Transcriptome Analysis Reveals the Response Mechanism of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana</i> and <i>Poa annua</i> under 4,8-Dihydroxy-1-tetralone Treatment
4,8-dihydroxy-l-tetralone (4,8-DHT) is an allelochemical isolated from the outer bark of <i>Carya cathayensis</i> that acts as a plant growth inhibitor. In order to explore the mechanism of 4,8-DHT inhibiting weed activity, we treated three species of <i>Digitaria sanguinalis</i...
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/14/2728 |
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| author | Qiumin Sun Tao Wang Jiu Huang Xinyi Gu Yanling Dong Ying Yang Xiaowen Da Xiaorong Mo Xiaoting Xie Hangjin Jiang Daoliang Yan Bingsong Zheng Yi He |
| author_facet | Qiumin Sun Tao Wang Jiu Huang Xinyi Gu Yanling Dong Ying Yang Xiaowen Da Xiaorong Mo Xiaoting Xie Hangjin Jiang Daoliang Yan Bingsong Zheng Yi He |
| author_sort | Qiumin Sun |
| collection | DOAJ |
| description | 4,8-dihydroxy-l-tetralone (4,8-DHT) is an allelochemical isolated from the outer bark of <i>Carya cathayensis</i> that acts as a plant growth inhibitor. In order to explore the mechanism of 4,8-DHT inhibiting weed activity, we treated three species of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana,</i> and <i>Poa annua</i> with different concentrations of 4,8-DHT and performed phenotype observation and transcriptome sequencing. The results showed that with an increase in 4,8-DHT concentration, the degree of plant damage gradually deepened. Under the same concentration of 4,8-DHT, the damage degree of leaves and roots of <i>Digitaria sanguinalis</i> was the greatest, followed by <i>Arabidopsis thaliana</i>, while <i>Poa annua</i> had the least damage, and the leaves turned slightly yellow. Transcriptome data showed that 24536, 9913, and 1662 differentially expressed genes (DEGs) were identified in <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana,</i> and <i>Poa annua</i>, respectively. These DEGs were significantly enriched in photosynthesis, carbon fixation, glutathione metabolism, phenylpropanoid biosynthesis, and oxidative phosphorylation pathways. In addition, DEGs were also enriched in plant hormone signal transduction and the MAPK signal pathway in <i>Arabidopsis thaliana</i>. Further analysis showed that after 4,8-DHT treatment, the transcript levels of photosynthesis PSI- and PSII-related genes, LHCA/B-related genes, Rubisco, and PEPC were significantly decreased in <i>Digitaria sanguinalis</i> and <i>Arabidopsis thaliana</i>. At the same time, the transcription levels of genes related to glutathione metabolism and the phenylpropanoid biosynthesis pathway in <i>Digitaria sanguinalis</i> were also significantly decreased. However, the expression of these genes was upregulated in <i>Arabidopsis thaliana</i> and <i>Poa annua</i>. These indicated that 4,8-DHT affected the growth of the three plants through different physiological pathways, and then played a role in inhibiting plant growth. Simultaneously, the extent to which plants were affected depended on the tested plants and the content of 4,8-DHT. The identification of weed genes that respond to 4,8-DHT has helped us to further understand the inhibition of plant growth by allelochemicals and has provided a scientific basis for the development of allelochemicals as herbicides. |
| first_indexed | 2024-03-11T00:43:22Z |
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| spelling | doaj.art-cff4015d343a435993f469adeb578c0e2023-11-18T21:01:12ZengMDPI AGPlants2223-77472023-07-011214272810.3390/plants12142728Transcriptome Analysis Reveals the Response Mechanism of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana</i> and <i>Poa annua</i> under 4,8-Dihydroxy-1-tetralone TreatmentQiumin Sun0Tao Wang1Jiu Huang2Xinyi Gu3Yanling Dong4Ying Yang5Xiaowen Da6Xiaorong Mo7Xiaoting Xie8Hangjin Jiang9Daoliang Yan10Bingsong Zheng11Yi He12State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaSchool of Environment science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaCenter for Data Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China4,8-dihydroxy-l-tetralone (4,8-DHT) is an allelochemical isolated from the outer bark of <i>Carya cathayensis</i> that acts as a plant growth inhibitor. In order to explore the mechanism of 4,8-DHT inhibiting weed activity, we treated three species of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana,</i> and <i>Poa annua</i> with different concentrations of 4,8-DHT and performed phenotype observation and transcriptome sequencing. The results showed that with an increase in 4,8-DHT concentration, the degree of plant damage gradually deepened. Under the same concentration of 4,8-DHT, the damage degree of leaves and roots of <i>Digitaria sanguinalis</i> was the greatest, followed by <i>Arabidopsis thaliana</i>, while <i>Poa annua</i> had the least damage, and the leaves turned slightly yellow. Transcriptome data showed that 24536, 9913, and 1662 differentially expressed genes (DEGs) were identified in <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana,</i> and <i>Poa annua</i>, respectively. These DEGs were significantly enriched in photosynthesis, carbon fixation, glutathione metabolism, phenylpropanoid biosynthesis, and oxidative phosphorylation pathways. In addition, DEGs were also enriched in plant hormone signal transduction and the MAPK signal pathway in <i>Arabidopsis thaliana</i>. Further analysis showed that after 4,8-DHT treatment, the transcript levels of photosynthesis PSI- and PSII-related genes, LHCA/B-related genes, Rubisco, and PEPC were significantly decreased in <i>Digitaria sanguinalis</i> and <i>Arabidopsis thaliana</i>. At the same time, the transcription levels of genes related to glutathione metabolism and the phenylpropanoid biosynthesis pathway in <i>Digitaria sanguinalis</i> were also significantly decreased. However, the expression of these genes was upregulated in <i>Arabidopsis thaliana</i> and <i>Poa annua</i>. These indicated that 4,8-DHT affected the growth of the three plants through different physiological pathways, and then played a role in inhibiting plant growth. Simultaneously, the extent to which plants were affected depended on the tested plants and the content of 4,8-DHT. The identification of weed genes that respond to 4,8-DHT has helped us to further understand the inhibition of plant growth by allelochemicals and has provided a scientific basis for the development of allelochemicals as herbicides.https://www.mdpi.com/2223-7747/12/14/27284,8-DHTphotosynthesisantioxidant enzymesherbicides |
| spellingShingle | Qiumin Sun Tao Wang Jiu Huang Xinyi Gu Yanling Dong Ying Yang Xiaowen Da Xiaorong Mo Xiaoting Xie Hangjin Jiang Daoliang Yan Bingsong Zheng Yi He Transcriptome Analysis Reveals the Response Mechanism of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana</i> and <i>Poa annua</i> under 4,8-Dihydroxy-1-tetralone Treatment Plants 4,8-DHT photosynthesis antioxidant enzymes herbicides |
| title | Transcriptome Analysis Reveals the Response Mechanism of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana</i> and <i>Poa annua</i> under 4,8-Dihydroxy-1-tetralone Treatment |
| title_full | Transcriptome Analysis Reveals the Response Mechanism of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana</i> and <i>Poa annua</i> under 4,8-Dihydroxy-1-tetralone Treatment |
| title_fullStr | Transcriptome Analysis Reveals the Response Mechanism of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana</i> and <i>Poa annua</i> under 4,8-Dihydroxy-1-tetralone Treatment |
| title_full_unstemmed | Transcriptome Analysis Reveals the Response Mechanism of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana</i> and <i>Poa annua</i> under 4,8-Dihydroxy-1-tetralone Treatment |
| title_short | Transcriptome Analysis Reveals the Response Mechanism of <i>Digitaria sanguinalis</i>, <i>Arabidopsis thaliana</i> and <i>Poa annua</i> under 4,8-Dihydroxy-1-tetralone Treatment |
| title_sort | transcriptome analysis reveals the response mechanism of i digitaria sanguinalis i i arabidopsis thaliana i and i poa annua i under 4 8 dihydroxy 1 tetralone treatment |
| topic | 4,8-DHT photosynthesis antioxidant enzymes herbicides |
| url | https://www.mdpi.com/2223-7747/12/14/2728 |
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