolerance Revealed by Genomic and Transcriptomic Analyses |
| title_short | Role of ZmGST Gene Family Involved in Nicosulfuron Stress Tolerance Revealed by Genomic and Transcriptomic Analyses |
| title_sort | role of zmgst gene family involved in nicosulfuron stress tolerance revealed by genomic and transcriptomic analyses |
| topic | glutathione S-transferases (GST) <i>Zea mays</i> L. nicosulfuron genome-wide analysis herbicide stress |
| url | https://www.mdpi.com/2073-4395/12/11/2598 |
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