Detection of Mouse Cytomegalovirus in Adenocarcinoma Bearing Razi/A Mice: Molecular and Pathological Studies
Despite a lot of research, the etiology and progression of breast cancer remain incompletely understood. Recently, human cytomegalovirus (HCMV) was reported as a risk factor for breast cancer. The aim of this study was to know whether breast cancer could be caused by cytomegalovirus or not? In this...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Razi Vaccine and Serum Research Institute
2014-05-01
|
| Series: | Archives of Razi Institute |
| Subjects: | |
| Online Access: | http://www.archrazi.com/browse.php?a_id=473&sid=1&slc_lang=en |
| _version_ | 1811283026997411840 |
|---|---|
| author | Abedini, F., Moharrami, M., Shams, P., Eslampanah, M., Adeldost, H., Ebrahimi, M., Vaziri, A., Talebloo, F. |
| author_facet | Abedini, F., Moharrami, M., Shams, P., Eslampanah, M., Adeldost, H., Ebrahimi, M., Vaziri, A., Talebloo, F. |
| author_sort | Abedini, F., |
| collection | DOAJ |
| description | Despite a lot of research, the etiology and progression of breast cancer remain incompletely understood. Recently, human cytomegalovirus (HCMV) was reported as a risk factor for breast cancer. The aim of this study was to know whether breast cancer could be caused by cytomegalovirus or not? In this experiment seventeen samples of RAZI/A mice with spontaneous breast cancer were being gathered from laboratory animals department. Histopathology and polymerase chain reaction (PCR) tests were done on breast tissue samples. Formalin-fixed tissue specimens were obtained from mouse normal breast tissues (n:17) and mouse mammary tumors (n:17). Detection of mouse cytomegalovirus was done by the pUC57-MCK-2 plasmid. Our histopathology data showed Adenocarcinoma type B in mouse with mammary tumors. There was a significant difference between mice with spontaneous breast cancer and control by Pearson Chi-Square (Value: 17.000b and P=0.000). More research will be needed to determine the effect of cytomegalovirus on breast cancer. |
| first_indexed | 2024-04-13T02:04:20Z |
| format | Article |
| id | doaj.art-d00e798ea9724702b76bb47107cb2c44 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-04-13T02:04:20Z |
| publishDate | 2014-05-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-d00e798ea9724702b76bb47107cb2c442022-12-22T03:07:31ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722014-05-016918388Detection of Mouse Cytomegalovirus in Adenocarcinoma Bearing Razi/A Mice: Molecular and Pathological StudiesAbedini, F., 0Moharrami, M., 1Shams, P., 2Eslampanah, M., 3Adeldost, H., 4Ebrahimi, M., 5Vaziri, A., 6Talebloo, F.7Department of Animal Breeding, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Animal Breeding, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Microbiology, Islamic Azad University, Tonekabon Branch, Mazanderan, IranDepartment of Pathology, Razi Vaccine and Serum Research Institute, Karaj, IranFaculty of Animal sciences, Tehran University, Tehran, IranDepartment of Quality assurance, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Virology, Faculty of virology, Kurdistan University, Sanandaj, IranDepartment of Pathology, Razi Vaccine and Serum Research Institute, Karaj, IranDespite a lot of research, the etiology and progression of breast cancer remain incompletely understood. Recently, human cytomegalovirus (HCMV) was reported as a risk factor for breast cancer. The aim of this study was to know whether breast cancer could be caused by cytomegalovirus or not? In this experiment seventeen samples of RAZI/A mice with spontaneous breast cancer were being gathered from laboratory animals department. Histopathology and polymerase chain reaction (PCR) tests were done on breast tissue samples. Formalin-fixed tissue specimens were obtained from mouse normal breast tissues (n:17) and mouse mammary tumors (n:17). Detection of mouse cytomegalovirus was done by the pUC57-MCK-2 plasmid. Our histopathology data showed Adenocarcinoma type B in mouse with mammary tumors. There was a significant difference between mice with spontaneous breast cancer and control by Pearson Chi-Square (Value: 17.000b and P=0.000). More research will be needed to determine the effect of cytomegalovirus on breast cancer.http://www.archrazi.com/browse.php?a_id=473&sid=1&slc_lang=enMouse CytomegalovirusBreast CancerHuman cytomegalovirusMCK-2 Gene |
| spellingShingle | Abedini, F., Moharrami, M., Shams, P., Eslampanah, M., Adeldost, H., Ebrahimi, M., Vaziri, A., Talebloo, F. Detection of Mouse Cytomegalovirus in Adenocarcinoma Bearing Razi/A Mice: Molecular and Pathological Studies Archives of Razi Institute Mouse Cytomegalovirus Breast Cancer Human cytomegalovirus MCK-2 Gene |
| title | Detection of Mouse Cytomegalovirus in Adenocarcinoma Bearing Razi/A Mice: Molecular and Pathological Studies |
| title_full | Detection of Mouse Cytomegalovirus in Adenocarcinoma Bearing Razi/A Mice: Molecular and Pathological Studies |
| title_fullStr | Detection of Mouse Cytomegalovirus in Adenocarcinoma Bearing Razi/A Mice: Molecular and Pathological Studies |
| title_full_unstemmed | Detection of Mouse Cytomegalovirus in Adenocarcinoma Bearing Razi/A Mice: Molecular and Pathological Studies |
| title_short | Detection of Mouse Cytomegalovirus in Adenocarcinoma Bearing Razi/A Mice: Molecular and Pathological Studies |
| title_sort | detection of mouse cytomegalovirus in adenocarcinoma bearing razi a mice molecular and pathological studies |
| topic | Mouse Cytomegalovirus Breast Cancer Human cytomegalovirus MCK-2 Gene |
| url | http://www.archrazi.com/browse.php?a_id=473&sid=1&slc_lang=en |
| work_keys_str_mv | AT abedinif detectionofmousecytomegalovirusinadenocarcinomabearingraziamicemolecularandpathologicalstudies AT moharramim detectionofmousecytomegalovirusinadenocarcinomabearingraziamicemolecularandpathologicalstudies AT shamsp detectionofmousecytomegalovirusinadenocarcinomabearingraziamicemolecularandpathologicalstudies AT eslampanahm detectionofmousecytomegalovirusinadenocarcinomabearingraziamicemolecularandpathologicalstudies AT adeldosth detectionofmousecytomegalovirusinadenocarcinomabearingraziamicemolecularandpathologicalstudies AT ebrahimim detectionofmousecytomegalovirusinadenocarcinomabearingraziamicemolecularandpathologicalstudies AT vaziria detectionofmousecytomegalovirusinadenocarcinomabearingraziamicemolecularandpathologicalstudies AT talebloof detectionofmousecytomegalovirusinadenocarcinomabearingraziamicemolecularandpathologicalstudies |