Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for <i>Clostridium difficile</i> Toxin B Detection
<i>Clostridium difficile</i> colitis is caused by a cytotoxin produced by the anaerobic bacteria <i>C. difficile</i> in the epithelial cells of the large intestine, particularly <i>C. difficile</i> toxin B (TcdB). However, the sensitivity of currently utilized <...
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MDPI AG
2020-11-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/11/722 |
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| author | Haonan Qi Qiuli Sun Yi Ma Peidian Wu Jufang Wang |
| author_facet | Haonan Qi Qiuli Sun Yi Ma Peidian Wu Jufang Wang |
| author_sort | Haonan Qi |
| collection | DOAJ |
| description | <i>Clostridium difficile</i> colitis is caused by a cytotoxin produced by the anaerobic bacteria <i>C. difficile</i> in the epithelial cells of the large intestine, particularly <i>C. difficile</i> toxin B (TcdB). However, the sensitivity of currently utilized <i>C. difficile</i> endotoxin determination methods has been called into question, and, therefore, more accurate and convenient detection methods are needed. Our study is the first to systematically compare fluorescent submicrosphere-based and quantum-dot nanobead-based lateral fluidity measurement methods (FMs-LFA and QDNBs-LFA) with toxin B quantification in fecal samples via sandwich analysis. The limits of detection (LOD) of FMs-LFA and QDNBs-LFA in the fecal samples were 0.483 and 0.297 ng/mL, respectively. TcdB analyses of the fecal samples indicated that the results of QDNBs-LFA and FMs-LFA were consistent with those of a commercial enzyme-linked immunosorbent assay (ELISA) test kit. The sensitivity of QDNBs-LFA was highly correlated with clinical diagnoses. Therefore, quantum dot nanobeads (QDNBs) are deemed highly suitable for lateral fluidity analyses, which would facilitate the implementation of portable and rapid on-the-spot applications, such as food hygiene and safety tests and onsite medical testing. |
| first_indexed | 2024-03-10T14:45:13Z |
| format | Article |
| id | doaj.art-d00fa327c83544cc8451e89df4658ebf |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T14:45:13Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d00fa327c83544cc8451e89df4658ebf2023-11-20T21:28:44ZengMDPI AGToxins2072-66512020-11-01121172210.3390/toxins12110722Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for <i>Clostridium difficile</i> Toxin B DetectionHaonan Qi0Qiuli Sun1Yi Ma2Peidian Wu3Jufang Wang4School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, ChinaNational & Local United Engineering Lab of Rapid Diagnostic Test, Guangzhou Wondfo Biotech Co., Ltd., Guangzhou 510663, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China<i>Clostridium difficile</i> colitis is caused by a cytotoxin produced by the anaerobic bacteria <i>C. difficile</i> in the epithelial cells of the large intestine, particularly <i>C. difficile</i> toxin B (TcdB). However, the sensitivity of currently utilized <i>C. difficile</i> endotoxin determination methods has been called into question, and, therefore, more accurate and convenient detection methods are needed. Our study is the first to systematically compare fluorescent submicrosphere-based and quantum-dot nanobead-based lateral fluidity measurement methods (FMs-LFA and QDNBs-LFA) with toxin B quantification in fecal samples via sandwich analysis. The limits of detection (LOD) of FMs-LFA and QDNBs-LFA in the fecal samples were 0.483 and 0.297 ng/mL, respectively. TcdB analyses of the fecal samples indicated that the results of QDNBs-LFA and FMs-LFA were consistent with those of a commercial enzyme-linked immunosorbent assay (ELISA) test kit. The sensitivity of QDNBs-LFA was highly correlated with clinical diagnoses. Therefore, quantum dot nanobeads (QDNBs) are deemed highly suitable for lateral fluidity analyses, which would facilitate the implementation of portable and rapid on-the-spot applications, such as food hygiene and safety tests and onsite medical testing.https://www.mdpi.com/2072-6651/12/11/722lateral flow assayfluorescent submicrospheresquantum dot nanobeadtoxin B |
| spellingShingle | Haonan Qi Qiuli Sun Yi Ma Peidian Wu Jufang Wang Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for <i>Clostridium difficile</i> Toxin B Detection Toxins lateral flow assay fluorescent submicrospheres quantum dot nanobead toxin B |
| title | Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for <i>Clostridium difficile</i> Toxin B Detection |
| title_full | Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for <i>Clostridium difficile</i> Toxin B Detection |
| title_fullStr | Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for <i>Clostridium difficile</i> Toxin B Detection |
| title_full_unstemmed | Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for <i>Clostridium difficile</i> Toxin B Detection |
| title_short | Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for <i>Clostridium difficile</i> Toxin B Detection |
| title_sort | advantages of lateral flow assays based on fluorescent submicrospheres and quantum dots for i clostridium difficile i toxin b detection |
| topic | lateral flow assay fluorescent submicrospheres quantum dot nanobead toxin B |
| url | https://www.mdpi.com/2072-6651/12/11/722 |
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