Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer Cells
Theranostics of cancer using smart biocompatible materials can enable early cancer diagnostics and treatment. Here, we report on a DNA-nanoparticle functional material, which can simultaneously report the presence of an mRNA cancer biomarker and trigger its degradation in cultured cells. The nanodev...
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| Format: | Article |
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MDPI AG
2019-04-01
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| Series: | Magnetochemistry |
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| Online Access: | https://www.mdpi.com/2312-7481/5/2/24 |
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| author | Saira Bakshi Andrey Zakharchenko Sergiy Minko Dmitry M. Kolpashchikov Evgeny Katz |
| author_facet | Saira Bakshi Andrey Zakharchenko Sergiy Minko Dmitry M. Kolpashchikov Evgeny Katz |
| author_sort | Saira Bakshi |
| collection | DOAJ |
| description | Theranostics of cancer using smart biocompatible materials can enable early cancer diagnostics and treatment. Here, we report on a DNA-nanoparticle functional material, which can simultaneously report the presence of an mRNA cancer biomarker and trigger its degradation in cultured cells. The nanodevice consists of two species of magnetic beads, each of which is conjugated with different components of a multicomponent deoxyribozyme (DZ) sensor. The system is activated only under two conditions: (i) in the presence of a specific target mRNA and (ii) when a magnetic field is applied. We demonstrate that delivery of such a system is markedly enhanced by the application of a magnetic field. The system not only fluorescently detects target mRNA in cultured MCF-7 cancer cells, but also induces its downregulation. Thus, the two-component magnetic nanoparticle system has characteristics of a material that can be used for cancer theranostics. |
| first_indexed | 2024-12-18T08:21:31Z |
| format | Article |
| id | doaj.art-d00290ba62ad4b3aad4030540d570235 |
| institution | Directory Open Access Journal |
| issn | 2312-7481 |
| language | English |
| last_indexed | 2024-12-18T08:21:31Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Magnetochemistry |
| spelling | doaj.art-d00290ba62ad4b3aad4030540d5702352022-12-21T21:14:44ZengMDPI AGMagnetochemistry2312-74812019-04-01522410.3390/magnetochemistry5020024magnetochemistry5020024Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer CellsSaira Bakshi0Andrey Zakharchenko1Sergiy Minko2Dmitry M. Kolpashchikov3Evgeny Katz4Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USANanostructured Materials Lab, University of Georgia, Athens, GA 30602, USANanostructured Materials Lab, University of Georgia, Athens, GA 30602, USAChemistry Department, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816-2366, USADepartment of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USATheranostics of cancer using smart biocompatible materials can enable early cancer diagnostics and treatment. Here, we report on a DNA-nanoparticle functional material, which can simultaneously report the presence of an mRNA cancer biomarker and trigger its degradation in cultured cells. The nanodevice consists of two species of magnetic beads, each of which is conjugated with different components of a multicomponent deoxyribozyme (DZ) sensor. The system is activated only under two conditions: (i) in the presence of a specific target mRNA and (ii) when a magnetic field is applied. We demonstrate that delivery of such a system is markedly enhanced by the application of a magnetic field. The system not only fluorescently detects target mRNA in cultured MCF-7 cancer cells, but also induces its downregulation. Thus, the two-component magnetic nanoparticle system has characteristics of a material that can be used for cancer theranostics.https://www.mdpi.com/2312-7481/5/2/24RNA analysismagnetic nanoparticlestheranosticscancer biomarkerdeoxyribozymebiosensor |
| spellingShingle | Saira Bakshi Andrey Zakharchenko Sergiy Minko Dmitry M. Kolpashchikov Evgeny Katz Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer Cells Magnetochemistry RNA analysis magnetic nanoparticles theranostics cancer biomarker deoxyribozyme biosensor |
| title | Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer Cells |
| title_full | Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer Cells |
| title_fullStr | Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer Cells |
| title_full_unstemmed | Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer Cells |
| title_short | Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer Cells |
| title_sort | towards nanomaterials for cancer theranostics a system of dna modified magnetic nanoparticles for detection and suppression of rna marker in cancer cells |
| topic | RNA analysis magnetic nanoparticles theranostics cancer biomarker deoxyribozyme biosensor |
| url | https://www.mdpi.com/2312-7481/5/2/24 |
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