Time-restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigs
IntroductionStudies demonstrate that time-restricted feeding (TRF) can regulate gut microbiota composition. However, it is unclear whether TRF could affect the gut microbial rhythmicity in growing pigs. Therefore, the present study aimed to explore the effects of TRF on the dynamic fluctuation of th...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1162482/full |
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| author | Hongyu Wang Hongyu Wang Qiuke Li Qiuke Li Rongying Xu Rongying Xu Yong Su Yong Su Weiyun Zhu Weiyun Zhu |
| author_facet | Hongyu Wang Hongyu Wang Qiuke Li Qiuke Li Rongying Xu Rongying Xu Yong Su Yong Su Weiyun Zhu Weiyun Zhu |
| author_sort | Hongyu Wang |
| collection | DOAJ |
| description | IntroductionStudies demonstrate that time-restricted feeding (TRF) can regulate gut microbiota composition. However, it is unclear whether TRF could affect the gut microbial rhythmicity in growing pigs. Therefore, the present study aimed to explore the effects of TRF on the dynamic fluctuation of the gut microbiota.MethodsA total of 10 healthy growing pigs equipped with T cannula were employed. Pigs were randomly allotted to the free access (FA) and the TRF groups with 5 replicates (1 pig/replicates). Pigs in the FA group were fed free access during the whole experimental period, whereas pigs in the TRF group were fed free access three times per day within limited times (7:00–8:00, 12:00–13:00, 17:00–18:00). The experiment lasted for 15 days, at 06:00 a.m. of the day 16, colonic digesta were collected at a 6-h interval for consecutive 24 h marked as T06 (06:00), T12 (12:00), T18 (18:00), T24 (24:00), T30 (06:00), respectively.ResultsResults showed that TRF altered the distribution of feed intake without changing the total feed intake within a day (p = 0.870). TRF decreased the overall concentration of colonic cellulose and altered their oscillating patterns. All alpha-diversity indexes of different time points showed significant differences regardless of feeding pattern with a trough at T18 or T24. TRF shifted the trough of the alpha-diversity index Simpson and Invsimpson. TRF lost the rhythmicity of Prevotellaceae, Ruminococcaceae, Bacteroidales_S24-7_group, and Peptococcaceae and gained the rhythmicity of Pasteurellaceae, Clostridiaceae_1, Veillonellaceae, and Peptostreptococcaceae. Also, TRF altered the interaction pattern by increasing the microbes involved in the co-occurrence network and their crosstalk, especially at T24. Interestingly, the microbial variation at T24 could largely explained by colonic substrates starch (R2 = 0.369; p = 0.001), cellulose (R2 = 0.235; p = 0.009) and NH4-N (R2 = 0.489; p = 0.001).ConclusionIn conclusion, TRF has changed the concentrates of cellulose and the relative abundance of specific microbes and certain microbial metabolites. In addition, TRF has more powerful effects on the fluctuation modes of these nutrient substrates, microbes, and metabolites by shifting their peaks or troughs. This knowledge facilitates the development of precision regulation targeting gut microbial rhythmicity. |
| first_indexed | 2024-03-13T10:27:10Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T10:27:10Z |
| publishDate | 2023-05-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-cff242d656d34af2a0da83737dcaa7b42023-05-19T05:29:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11624821162482Time-restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigsHongyu Wang0Hongyu Wang1Qiuke Li2Qiuke Li3Rongying Xu4Rongying Xu5Yong Su6Yong Su7Weiyun Zhu8Weiyun Zhu9Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaIntroductionStudies demonstrate that time-restricted feeding (TRF) can regulate gut microbiota composition. However, it is unclear whether TRF could affect the gut microbial rhythmicity in growing pigs. Therefore, the present study aimed to explore the effects of TRF on the dynamic fluctuation of the gut microbiota.MethodsA total of 10 healthy growing pigs equipped with T cannula were employed. Pigs were randomly allotted to the free access (FA) and the TRF groups with 5 replicates (1 pig/replicates). Pigs in the FA group were fed free access during the whole experimental period, whereas pigs in the TRF group were fed free access three times per day within limited times (7:00–8:00, 12:00–13:00, 17:00–18:00). The experiment lasted for 15 days, at 06:00 a.m. of the day 16, colonic digesta were collected at a 6-h interval for consecutive 24 h marked as T06 (06:00), T12 (12:00), T18 (18:00), T24 (24:00), T30 (06:00), respectively.ResultsResults showed that TRF altered the distribution of feed intake without changing the total feed intake within a day (p = 0.870). TRF decreased the overall concentration of colonic cellulose and altered their oscillating patterns. All alpha-diversity indexes of different time points showed significant differences regardless of feeding pattern with a trough at T18 or T24. TRF shifted the trough of the alpha-diversity index Simpson and Invsimpson. TRF lost the rhythmicity of Prevotellaceae, Ruminococcaceae, Bacteroidales_S24-7_group, and Peptococcaceae and gained the rhythmicity of Pasteurellaceae, Clostridiaceae_1, Veillonellaceae, and Peptostreptococcaceae. Also, TRF altered the interaction pattern by increasing the microbes involved in the co-occurrence network and their crosstalk, especially at T24. Interestingly, the microbial variation at T24 could largely explained by colonic substrates starch (R2 = 0.369; p = 0.001), cellulose (R2 = 0.235; p = 0.009) and NH4-N (R2 = 0.489; p = 0.001).ConclusionIn conclusion, TRF has changed the concentrates of cellulose and the relative abundance of specific microbes and certain microbial metabolites. In addition, TRF has more powerful effects on the fluctuation modes of these nutrient substrates, microbes, and metabolites by shifting their peaks or troughs. This knowledge facilitates the development of precision regulation targeting gut microbial rhythmicity.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1162482/fullgut microbiotagrowing pigstime-restricted feedingmicrobial rhythmicity16S rRNA sequencing |
| spellingShingle | Hongyu Wang Hongyu Wang Qiuke Li Qiuke Li Rongying Xu Rongying Xu Yong Su Yong Su Weiyun Zhu Weiyun Zhu Time-restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigs Frontiers in Microbiology gut microbiota growing pigs time-restricted feeding microbial rhythmicity 16S rRNA sequencing |
| title | Time-restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigs |
| title_full | Time-restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigs |
| title_fullStr | Time-restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigs |
| title_full_unstemmed | Time-restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigs |
| title_short | Time-restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigs |
| title_sort | time restricted feeding affects colonic nutrient substrates and modulates the diurnal fluctuation of microbiota in pigs |
| topic | gut microbiota growing pigs time-restricted feeding microbial rhythmicity 16S rRNA sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1162482/full |
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