Nano Polyamidoamine-G7 (NPAMAM-G7) Dendrimer Synthesis and Assessment the Effect of its antibacterial on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution
Background: Nano scale dendrimers are macromolecules synthetic which frequently used in medical and health field. This study was aimed to examine synthesis and the antibacterial effect of NanoPolyamidoamine-G7 (NPAMAM-G7) dendrimer on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Pr...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2016-06-01
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| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://tumj.tums.ac.ir/browse.php?a_code=A-10-2143-2&slc_lang=en&sid=1 |
| _version_ | 1818984818642255872 |
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| author | mitra Gholami Shahram Nazari Mahdi Farzadkia Gharib Majidi Soudabeh Alizadeh Matboo |
| author_facet | mitra Gholami Shahram Nazari Mahdi Farzadkia Gharib Majidi Soudabeh Alizadeh Matboo |
| author_sort | mitra Gholami |
| collection | DOAJ |
| description | Background: Nano scale dendrimers are macromolecules synthetic which frequently used in medical and health field. This study was aimed to examine synthesis and the antibacterial effect of NanoPolyamidoamine-G7 (NPAMAM-G7) dendrimer on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution.
Material and methods: In this experimental study, initially dilution of 103 CFU/ml were prepared from each strain of bacteria. Then different concentrations of dendrimer (0.025, 0.25, 2.5 and 25 µg/ml) in the laboratory temperature (23-25 °C) was added to water. In order to determine the efficiency of dendrimers in removal of bacteria, samples were taken at different times (0, 10, 20, 30, 40, 50 and 60 min) and were cultured on nutrient agar medium. Samples were incubated for 24 hours at 37 °C and then number of colonies were counted.
Results: Antibacterial properties of dendrimers in aqueous solution by increasing the dendrimer concentration and contact time is directly related. At a concentration of 25 μg/ml at 60 minutes all bacteria except Staphylococcus Aureus, and at 30 minutes, Escherichia Coli and Klebsiella Oxytoca bacteria for 100% excluded. The concentration of 2.5 μg/ml at 60 minutes of bacteria, Escherichia Coli, Klebsiella Oxytoca and Proteus Mirabilis are 100% excluded. All concentrations of dendrimers at different times were reduced bacteria in the PAMAM- G7 dendrimer effect on gram-negative bacteria, gram-positive bacteria was better.
Conclusion: The NPAMAM-G7 dendrimer with end amine groups exhibited a positive impact on the removal of standard strains, gram-positive and gram-negative bacteria. Therefore, it is possible to use these nanodendrimers as antibacterial in the future. |
| first_indexed | 2024-12-20T18:24:58Z |
| format | Article |
| id | doaj.art-cff320dd51374360967f9e5a2152fa6d |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-20T18:24:58Z |
| publishDate | 2016-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-cff320dd51374360967f9e5a2152fa6d2022-12-21T19:30:11ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222016-06-0174300Nano Polyamidoamine-G7 (NPAMAM-G7) Dendrimer Synthesis and Assessment the Effect of its antibacterial on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solutionmitra Gholami0Shahram Nazari1Mahdi Farzadkia2Gharib Majidi3Soudabeh Alizadeh Matboo4 Background: Nano scale dendrimers are macromolecules synthetic which frequently used in medical and health field. This study was aimed to examine synthesis and the antibacterial effect of NanoPolyamidoamine-G7 (NPAMAM-G7) dendrimer on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution. Material and methods: In this experimental study, initially dilution of 103 CFU/ml were prepared from each strain of bacteria. Then different concentrations of dendrimer (0.025, 0.25, 2.5 and 25 µg/ml) in the laboratory temperature (23-25 °C) was added to water. In order to determine the efficiency of dendrimers in removal of bacteria, samples were taken at different times (0, 10, 20, 30, 40, 50 and 60 min) and were cultured on nutrient agar medium. Samples were incubated for 24 hours at 37 °C and then number of colonies were counted. Results: Antibacterial properties of dendrimers in aqueous solution by increasing the dendrimer concentration and contact time is directly related. At a concentration of 25 μg/ml at 60 minutes all bacteria except Staphylococcus Aureus, and at 30 minutes, Escherichia Coli and Klebsiella Oxytoca bacteria for 100% excluded. The concentration of 2.5 μg/ml at 60 minutes of bacteria, Escherichia Coli, Klebsiella Oxytoca and Proteus Mirabilis are 100% excluded. All concentrations of dendrimers at different times were reduced bacteria in the PAMAM- G7 dendrimer effect on gram-negative bacteria, gram-positive bacteria was better. Conclusion: The NPAMAM-G7 dendrimer with end amine groups exhibited a positive impact on the removal of standard strains, gram-positive and gram-negative bacteria. Therefore, it is possible to use these nanodendrimers as antibacterial in the future.http://tumj.tums.ac.ir/browse.php?a_code=A-10-2143-2&slc_lang=en&sid=1SynthesisNanoPolyamidoamine-G7 Aqueous solution Gram-negative and gram-positive bacteria |
| spellingShingle | mitra Gholami Shahram Nazari Mahdi Farzadkia Gharib Majidi Soudabeh Alizadeh Matboo Nano Polyamidoamine-G7 (NPAMAM-G7) Dendrimer Synthesis and Assessment the Effect of its antibacterial on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution Tehran University Medical Journal Synthesis NanoPolyamidoamine-G7 Aqueous solution Gram-negative and gram-positive bacteria |
| title | Nano Polyamidoamine-G7 (NPAMAM-G7) Dendrimer Synthesis and Assessment the Effect of its antibacterial on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution |
| title_full | Nano Polyamidoamine-G7 (NPAMAM-G7) Dendrimer Synthesis and Assessment the Effect of its antibacterial on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution |
| title_fullStr | Nano Polyamidoamine-G7 (NPAMAM-G7) Dendrimer Synthesis and Assessment the Effect of its antibacterial on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution |
| title_full_unstemmed | Nano Polyamidoamine-G7 (NPAMAM-G7) Dendrimer Synthesis and Assessment the Effect of its antibacterial on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution |
| title_short | Nano Polyamidoamine-G7 (NPAMAM-G7) Dendrimer Synthesis and Assessment the Effect of its antibacterial on Escherichia Coli, Klebsiella Oxytoca, Pseudomonas Aeruginosa , Proteus Mirabilis and Staphylococcus Aureus from aqueous solution |
| title_sort | nano polyamidoamine g7 npamam g7 dendrimer synthesis and assessment the effect of its antibacterial on escherichia coli klebsiella oxytoca pseudomonas aeruginosa proteus mirabilis and staphylococcus aureus from aqueous solution |
| topic | Synthesis NanoPolyamidoamine-G7 Aqueous solution Gram-negative and gram-positive bacteria |
| url | http://tumj.tums.ac.ir/browse.php?a_code=A-10-2143-2&slc_lang=en&sid=1 |
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