Improved rooting capacity and hardening efficiency of carob (Ceratonia siliqua L.) cuttings using arbuscular mycorrhizal fungi
The present investigation was undertaken to improve the performance of carob cuttings in terms of adventitious roots formation and hardening using arbuscular mycorrhizal fungi (AMF). Softwood cuttings were treated with 5000 mg L-1 of indole-3-butyric acid (IBA) and kept noninoculated (Non-A...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600100E.pdf |
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| author | Essahibi Abdellatif Benhiba Laila Oussouf Fouad Mohamed Babram Mohamed Ait Ghoulam Cherki Qaddoury Ahmed |
| author_facet | Essahibi Abdellatif Benhiba Laila Oussouf Fouad Mohamed Babram Mohamed Ait Ghoulam Cherki Qaddoury Ahmed |
| author_sort | Essahibi Abdellatif |
| collection | DOAJ |
| description | The present investigation was undertaken to improve the performance of carob
cuttings in terms of adventitious roots formation and hardening using
arbuscular mycorrhizal fungi (AMF). Softwood cuttings were treated with 5000
mg L-1 of indole-3-butyric acid (IBA) and kept noninoculated (Non-AM) or
inoculated with Funneliformis mosseae (Fmo) alone or combined with
Rhizophagus fasciculatus (Fmo+Rfa) or R. intraradices (Fmo+Rin) or both
(Fmo+Rfa+Rin) and then maintained under mist conditions. After two months,
rooted cuttings were transplanted on sterilized substrate and transferred to
a hardening greenhouse for five months. Obtained results showed that
inoculation of the rooting substrate with AMF substantially improved the
percentage of rooted cuttings and the number of roots per cutting. The
highest rooting (63.33%) and number of roots per cutting (11.67) were
recorded in the presence of the complex of the three AMF strains
(Fmo+Rfa+Rin). Moreover, all mycorrhizal-rooted cuttings survived
transplantation and hardening shocks and showed the highest growth and
physiological performances. Indeed, in the Fmo-Rfa-Rin-plantlets the gains in
plant height and shoot and root dry weights were 95.6%, 55.1% and 76.9%
respectively. Furthermore, stomatal conductance, total chlorophyll content,
photochemical efficiency of PSII (Fv/Fm) and nutrient concentrations were
higher in mycorrhizal plantlets than in non-AM ones. Thus, AMF substantially
improved carob cuttings’ performance in terms of rooting capacity and
hardening efficiency, thereby increasing the potential of carob propagation
by cuttings. |
| first_indexed | 2024-12-14T21:51:46Z |
| format | Article |
| id | doaj.art-d00629de10194a39b23a6fcc6e51c141 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-14T21:51:46Z |
| publishDate | 2017-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-d00629de10194a39b23a6fcc6e51c1412022-12-21T22:46:14ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392017-01-0169229129810.2298/ABS160307100E0354-46641600100EImproved rooting capacity and hardening efficiency of carob (Ceratonia siliqua L.) cuttings using arbuscular mycorrhizal fungiEssahibi Abdellatif0Benhiba Laila1Oussouf Fouad Mohamed2Babram Mohamed Ait3Ghoulam Cherki4Qaddoury Ahmed5Cadi Ayyad University, Faculty of Sciences and Techniques, Department of Biology, Unit of Plant Biotechnology and Symbiosis Agrophysiology, Marrakech, MoroccoCadi Ayyad University, Faculty of Sciences and Techniques, Department of Biology, Unit of Plant Biotechnology and Symbiosis Agrophysiology, Marrakech, MoroccoCadi Ayyad University, Faculty of Sciences and Techniques, Department of Biology, Unit of Plant Biotechnology and Symbiosis Agrophysiology, Marrakech, MoroccoCadi Ayyad University, Faculty of Sciences and Techniques, Department of Mathematics, Marrakesh, MoroccoCadi Ayyad University, Faculty of Sciences and Techniques, Department of Biology, Unit of Plant Biotechnology and Symbiosis Agrophysiology, Marrakech, MoroccoCadi Ayyad University, Faculty of Sciences and Techniques, Department of Biology, Unit of Plant Biotechnology and Symbiosis Agrophysiology, Marrakech, MoroccoThe present investigation was undertaken to improve the performance of carob cuttings in terms of adventitious roots formation and hardening using arbuscular mycorrhizal fungi (AMF). Softwood cuttings were treated with 5000 mg L-1 of indole-3-butyric acid (IBA) and kept noninoculated (Non-AM) or inoculated with Funneliformis mosseae (Fmo) alone or combined with Rhizophagus fasciculatus (Fmo+Rfa) or R. intraradices (Fmo+Rin) or both (Fmo+Rfa+Rin) and then maintained under mist conditions. After two months, rooted cuttings were transplanted on sterilized substrate and transferred to a hardening greenhouse for five months. Obtained results showed that inoculation of the rooting substrate with AMF substantially improved the percentage of rooted cuttings and the number of roots per cutting. The highest rooting (63.33%) and number of roots per cutting (11.67) were recorded in the presence of the complex of the three AMF strains (Fmo+Rfa+Rin). Moreover, all mycorrhizal-rooted cuttings survived transplantation and hardening shocks and showed the highest growth and physiological performances. Indeed, in the Fmo-Rfa-Rin-plantlets the gains in plant height and shoot and root dry weights were 95.6%, 55.1% and 76.9% respectively. Furthermore, stomatal conductance, total chlorophyll content, photochemical efficiency of PSII (Fv/Fm) and nutrient concentrations were higher in mycorrhizal plantlets than in non-AM ones. Thus, AMF substantially improved carob cuttings’ performance in terms of rooting capacity and hardening efficiency, thereby increasing the potential of carob propagation by cuttings.http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600100E.pdfCeratonia siliqua Larbuscular mycorrhizal fungicuttingsrootinghardening. |
| spellingShingle | Essahibi Abdellatif Benhiba Laila Oussouf Fouad Mohamed Babram Mohamed Ait Ghoulam Cherki Qaddoury Ahmed Improved rooting capacity and hardening efficiency of carob (Ceratonia siliqua L.) cuttings using arbuscular mycorrhizal fungi Archives of Biological Sciences Ceratonia siliqua L arbuscular mycorrhizal fungi cuttings rooting hardening. |
| title | Improved rooting capacity and hardening efficiency of carob (Ceratonia siliqua L.) cuttings using arbuscular mycorrhizal fungi |
| title_full | Improved rooting capacity and hardening efficiency of carob (Ceratonia siliqua L.) cuttings using arbuscular mycorrhizal fungi |
| title_fullStr | Improved rooting capacity and hardening efficiency of carob (Ceratonia siliqua L.) cuttings using arbuscular mycorrhizal fungi |
| title_full_unstemmed | Improved rooting capacity and hardening efficiency of carob (Ceratonia siliqua L.) cuttings using arbuscular mycorrhizal fungi |
| title_short | Improved rooting capacity and hardening efficiency of carob (Ceratonia siliqua L.) cuttings using arbuscular mycorrhizal fungi |
| title_sort | improved rooting capacity and hardening efficiency of carob ceratonia siliqua l cuttings using arbuscular mycorrhizal fungi |
| topic | Ceratonia siliqua L arbuscular mycorrhizal fungi cuttings rooting hardening. |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600100E.pdf |
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