A PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in China
Abstract Background Bovine babesiosis and theileriosis is an important hemoprotozoal disease in cattles and yaks in tropical and subtropical regions leading to significant economic losses. In the field, the risk of co-infection between the bovine Babesia and Theileria species is very high. Thus, it...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2015-09-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-015-1085-x |
| _version_ | 1797811847890468864 |
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| author | Zhancheng Tian Junzheng Du Jifei Yang Aihong Liu Xiaocui Liu Guangyuan Liu Hong Yin |
| author_facet | Zhancheng Tian Junzheng Du Jifei Yang Aihong Liu Xiaocui Liu Guangyuan Liu Hong Yin |
| author_sort | Zhancheng Tian |
| collection | DOAJ |
| description | Abstract Background Bovine babesiosis and theileriosis is an important hemoprotozoal disease in cattles and yaks in tropical and subtropical regions leading to significant economic losses. In the field, the risk of co-infection between the bovine Babesia and Theileria species is very high. Thus, it is necessary to develop a simple, accurate, rapid and cost-effective method for large-scale epidemic investigation, in particular for the detection of co-infection in field. Methods In this study, DNA sequences of a ribosomal protein S8 (RPS8) gene from eight species of cattle piroplasms in China were used to develop a species-specific PCR-RFLP diagnostic tool. The eight Theileria and Babesia species could be differentiated by digesting the RPS8 PCR product with Mbo I. Results The sensitivity of the PCR assays was 0.1 pg DNA for Babesia species but 1 pg DNA for Theileria species. The clearly different size of the PCR-RFLP products allowed for a direct discrimination between eight bovine Theileria and Babesia species (T. annulata, T. sinensis, T. sergenti, B. ovata, B. bovis, B. bigemina, B. major and Babesia species Kashi isolate). Conclusion Our results indicated that the established method based on the RPS8 gene was a reliable molecular diagnostic tool for the simultaneous detection and identification of bovine Babesia and Theileria species in China, which could be applicable for the survey of parasite dynamics, epidemiological studies as well as prevention and control of the disease. |
| first_indexed | 2024-03-13T07:29:51Z |
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| id | doaj.art-cff1a33c01c3491181e0c455b30838c8 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:51Z |
| publishDate | 2015-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-cff1a33c01c3491181e0c455b30838c82023-06-04T11:11:50ZengBMCParasites & Vectors1756-33052015-09-01811510.1186/s13071-015-1085-xA PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in ChinaZhancheng Tian0Junzheng Du1Jifei Yang2Aihong Liu3Xiaocui Liu4Guangyuan Liu5Hong Yin6State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesAbstract Background Bovine babesiosis and theileriosis is an important hemoprotozoal disease in cattles and yaks in tropical and subtropical regions leading to significant economic losses. In the field, the risk of co-infection between the bovine Babesia and Theileria species is very high. Thus, it is necessary to develop a simple, accurate, rapid and cost-effective method for large-scale epidemic investigation, in particular for the detection of co-infection in field. Methods In this study, DNA sequences of a ribosomal protein S8 (RPS8) gene from eight species of cattle piroplasms in China were used to develop a species-specific PCR-RFLP diagnostic tool. The eight Theileria and Babesia species could be differentiated by digesting the RPS8 PCR product with Mbo I. Results The sensitivity of the PCR assays was 0.1 pg DNA for Babesia species but 1 pg DNA for Theileria species. The clearly different size of the PCR-RFLP products allowed for a direct discrimination between eight bovine Theileria and Babesia species (T. annulata, T. sinensis, T. sergenti, B. ovata, B. bovis, B. bigemina, B. major and Babesia species Kashi isolate). Conclusion Our results indicated that the established method based on the RPS8 gene was a reliable molecular diagnostic tool for the simultaneous detection and identification of bovine Babesia and Theileria species in China, which could be applicable for the survey of parasite dynamics, epidemiological studies as well as prevention and control of the disease.https://doi.org/10.1186/s13071-015-1085-xBovine Babesia and Theileria speciesRibosomal protein S8DiscriminationPCR-RFLP |
| spellingShingle | Zhancheng Tian Junzheng Du Jifei Yang Aihong Liu Xiaocui Liu Guangyuan Liu Hong Yin A PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in China Parasites & Vectors Bovine Babesia and Theileria species Ribosomal protein S8 Discrimination PCR-RFLP |
| title | A PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in China |
| title_full | A PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in China |
| title_fullStr | A PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in China |
| title_full_unstemmed | A PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in China |
| title_short | A PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in China |
| title_sort | pcr rflp assay targeting rps8 gene for the discrimination between bovine babesia and theileria species in china |
| topic | Bovine Babesia and Theileria species Ribosomal protein S8 Discrimination PCR-RFLP |
| url | https://doi.org/10.1186/s13071-015-1085-x |
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