Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep
Rumen methanogens prevent the accumulation of fermentation gases in the rumen and generate methane that increases global warming and represents a loss in animals' gross energy. Non-traditional feed resources such as the by-products of date palm (Phoenix dactylifera) and olive (Olea europaea) tr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AIMS Press
2022-01-01
|
| Series: | AIMS Microbiology |
| Subjects: | |
| Online Access: | https://www.aimspress.com/article/doi/10.3934/microbiol.2022003?viewType=HTML |
| _version_ | 1818050783873073152 |
|---|---|
| author | Alaa Emara Rabee Khalid Z. Kewan Hassan M. El Shaer Mebarek Lamara Ebrahim A. Sabra |
| author_facet | Alaa Emara Rabee Khalid Z. Kewan Hassan M. El Shaer Mebarek Lamara Ebrahim A. Sabra |
| author_sort | Alaa Emara Rabee |
| collection | DOAJ |
| description | Rumen methanogens prevent the accumulation of fermentation gases in the rumen and generate methane that increases global warming and represents a loss in animals' gross energy. Non-traditional feed resources such as the by-products of date palm (Phoenix dactylifera) and olive (Olea europaea) trees have received attention to be used in animal feeding. This study evaluated the impact of non-traditional feed resources including olive cake (OC), discarded dates (DD), and date palm frond (DPF) in sheep diet on rumen fermentation, diversity and relative abundance of rumen methanogens. Nine adult rams were assigned to three equal groups and fed three diets: traditional concentrates mixture (S1); non-traditional concentrate mixture (S2) based on DD and OC; and (S3) composed of the same S2 concentrate supplemented with DPF as a roughage part. The results showed that rumen pH was higher with S3 diet than the other two diets. However, the S1 diet showed the highest values of total volatile fatty acids (TVFA) and rumen ammonia. In addition, the proportions of acetic and butyric acids were increased, whereas propionic acid declined in S2 and S3 compared to the S1 diet. Rumen methanogens were dominated by Methanobrevibacter that showed a numeric decline by including DD, OC, and DPF in the animal diets. Principal component analysis (PCA) based on rumen fermentation parameters and relative abundances of methanogens genera showed three distinct clusters. Also, positive and negative correlations were revealed between methanogens genera and rumen metabolites. This study expands the knowledge regarding the effect of agricultural byproducts on rumen fermentation and the methanogenic community. |
| first_indexed | 2024-12-10T10:58:58Z |
| format | Article |
| id | doaj.art-d002a61edeed4422aded8b973341f163 |
| institution | Directory Open Access Journal |
| issn | 2471-1888 |
| language | English |
| last_indexed | 2024-12-10T10:58:58Z |
| publishDate | 2022-01-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Microbiology |
| spelling | doaj.art-d002a61edeed4422aded8b973341f1632022-12-22T01:51:45ZengAIMS PressAIMS Microbiology2471-18882022-01-0181264110.3934/microbiol.2022003Effect of olive and date palm by-products on rumen methanogenic community in Barki sheepAlaa Emara Rabee 0Khalid Z. Kewan 1Hassan M. El Shaer 2Mebarek Lamara3Ebrahim A. Sabra4 1. Animal and Poultry Nutrition Department, Desert Research Center, Cairo, Egypt 1. Animal and Poultry Nutrition Department, Desert Research Center, Cairo, Egypt1. Animal and Poultry Nutrition Department, Desert Research Center, Cairo, Egypt3. Forest Research Institute, University of Quebec in Abitibi-Temiscamingue, Rouyn-Noranda, Canada2. Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadate City, Menoufia, EgyptRumen methanogens prevent the accumulation of fermentation gases in the rumen and generate methane that increases global warming and represents a loss in animals' gross energy. Non-traditional feed resources such as the by-products of date palm (Phoenix dactylifera) and olive (Olea europaea) trees have received attention to be used in animal feeding. This study evaluated the impact of non-traditional feed resources including olive cake (OC), discarded dates (DD), and date palm frond (DPF) in sheep diet on rumen fermentation, diversity and relative abundance of rumen methanogens. Nine adult rams were assigned to three equal groups and fed three diets: traditional concentrates mixture (S1); non-traditional concentrate mixture (S2) based on DD and OC; and (S3) composed of the same S2 concentrate supplemented with DPF as a roughage part. The results showed that rumen pH was higher with S3 diet than the other two diets. However, the S1 diet showed the highest values of total volatile fatty acids (TVFA) and rumen ammonia. In addition, the proportions of acetic and butyric acids were increased, whereas propionic acid declined in S2 and S3 compared to the S1 diet. Rumen methanogens were dominated by Methanobrevibacter that showed a numeric decline by including DD, OC, and DPF in the animal diets. Principal component analysis (PCA) based on rumen fermentation parameters and relative abundances of methanogens genera showed three distinct clusters. Also, positive and negative correlations were revealed between methanogens genera and rumen metabolites. This study expands the knowledge regarding the effect of agricultural byproducts on rumen fermentation and the methanogenic community.https://www.aimspress.com/article/doi/10.3934/microbiol.2022003?viewType=HTMLsheep rumenmethanogenic archaeaillumina mi seqolive cakediscarded datesdate palm fronds |
| spellingShingle | Alaa Emara Rabee Khalid Z. Kewan Hassan M. El Shaer Mebarek Lamara Ebrahim A. Sabra Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep AIMS Microbiology sheep rumen methanogenic archaea illumina mi seq olive cake discarded dates date palm fronds |
| title | Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep |
| title_full | Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep |
| title_fullStr | Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep |
| title_full_unstemmed | Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep |
| title_short | Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep |
| title_sort | effect of olive and date palm by products on rumen methanogenic community in barki sheep |
| topic | sheep rumen methanogenic archaea illumina mi seq olive cake discarded dates date palm fronds |
| url | https://www.aimspress.com/article/doi/10.3934/microbiol.2022003?viewType=HTML |
| work_keys_str_mv | AT alaaemararabee effectofoliveanddatepalmbyproductsonrumenmethanogeniccommunityinbarkisheep AT khalidzkewan effectofoliveanddatepalmbyproductsonrumenmethanogeniccommunityinbarkisheep AT hassanmelshaer effectofoliveanddatepalmbyproductsonrumenmethanogeniccommunityinbarkisheep AT mebareklamara effectofoliveanddatepalmbyproductsonrumenmethanogeniccommunityinbarkisheep AT ebrahimasabra effectofoliveanddatepalmbyproductsonrumenmethanogeniccommunityinbarkisheep |