Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat.
This research aimed to determine the potential of endophytic Trichoderma sp originated from oil palm plant tissue as a biological control agent and its molecular identification. The potency assay was carried out using a completely randomized design consisting of three isolates of Trichoderma sp. fr...
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| Format: | Article |
| Language: | English |
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Universitas Lancang Kuning
2022-07-01
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| Series: | Jurnal Ilmiah Pertanian |
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| Online Access: | http://journal.unilak.ac.id/index.php/jip/article/view/9402 |
| _version_ | 1818492414598316032 |
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| author | Fifi Puspita Titania T. Nugroho Rachmad Saputra |
| author_facet | Fifi Puspita Titania T. Nugroho Rachmad Saputra |
| author_sort | Fifi Puspita |
| collection | DOAJ |
| description |
This research aimed to determine the potential of endophytic Trichoderma sp originated from oil palm plant tissue as a biological control agent and its molecular identification. The potency assay was carried out using a completely randomized design consisting of three isolates of Trichoderma sp. from different plant tissues (TR01 from root tissue, TS01 from stem tissue, and TM01 from midrib tissue) with inhibitory ability parameters. The inhibition of the growth of G. boninense was performed at the concentration of 15% and 20%, and the molecular identification of Trichoderma sp. endophyte isolates was analyzed using BLAST software. The results showed that Trichoderma sp. TM01 isolate had larger inhibitory than the other isolates on days 3 and 4 by 60% and 68%. TM01 isolate also showed inhibition to the growth of the G. boninense at 15% and 20%, with the percentage of inhibition reaching 71.79% and 82.05%. Based on phylogenetic analysis, the three endophytic isolates of Trichoderma sp. were closely related to Trichoderma virens.
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| first_indexed | 2024-12-10T17:42:59Z |
| format | Article |
| id | doaj.art-d00d5882da7040ff8fa87d1c7de518c9 |
| institution | Directory Open Access Journal |
| issn | 1829-8346 2502-5988 |
| language | English |
| last_indexed | 2024-12-10T17:42:59Z |
| publishDate | 2022-07-01 |
| publisher | Universitas Lancang Kuning |
| record_format | Article |
| series | Jurnal Ilmiah Pertanian |
| spelling | doaj.art-d00d5882da7040ff8fa87d1c7de518c92022-12-22T01:39:20ZengUniversitas Lancang KuningJurnal Ilmiah Pertanian1829-83462502-59882022-07-0119210.31849/jip.v19i2.9402Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat.Fifi Puspita0Titania T. Nugroho1Rachmad SaputraDepartment of Agrotechnology, Faculty of Agriculture, Universitas Riau, Pekanbaru, Indonesia Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau, Pekanbaru, Indonesia This research aimed to determine the potential of endophytic Trichoderma sp originated from oil palm plant tissue as a biological control agent and its molecular identification. The potency assay was carried out using a completely randomized design consisting of three isolates of Trichoderma sp. from different plant tissues (TR01 from root tissue, TS01 from stem tissue, and TM01 from midrib tissue) with inhibitory ability parameters. The inhibition of the growth of G. boninense was performed at the concentration of 15% and 20%, and the molecular identification of Trichoderma sp. endophyte isolates was analyzed using BLAST software. The results showed that Trichoderma sp. TM01 isolate had larger inhibitory than the other isolates on days 3 and 4 by 60% and 68%. TM01 isolate also showed inhibition to the growth of the G. boninense at 15% and 20%, with the percentage of inhibition reaching 71.79% and 82.05%. Based on phylogenetic analysis, the three endophytic isolates of Trichoderma sp. were closely related to Trichoderma virens. http://journal.unilak.ac.id/index.php/jip/article/view/9402endophytic fungiGanoderma boninensemolecular characteristicsoil palm tissueTrichoderma virens |
| spellingShingle | Fifi Puspita Titania T. Nugroho Rachmad Saputra Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. Jurnal Ilmiah Pertanian endophytic fungi Ganoderma boninense molecular characteristics oil palm tissue Trichoderma virens |
| title | Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. |
| title_full | Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. |
| title_fullStr | Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. |
| title_full_unstemmed | Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. |
| title_short | Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. |
| title_sort | biological control agents potential and molecular identification of endophytic trichoderma isolates originated from oil palm tissue against ganoderma boninense pat |
| topic | endophytic fungi Ganoderma boninense molecular characteristics oil palm tissue Trichoderma virens |
| url | http://journal.unilak.ac.id/index.php/jip/article/view/9402 |
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